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Biomedical subjects

J C Lin

Publications and source records attributed to J C Lin.

At least 289 records · Page 16Linked to original sources
PubMed1981

Immunological detection of metastases from prostatic adenocarcinoma.

In six patients with known prostatic adenocarcinoma, extraprostatic (metastatic) tumor was suspected on radiographic or radionucleotide studies. When cytological examination of tissue obtained by needle aspiration or biopsy was nondiagnostic, radial gel immunodiffusion was used to identify the presence of prostatic acid phosphatase in the tissue. Four specimens demonstrated prostate-specific acid phosphatase activity, permitting the diagnosis of metastatic prostatic adenocarcinoma. The technique is simple and highly specific.

Acid Phosphatase↗
PubMed1981

Axillary F-loop latency of ulnar nerve in normal young adults.

F waves were studied with stimulation of the ulnar nerve in normal subjects (25 men and 15 women, with an average age of 26.5 years) using an EMG machine that permits the simultaneous display of 8 sweeps. The axillary F-loop latency (AFLL), defined at the conduction time between the axilla (25cm from the sternal notch) and the spinal cord, was 9.17 +/- 0.81msec when 8 F responses were measured but was 8.57 +/- 1.00msec when 40 F responses were used. Data from men and women showed no significant statistical difference. The mean plus 2 standard deviations were 10.8 and 10.6msec for 8 and 40 responses, respectively. On the basis of these findings, an AFLL longer than 11msec should be considered abnormal in the young adult. Four measures repeated at weekly intervals showed no significant difference from week to week, indicating that the technique can be consistently and reliably applied at different times. The average duration of the F complex was 15.78 +/- 2.55msec and was about 80% of the average duration of the M wave.

Adolescent↗
PubMed1980

Studies on microwave and blood-brain barrier interaction.

This investigation was aimed at correlating changes of blood-brain-barrier permeability with the quantity and distribution of absorbed microwave energy inside the brain of adult Wistar rats anesthetized by sodium pentobarbital. Through use of thermographic methods and a direct-contact applicator at the animal's head, the pattern of absorbed microwave energy was determined. Indwelling catheters were placed in the femoral vein and in the left external carotid artery. Evans blue and sodium fluorescein in isotonic saline were used as visual indicators of barrier permeation. Exposure to pulsed 2,450-MHz radiation for 20 min at average power densities of 0.5, 1, 5, 20, 145 or 1,000 mW/cm2, which resulted in average specific absorption rates (SARs) of 0.04, 0.08, 0.4, 1.6, 11.5 or 80.0 mW/g in the brain, did not produce staining, except in the pineal body, the pituitary gland, and the choroid plexus - regions that normally are highly permeable. Except for these regions, staining was also absent in the brains of sham-exposed animals. The rectal temperature, as monitored by a copper-constantan thermocouple, showed a maximum increase of less than 0.75 degrees C from a mean pre-exposure temperature of 36.6 degrees C. The highest brain temperature recorded in a similar group of animals using a thickfilm carbon thermistor was less than 41.0 degrees C.

Absorption↗
PubMed1980

Purification and partial characterization of a preneoplastic antigen in liver carcinogenesis.

A preneoplastic antigen has been isolated from the microsomal fraction of the hyperplastic nodules of rat liver. During the purification, the presence of preneoplastic antigen was monitored by microcomplement fixation test and double immunodiffusion plate following reaction with antiserum specific for this antigen. The antigen was purified by seven steps with a purification factor of 167 and 11% yield. It migrated as a single protein band, both in nondenaturing and denaturing polyacrylamide gel electrophoresis; showed a single sharp peak in an isoelectric focusing column at pH 4.75; and had a single serine amino-terminal amino acid. In the absence of detergent during purification, this protein tends to form molecular aggregates. It appears to be a glycoprotein based on the positive periodic acid-Schiff stain and absorption by concanavalin A:Sepharose affinity column chromatography. Amino acid analysis indicated that the protein is acidic, having acidic amino acids (aspartate and glutamate) and basic amino acids (lysine, arginine, and histidine) in a ratio of 6.8. An estimate of the molecular weight by gel filtration gave a value of 145,000 and a calculated Stokes radius of 41.5 A. Electrophoresis under denaturing conditions on sodium dodecyl sulfate-polyacrylamide gel indicated that this protein is a dimer consisting of two subunits of equal molecular weight (M.W. 74,000). Circular dichroism spectra indicated alpha-helix contents at 298 and 222 nm of 25.5 and 28.3%, respectively. A similar protein is present in the endoplasmic reticulum of normal rat liver, but in a much lower concentration.

Amino Acids↗
PubMed1979

Microwave-evoked brainstem potentials in cats.

Rectangular pulse-modulated microwave radiation has been shown to produce auditory responses in mammals. It is therefore reasonable to explore the possibility of using microwave pulses to achieve an estimate of sensori-neural involvement in the objective evaluation of human hearing and to assess the presence of tumors or brainstem lesions in patients with neurological disorders. In this paper we shall show that microwave-evoked auditory response of cats closely resembles that evoked by acoustic pulse. We shall also give preliminary results obtained from electrodes fastened to the vertex of the skull after successive coagulative production of lesions in the inferior colliculus, lateral lemniscus, and superior olivary nucleus.

Acoustic Stimulation↗
PubMed1978

In vivo growth of human bladder cancer cell lines.

Two human bladder cancer cell lines grew predictably in rats immunosuppressed with antilymphocyte serum. Intraperitoneal inoculation of tumor cell suspensions resulted in diffuse intraabdominal carcinomatosis with consequent host death after 10 to 20 days. Subcutaneous inoculation of tumor cell suspensions resulted in local tumors which grew exponentially for 20 to 30 days before eventual regression after 40 to 50 days; lung metastases developed in at least 13 per cent of the animals with subcutaneous tumors. The histologic appearance of the xenografted tumors closely resembled that of the original tumors. Subsequent in vitro culture of the xenografted tumors provided cell lines that were morphologically identical to the primary lines and that retained a human karyotype. It is proposed to employ this model of human bladder cancer to evaluate chemotherapeutic agents for possible use in the clinical disease.

Animals↗