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Biomedical subjects

J C Hwang

Publications and source records attributed to J C Hwang.

At least 55 records · Page 3Linked to original sources
PubMed1989

Effect of electrical stimulation of the superior laryngeal nerve on respiratory-modulated facial nerve activity in cats.

The purpose of the present study was to evaluate the response of respiratory-modulated facial nerve to electrical activation on the superior laryngeal nerve (SLN). The experiments were performed on fifteen cats which were anesthetized with pentobarbital, vagotomized, paralyzed and artificially ventilated. The animals were maintained at different levels of carbon dioxide in hyperoxia. Parameters of electrical stimulation were 20 Hz of frequency, 0.5 mSec of duration and a variety of intensity (30, 50, 100 and 150 microA). Facial activity increased significantly (P less than 0.05) in response to SLN activation. This augmentation of facial discharge was proportional to the intensity of electrical activation on the SLN. Phrenic response to SLN stimulation was either decreased or not changed. Total duration of respiratory cycle after activation of the SLN was increased. The results showed that facial activity may play a role in the regulation of nasal resistance.

Animals↗
PubMed1988

Regeneration studies on a crayfish neuromuscular system. I. Connectivity changes after intersegmental nerve transplants.

The superficial flexor muscles of the crayfish are a neuromuscular system of a few muscle cells innervated by six neurons in a precise position-dependent pattern. The neurons are capable of regenerating their normal connectivity patterns within a short span of time when conditions are favorable. The superficial flexor muscles of the second and third segments, despite their similarities in neuronal and muscle cell size and number, have distinctive connectivity patterns; some homologous neurons form similar patterns but other homologous neurons form patterns that are reversed between segments. We transplanted each segment's nerve into each other's muscle in order to observe regeneration of the nerves into a target area that differed in connectivity patterns from their original muscle. During the first weeks of regeneration all neurons formed a connectivity pattern with more connections medially and declining connections laterally, a pattern determined by the medial location of the nerve transplant. This pattern is maintained for most of the neurons, but for some there is an eventual reduction in medial connections as maximum synapse formation shifts to the lateral muscle fibers. Three of the eight neurons studied were able to regenerate connectivity patterns that corresponded to their segment of origin and not to their host muscle. This suggests that intersegmental muscle differences are not influencing the formation of these connectivity patterns, so the neurons will follow their inherent synaptogenesis program.

Animals↗
PubMed1988

Characterization of respiratory-related activity of the facial nerve.

Activities of the facial, hypoglossal and phrenic nerves were recorded in decerebrate and paralyzed cats. These animals were ventilated with a servo-respirator which produced lung inflations in parallel with phrenic activity. Peak inspiratory phrenic, hypoglossal and facial activities increased in hypercapnia or hypoxia. When pulmonary inflation was prevented, hypoglossal and facial activities increased more than phrenic. Responses to withholding lung inflation differed from those following vagotomy. These differences were observed in expiratory facial and hypoglossal activities and in hypercapnia- and hypoxia-induced changes in facial activity. Administration of pentobarbital or hyperventilation to hypocapnia caused greater suppressions of hypoglossal than facial activity; the latter declined more than phrenic activity. The results support the hypothesis that influences from the brainstem reticular formation and from pulmonary stretch receptors are differentially distributed to motoneurons innervating upper airway muscles compared to those of the bulbospinal-phrenic system. The concept that ventilatory activity is influenced by tonic, as well as phasic discharge of pulmonary receptors is discussed.

Anesthesia↗
PubMed1988

Respiratory-modulated activities of motor units of the facial nerve.

The purpose of this work was to characterize the influence of activity of vagal pulmonary receptors upon the discharge pattern of motor units of the facial nerve. Decerebrate and paralyzed cats were ventilated with a servo-respirator which produced pulmonary inflations in parallel with activity of the phrenic nerve. At normocapnia, facial units discharged phasically during neural inspiration, expiration or across both phases or discharged tonically throughout the respiratory cycle. When pulmonary inflation was withheld, the tonic discharge of some units became phasic; others changed the pattern of phasic discharge. In hypercapnia, the number of tonic fiber activities increased and, again, some phasic discharge patterns were altered. Withholding inflation caused similar alterations as in normocapnia. Activities of facial fibers in vagotomized animals differed in that no tonic activities were recorded, and no change in phasic discharge patterns was induced by hypercapnia. We conclude that afferents from pulmonary stretch receptors influence ventilatory activity throughout the entire respiratory cycle. The concept is discussed that the tonic, as well as phasic discharge of these receptors, is important for the regulation of activity of motoneurons to upper airway muscles.

Animals↗
PubMed1988

Maximum length sequence applied to the measurement of brainstem auditory evoked responses.

An evoked response may be assumed to consist of synchronous electrical activities of groups of neurons in response to a stimulus. Unfortunately, this response is usually weak and often masked by the background EEG activity generated spontaneously by the brain. In order to extract the embedded evoked response we have employed the theory of maximum length sequence, assuming only a temporal linearity of the system from which measurements are taken. The technique has been applied successfully with the aid of a microcomputer. The system also supports the same measurement by a conventional averaging technique. The results of the two are compared, indicating the advantages of the maximum length technique: improved signal-to-noise ratio, repeatability and speed.

Animals↗
PubMed1987

Response characteristics of neurons in the cat vestibular nuclei during slow and constant velocity off-vertical axes rotations in the clockwise and counterclockwise rotations.

The responses to slow constant velocity rotations in the clockwise (CW) and counterclockwise (CCW) directions about an axis tilted 10 degrees from the earth's vertical were studied in static tilt-sensitive neurons in the vestibular nuclei of decerebrate cats. Each unit responded to any 360 degrees unidirectional rotation with a position-dependent discharge maximum. The location of the maximum, obtained by rotation in one direction, differed from that obtained by an oppositely directed rotation (phase difference). In about 80% of the units such phase difference (up to 160 degrees in second-order neurons) in response to oppositely directed rotations was unaffected by different amplitudes of head displacement (5-25 degrees). Units were thus classified into two groups depending on the location of the CW discharge maximum relative to the CCW counterpart. The direction of rotation had no influence on the response gains of these units.

Animals↗
PubMed1987

Alterations of hypoglossal motoneuronal activities during pulmonary inflations.

Preventing pulmonary inflation during inspiration results in greater augmentations in activity of the hypoglossal nerve than in the phrenic nerve. Our purpose was to characterize the hypoglossal motoneuronal activities which underlie these augmentations. Activities of the phrenic and hypoglossal nerves and single hypoglossal fibers were recorded in decerebrate and paralyzed cats. Ventilation was by a servo-respirator which produced changes in lung volume in parallel with phrenic activity. The number of motoneurons that discharged during cycles in which the lungs were inflated increased with elevations of end-tidal fractional concentrations of CO2 (FETCO2) from 0.05 to 0.06 and 0.09. At each FETCO2, the discharge frequency increased when pulmonary inflation was withheld. In addition, withholding inflation resulted in the recruitment of other motoneuronal activities. Most motoneurons discharged during the period of the phrenic burst (inspiratory neurons). Lesser numbers of inspiratory-expiratory, expiratory-inspiratory, and tonic motoneuronal activities were also recorded. Results are considered in the context of the inhibition of respiratory motoneuronal activity by vagal pulmonary afferent fibers. The possible role of such inhibition, and release from this inhibition, in maintenance of patency of the upper airways is discussed.

Action Potentials↗
PubMed1987

Influence of pulmonary inflations on discharge patterns of phrenic motoneurons.

The purpose of this study was to assess the influence of pulmonary inflations on activities of single phrenic motoneurons. Studies were performed in decerebrate and paralyzed cats; activities of phrenic nerve and single phrenic motoneurons were recorded. Animals were ventilated with a servo-respirator which produced alterations in tracheal pressure in parallel with changes in integrated activity of the phrenic nerve. At end-tidal fractional concentrations of CO2 of 0.05, phrenic motoneurons were distributed into "early" and "late" populations, depending on time of onset of activity. During the late stages of neural inspiration, differences in levels of integrated activity of the phrenic nerve became evident between cycles with and without lung inflations. At a time approximating 90% of the inspiratory duration during inflations, integrated phrenic activity was higher for cycles with inflation. Concomitantly, with lung inflations, the discharge frequencies of early phrenic motoneurons were lower, and late motoneurons began to discharge sooner than when inflations were withheld. Similar results were obtained in hypercapnia. We conclude that reflexes activated by pulmonary inflations may produce augmentation, as well as inhibition of phrenic motoneuronal activities. Factors responsible for eliciting these reflex augmentations and inhibitions are discussed.

Animals↗
PubMed1986

Effects of intrapleural pressure on phrenic nerve activity.

In order to evaluate the effect of changing respiratory mechanics on the relation between respiratory mechanical output and phrenic activity, we simultaneously measured ventilatory output from the trace of pneumotachograph and changes in phrenic nerve activity before and after pneumothorax in alpha-chloralose anesthetized dogs. After pneumothorax, the dynamic lung compliance, inspiratory duration and tidal volume were decreased significantly, whereas the integrated peak amplitude and the rate of rise of phrenic neural output were increased substantially. The difference between respiratory mechanical output and phrenic activity was also significant (p less than 0.05). As shown from the results, the increase of phrenic response to pneumothorax may be mediated through intrapulmonary receptors along the vagal afferent rather than through the changes in chemical drive.

Animals↗
PubMed1986

Effects of superfusion of morphine and enkephalins on the activity of single units in the spinal trigeminal nucleus and cuneate nucleus of cat.

The effects of superfusion of morphine, met-enkephalin and D-ala2-met5-enkephalinamide on the spontaneous neural discharge rates of units in the spinal trigeminal nucleus and cuneate nucleus of decerebrate cats were studied. The drugs were superfused onto the dorsum of the exposed surface of the caudal medulla overlying these nuclei. Some of these neurons were identified by their response to innocuous mechanical stimuli delivered to the skin. In the caudal spinal trigeminal nucleus, morphine caused a dose-dependent suppression of the spontaneous discharge rate in the majority of the neurons studied. Endogenous opiate peptide, met-enkephalin or its synthetic analogue, D-ala2-met5-enkephalinamide caused an initial reduction, followed by a rebound of the discharge rate to the control value. These depressant effects of morphine and enkephalins were antagonized by concomitant superfusion of the opiate antagonist naloxone. In the main cuneate nucleus, however, similar doses of morphine, met-enkephalin and D-ala2-met5-enkephalinamide have little if any significant effect on the spontaneous activity of the neurons studied. These results provide electrophysiological evidence for the presence of opiate receptors in the caudal spinal trigeminal nucleus and the relative lack of such receptors in the main cuneate nucleus.

Action Potentials↗
PubMed1985

Effect of tilt on the response of neuronal activity within the cat vestibular nuclei during slow and constant velocity rotation.

The responses of neurons sensitive to static tilt in the vestibular nuclei were examined in decerebrate cats during slow and constant velocity rotations about an axis tilted at various angles from the vertical. During any 360 degrees rotation, each unit showed a modulation of their firing rate, with a position-dependent maximum and minimum. Changes in amplitude of head displacement from 5 degrees to 25 degrees decreased the response gain of the units without affecting the locations of the discharge maxima and minima.

Animals↗
PubMed1984

Afferent pathways for hypoglossal and phrenic responses to changes in upper airway pressure.

Our purpose was to determine the afferent pathways underlying reflexes by which changes in upper airway pressure induced alterations in hypoglossal and phrenic nerve activities. An isolated upper airway was produced in decerebrate, vagotomized, paralyzed and ventilated cats. Efferent activities of the phrenic and hypoglossal nerves were monitored. Hypoglossal activity significantly increased following pressure changes in the upper airway of -4 to -21 cm H2O; phrenic discharge declined in most trials. Similar alterations of neural activities were induced by positive pressures though changes of +14 to +21 cm H2O were required for significant responses. These changes in hypoglossal and phrenic activities were greatly reduced following bilateral sectionings of the superior laryngeal nerves but were augmented after the pharyngeal branches of the glossopharyngeal nerves were sectioned. Additional bilateral destruction of the trigeminal nerves almost entirely eliminated responses to pressure changes. We conclude that upper airway receptors may serve to maintain patency of the upper airways. These receptors may play a crucial role in promoting release from upper airway obstructions, especially in sleep.

Afferent Pathways↗
PubMed1984

Receptors responding to changes in upper airway pressure.

Our purpose was to characterize receptors which respond to changes in upper airway pressures. Such changes cause alterations in hypoglossal and phrenic nerve activities. Decerebrate, vagotomized, paralyzed and ventilated cats were prepared so that pressures could be altered within segments of upper airways. Activities of single fibers in the superior laryngeal and glossopharyngeal nerves were monitored. Most superior laryngeal receptors discharged tonically at zero transmural pressure. Discharges of approximately half decreased (A) and the rest increased (B) with pressure reductions of -7 to -28 cm H2O. Pressure increases of +7 to +28 cm H2O caused increases in Group A activities while Group B responses varied. The remaining receptors were silent, being activated by pressure decreases and/or increases. Activities of other silent receptors, similarly activated, were recorded from glossopharyngeal nerve. Tonically active glossopharyngeal receptors increased discharge after both pressure increases and decreases. Most tonically active and silent receptors, having afferents in either nerve, adapted incompletely to sustained pressures. These may have major functions in hypoglossal responses to changes in upper airway pressures.

Animals↗
PubMed1984

A microcomputer-based binaural measurement system.

A microcomputer is used to control a dual-channel, sine-wave generator as the excitation to both the left and right ears in experimental subjects, and for on-line measurement of the neuronal spike counts. The effects of varying the walking pattern and phase angle between the two channels can be investigated. After normalisation of spike counts, the result can be displayed as a 3-D plot on a CRT or an x-y plotter. The system is useful for general steady state and transient binaural measurement.

Biomedical Engineering↗
PubMed1984

Detection of interaural time differences for clicks and tone pips: effects of interaural signal disparity.

The ability to detect small interaural time differences (delta t) was determined in 4 subjects using clicks or long tone pips of various interaural signal disparities which are expressed as the extent of interaural spectral overlaps. The interaural signal disparity was varied by changing (a) the interaural pulse duration difference (delta d) for clicks, or (b) the interaural carrier frequency difference (delta f) for tone pips. In either case, the sensitivity to delta t was maximal under diotic presentations and declined with delta d or delta f. The overall sensitivity to delta t was remarkably higher for clicks than for long tone pips. The results indicate that both (a) the extent of binaural spectral overlaps and (b) the structure of acoustic stimuli are important in detecting small interaural time differences of binaural sounds.

Acoustic Stimulation↗
PubMed1984

Influence of vasopressin on renal hemodynamics in conscious Brattleboro rats.

The influence of vasopressin on renal hemodynamics was assessed by treating conscious Brattleboro homozygotes (DI rats) both acutely and chronically with physiologic doses of vasopressin. Intravenous infusions of vasopressin for 1 h, resulting in plasma vasopressin concentrations of less than 1.25, 2.3, and 8.0 pg/ml, respectively, failed to change glomerular filtration rate (GFR) or effective renal blood flow (ERBF) significantly, nor were there significant changes during 5 h of infusion. Body weight was not altered during these infusions. When synthetic vasopressin was given by osmotic minipumps for 10 days, with the rats gaining weight and thus changing the volume of their body fluids, GFR and ERBF increased significantly, by approximately 45 and 55%, respectively. Acute administration of volume alone, as well as acute vasopressin plus acute administration of volume, did not alter GFR or ERBF significantly. The data are compatible with the view that vasopressin, in physiologic plasma concentrations, exerts an influence on renal hemodynamics, that may be mediated through the long-term alteration of body fluid volumes. Alternatively or additionally, prolonged exposure of DI rats to vasopressin may increase their renal hemodynamics through tubuloglomerular feedback.

Animals↗