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Biomedical subjects

J C Gardner

Publications and source records attributed to J C Gardner.

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Haemonetics V50 and Plasma Collection System: common concerns and troubleshooting.

The Haemonetics V50 and PCS are automated centrifugal systems designed for the collection of a variety of apheresis products. The microprocessor-controlled systems increase collection efficiency through many safety features and high donor and operator appeal. Troubleshooting the systems has been simplified through a systematic approach and easily understood display messages.

Blood Component Removal

Genes for human vitamin K-dependent plasma proteins C and S are located on chromosomes 2 and 3, respectively.

cDNAs encoding human proteins C and S have been used to screen a panel of mouse-human somatic cell hybrids to determine the chromosomal location of their respective genes. The gene for human protein C is located on chromosome 2, whereas that for protein S is located on chromosome 3. Analysis of human genomic DNA restriction endonuclease fragmentation patterns suggests that the human protein S gene is greater than 40 kb in size and contains a minimum of 11 introns.

Animals

Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms.

A human serum amyloid A (SAA) cDNA was used as a probe in chromosome mapping studies to detect human SAA gene sequences in DNA isolated from human/mouse somatic cell hybrids. Southern analysis of DNA from 20 hybrid cell lines, including some with translocations of human chromosomes, placed the SAA gene(s) in the p11----pter region of chromosome 11. Screening of human DNA from unrelated individuals by Southern analysis using the SAA cDNA probe revealed restriction fragment polymorphisms for HindIII and PstI. An analysis of the segregation of these polymorphisms with other markers on the short arm of chromosome 11 should more precisely map the SAA gene(s).

Amyloid

The Lewin Report.

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Legislation, Nursing