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Biomedical subjects

J C Dombrowski

Publications and source records attributed to J C Dombrowski.

6 recordsLinked to original sources

The comparative analysis of clinically normal and inflamed human gingiva. Study 1: Protein separation by anionic and cationic disc gel electrophoresis.

Homogenates were prepared from sections of both clinically normal and clinically inflamed human gingival tissue. After centrifugation, supernatants were quantitated for protein and subjected to anionic and cationic electrophoresis. Samples of gingival blood and venous blood were similarly subjected to electrophoresis. Significant protein pattern differences are reported for the anionic tissue separations, and for the cationic tissue and plasma separations.

Blood Proteins↗

A rapid chairside test for the severity of periodontal disease using gingival fluid.

A study was done in an attempt to develop a simple test for the severity of periodontal disease. Gingival fluid collected on filter paper was tested for protein content, and the resulting color was compared to standard color filters. Tissue was excised and prepared for histologic examination. The inflammatory cell infiltrate on each slide was graded on a scale of zero to three. Zero was least and three was the highest number of inflammatory cells. The results indicate that the white cell infiltrate graded 0 or 1 on the histologic inflammatory index has a color index of B 1/8 TO B1 whereas the tissue graded 2 or 3 HII has a range of B2 to B6 on the color scale. This test can give the dental practitioner a general idea of the severity of the inflammation.

Color↗