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Biomedical subjects

J C Chang

Publications and source records attributed to J C Chang.

At least 37 records · Page 2Linked to original sources
PubMed1999

The effects of a new ultra-short-acting beta-adrenergic blocker, ONO-1101, on cardiac function during and after cardiopulmonary bypass.

The administration of an ultra-short-acting beta-adrenergic antagonist, esmolol, has been introduced as a novel method for beating-heart surgery. In the present study, a new ultra-short-acting beta-blocker, ONO-1101, was administered during cardiopulmonary bypass (CPB) to investigate its effects on cardiac function and hemodynamics. Nine adult mongrel dogs underwent 60 min of CPB during which they were given either ONO-1101 (ONO group; n = 4) or saline (control group; n = 5). In the ONO group, the hearts became flaccid enough for surgery to be performed without cardiac standstill within 10 min after the commencement of ONO-1101 with significant decreases in the heart rate, the preload recruitable stroke work (PRSW), and the slope of the end-systolic left ventricular pressure-volume relationship (Emax). The mean arterial pressure and systemic vascular resistance also decreased, but were maintained above 50 mmHg during CPB without catecholamine. These indices increased to the control group level 20 min after the discontinuation of ONO-1101. The serum concentration of ONO-1101 decreased from the maximum level of 121 +/- 15 microg/ml soon after infusion to 11 +/- 5 microg/ml within 30 min after discontinuation. These data suggest that ONO-1101 may be useful to enable beating-heart surgery to be performed without aortic cross-clamp as an ultra-short-acting beta-adrenergic blocker.

Adrenergic beta-Antagonists↗
PubMed1999

Evaluation of low-VOC latex paints.

Four commercially available low-volatile organic compound (VOC) latex paints were evaluated as substitutes for conventional latex paints by assessing both their emission characteristics and their performance as coatings. Bulk analysis indicated that the VOC contents of all four paints tested were considerably lower than those of conventional latex paints. Low VOC emissions were confirmed by small chamber emission tests. However, significant emissions of several aldehydes, especially formaldehyde which is a hazardous air pollutant, were detected in emissions from two of the four paints. American Society for Testing and Materials (ASTM) methods were used to evaluate the hiding power, scrub resistance, washability, dry to touch and yellowness index. The results indicated that one of the four low-VOC paints tested showed performance equivalent or superior to that of a conventional latex paint used as control. It was concluded that low-VOC latex paint can be a viable option to replace conventional latex paints for prevention of indoor air pollution. However, paints marketed as "low-VOC" may still have significant emissions of some individual VOCs, and some may not have performance characteristics matching those of conventional latex paints.

Air Pollution, Indoor↗
PubMed1999

Volatile organic compound emissions from latex paint--Part 1. Chamber experiments and source model development.

Latex paints are widely used in residential and commercial indoor environments. The surface areas covered by the paints in these environments are relatively large. Thus, latex paints have the potential for having a major impact on indoor air quality (IAQ). A study was undertaken to develop methods for evaluating the impact of latex paint emission on IAQ. Small chamber experiments using stainless steel and painted and unpainted gypsum board substrates were conducted to determine the emission characteristics of latex paint. The emissions from the stainless steel were relatively short lived (3 to 4 days), whereas the emissions from gypsum board lasted for over 200 days. Because gypsum board is a common substrate for latex paint, all emission models were developed for the gypsum board substrates. The data from the small chamber tests led to the development of two empirical and two mass-transfer-based source emission models. Approximately 100 to 200 days of data were required to estimate the parameters required for the empirical models. Only 8 days of data were required to estimate the parameters for the mass-transfer-based models. The final models use paint formulation and mass transfer correlations to predict the emissions of the major individual volatile organic compounds emitted by latex paint.

Air Pollution, Indoor↗
PubMed1999

Volatile organic compound emissions from latex paint--Part 2. Test house studies and indoor air quality (IAQ) modeling.

Emission models developed using small chamber data were combined with an Indoor Air Quality (IAQ) model to analyze the impact of volatile organic compound (VOC) emissions from latex paint on indoor environments. Test house experiments were conducted to verify the IAQ model's predictions. The agreement between model predictions and experimental measurements met the American Society for Testing and Materials criteria for model verification in the room with the source and met most of the requirements in other rooms. The major cause of disagreement between the model predictions and the experimental data in the test house appears to be an inadequate sink model.

Air Pollution, Indoor↗
PubMed1999

Trends in Missouri lung cancer rates.

This study presents trend analysis of Missouri lung cancer incidence and mortality, and makes comparisons to U.S. rates. Utilizing 1985-1992 data from the Missouri Cancer Registry, we calculated incidence rates by disease diagnosis year. Missouri Center for Health Information Management and Epidemiology data were used to calculate 1980-1996 mortality rates. Missouri's overall yearly lung cancer incidence rate increased from 60.5 to 62.5 per 100,000 during 1985-1992, while lung cancer mortality increased 25% from 1980-1996. The best method to reduce Missouri's rates of lung cancer is tobacco-use prevention and cessation.

Adult↗
PubMed1999

Artificial NS4 mosaic antigen of hepatitis C virus.

An artificial antigen composed of 17 small antigenic regions derived from the NS4-protein of hepatitis C virus (HCV) genotypes 1 through 5 was designed and constructed. Eleven antigenic regions were derived from the 5-1-1 region, and 6 others were derived from the C-terminus of the NS4-protein of different genotypes. The gene encoding for this artificial antigen was assembled from synthetic oligonucleotides by a new approach designated as restriction enzyme-assisted ligation (REAL). The full-length synthetic gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase. By the use of site-specific antibodies raised against synthetic peptides, it was shown that all regions for which sequence-specific antibodies were obtained were accessible to antibody binding. The diagnostic relevance of the NS4 artificial antigen was demonstrated by testing this antigen with 4 HCV seroconversion panels and a panel of previously tested and stored serum specimens. The artificial antigen was found to specifically detect anti-NS4 antibodies in a number of specimens that were previously found to be anti-NS4 negative. Furthermore, this antigen detected anti-NS4 activity earlier in 2 of 4 seroconversion panels than did the antigen used in a commercially available supplemental assay. Equally important is the observation that the artificial NS4 antigen demonstrated equivalent anti-NS4 immunoreactivity with serum specimens obtained from patients infected with different HCV genotypes, whereas the NS4 recombinant protein derived from genotype 1, used in the commercial supplemental test, was less immunoreactive with serum specimens containing HCV genotypes 2, 3, and 4. Collectively, these data support the significant diagnostic potential of the NS4 mosaic antigen. The strategy employed in this study may be applied to the design and construction of other artificial antigens with improved diagnostically pertinent properties. J. Med. Virol. 59:437-450 1999.

Amino Acid Sequence↗
PubMed1998

Transgenic knockout mice exclusively expressing human hemoglobin S after transfer of a 240-kb betas-globin yeast artificial chromosome: A mouse model of sickle cell anemia.

Sickle cell anemia (SCA) and thalassemia are among the most common genetic diseases worldwide. Current approaches to the development of murine models of SCA involve the elimination of functional murine alpha- and beta-globin genes and substitution with human alpha and betas transgenes. Recently, two groups have produced mice that exclusively express human HbS. The transgenic lines used in these studies were produced by coinjection of human alpha-, gamma-, and beta-globin constructs. Thus, all of the transgenes are integrated at a single chromosomal site. Studies in transgenic mice have demonstrated that the normal gene order and spatial organization of the members of the human beta-globin gene family are required for appropriate developmental and stage-restricted expression of the genes. As the cis-acting sequences that participate in activation and silencing of the gamma- and beta-globin genes are not fully defined, murine models that preserve the normal structure of the locus are likely to have significant advantages for validating future therapies for SCA. To produce a model of SCA that recapitulates not only the phenotype, but also the genotype of patients with SCA, we have generated mice that exclusively express HbS after transfer of a 240-kb betas yeast artificial chromosome. These mice have hemolytic anemia, 10% irreversibly sickled cells in their peripheral blood, reticulocytosis, and other phenotypic features of SCA.

Anemia, Sickle Cell↗
PubMed1998

Laser-induced thermal events in empty and pulp-filled dental pulp chambers.

BACKGROUND AND OBJECTIVE: The purpose of this investigation was to evaluate: 1. thermal events during laser irradiation @2.1 microns of the pulp; 2. whether these effects are adequately modeled using an empty pulp chamber/root canal. STUDY DESIGN/MATERIALS AND METHODS: In extracted human teeth, pulpal access was prepared and thermocouples placed 2, 3 mm apical to the center of the irradiation spot. Pulp-filled or empty pulp chambers were irradiated using a Ho:YAG laser: Spot Size: 1 mm; Power: 1, 2, 3.5, 4.5 W; PRR: 5, 12 Hz; DURATION: 10 sec. Thermal measurements were repeated 3x. RESULTS: Thermal trends did not differ significantly and correlated positively with power (P < 0.01), PRR (P < 0.01), irradiation duration (P < 0.05). No significant difference was determined between temperatures in empty and pulp-filled chambers at all parameters at 5 Hz and at 1-2 W at 12 Hz (P < 0.05, 2-tailed Student's t-test). At 12 Hz and > 3.5 W, pulp chamber temperatures exceeded those in pulpal tissue (P < 0.05). CONCLUSION: Pulp tissues must be present to ensure clinical relevance of thermal measurements.

Animals↗
PubMed1998

Phenoloxidase activity of hemocytes derived from Penaeus monodon and Macrobrachium rosenbergii.

The phenoloxidase (PO) activity of hemocyte lysate supernatant (HLS) from both tiger shrimp (Penaeus monodon) and giant freshwater prawn (Macro-branchium rosenbergii) was examined by treating HLS with various factors, such as an increase in temperatures from 25 to 70 degrees C, one of four elicitors (beta-1,3-1,6-glucan, zymosan, heat-killed Vibrio cells, and lipopolysaccharide), trypsin, one of three protease inhibitors (soybean trypsin inhibitor, p-nitrophenyl-p'-guanidinobenzoate, and benzamidine), and one of two divalent cations (Mg2+ and Ca2+). The strongest PO activity in both animals was induced at 37 degrees C, while enzyme activity varied according to the concentration of the elicitors or cations added to the HLS samples. The following optimum concentrations were recorded: lipopolysaccharides at 0.5 mg/ml, both beta-glucan and zymosan at 1 mg/ml, and Vibrio cells at 10(6) cells/ml. In addition, for giant freshwater prawn, PO activity increased when HLS was treated with trypsin and decreased when it was separately treated with three protease inhibitors. However, effects of either trypsin or protease inhibitors did not occur in tiger shrimp. Strongest PO activity occurred in HLS treated with 20 mM of either calcium ion or magnesium ion, and the addition of the two cations led to an increase in enzyme activity; a decrease was noted following the treatment with EDTA. Cytochemical analysis revealed that prophenoloxidase system exists in the granulocytes of both tiger shrimp and giant freshwater prawn.

Animals↗
PubMed1998

Interaction of Ba2+ with the pores of the cloned inward rectifier K+ channels Kir2.1 expressed in Xenopus oocytes.

Interactions of Ba2+ with K+ and molecules contributing to inward rectification were studied in the cloned inward rectifier K+ channels, Kir2.1. Extracellular Ba2+ blocked Kir2.1 channels with first-order kinetics in a Vm-dependent manner. At Vm more negative than -120 mV, the Kd-Vm relationship became less steep and the dissociation rate constants were larger, suggesting Ba2+ dissociation into the extracellular space. Both depolarization and increasing [K+]i accelerated the recovery from extracellular Ba2+ blockade. Intracellular K+ appears to relieve Ba2+ blockade by competitively slowing the Ba2+ entrance rate, instead of increasing its exit rate by knocking off action. Intracellular spermine (100 microM) reduced, whereas 1 mM [Mg2+]i only slightly reduced, the ability of intracellular K+ to repulse Ba2+ from the channel pore. Intracellular Ba2+ also blocked outward IKir2.1 in a voltage-dependent fashion. At Vm >/= +40 mV, where intrinsic inactivation is prominent, intracellular Ba2+ accelerated the inactivation rate of the outward IKir2.1 in a Vm-independent manner, suggesting interaction of Ba2+ with the intrinsic gate of Kir2.1 channels.

Animals↗
PubMed1998

Tensile bond strengths of dual-cured cements between a glass-ceramic and enamel.

STATEMENT OF THE PROBLEM: Dual-cured cements have been used with castable ceramic restorations, but the tensile bond strengths of these materials have not been thoroughly researched. PURPOSE OF THE STUDY: This study compared the tensile bond strengths between Dicor castable ceramics and enamel of four dual-cure cements: Twinlook, Optec Dual-Cure Luting Cement, Clearfil CR Inlay, and Dual Cement. MATERIAL AND METHODS: Truncated cones made of Dicor castable ceramics were cemented to enamel of freshly extracted anterior teeth with these four cements. Before testing, all specimens were immersed in water at 37 degrees C for 24 hours, and thermocycled 1000 times in 5 degrees C and 55 degrees C water, with a dwell time of 30 seconds each. Tensile force was used to separate each specimen with the Instron universal testing machine. RESULTS: Clearfil CR Inlay cement exhibited the highest mean tensile bond strength (18.4 MPa), followed by Dual (18.3 MPa), Twinlook (15.2 MPa), and Optec Dual-Cure luting (14.9 MPa) cements. One-way analysis of variance did not reveal any significant differences (p = 0.05) among groups. A majority of the fracture was adhesive at the ceramic and cement interface. CONCLUSION: All four dual-cured cements formed strong bonds between enamel and Dicor cement, ranging from 14.90 MPa to 18.35 MPa, and there was no statistically significant difference.

Analysis of Variance↗
PubMed1998

Timed intercourse after intrauterine insemination for treatment of infertility.

OBJECTIVE: To compare the pregnancy rates, between intrauterine insemination (IUI) followed by timed intercourse and IUI only for treatment of the infertile couples. STUDY DESIGN: A prospective study of two different protocols of intrauterine insemination in two hundred and one infertile couples with a normal spermiogram was carried out. Of these, 101 couples were treated with IUI alone and 100 couples had both IUI and timed intercourse within a 12-18 h period. The pregnancy rates were compared between groups. RESULTS: The characteristics of the two groups were similar in terms of the mean age, as well as the duration and causes of infertility. The cycle characteristics following follicular stimulation were also similar between two groups. The pregnancy rate per cycle increased with increasing numbers of total motile sperm per insemination in the IUI alone group (P=0.045). Timed intercourse increased pregnancy rate in patients with lower motile sperm number (<40x10(6)) (27.7% versus 10.5%, P=0.023), but not in patients with higher sperm number (> or =40x10(6)) (25.7% versus 22.7%, P=0.671). CONCLUSIONS: In IUI with low number of motile sperm inseminated, timed intercourse significantly increases the pregnancy rates over IUI alone in infertile couples with a normal sperminogram. This alternative treatment appears to be a practical, simple, and inexpensive addition that improves the pregnancy rate in patients receiving ovulation induction and intrauterine insemination program.

Adult↗
PubMed1998

A review of breast cancer chemoprevention.

Breast cancer remains a major cause of mortality and morbidity, and may be amenable to chemoprevention as estrogen stimulation is believed to be responsible for the promotion of this disease. Tamoxifen is the most widely studied compound for chemoprevention and clinical trials involving over 20,000 women world-wide are currently underway. This drug is well-tolerated with low acute toxicity and high compliance, and has a favorable profile in both decreasing serum cholesterol and increasing bone mineral density in postmenopausal women. However, there are fears of its potential carcinogenicity, especially an increased risk of endometrial cancers, which may jeopardize further recruitment and compliance of women in these chemoprevention studies. Meta-analyses of these studies are expected to be conducted in the year 2000 to address the efficacy of tamoxifen in women with an increased familial predisposition and in those with known germline BRCA mutations.

Anticarcinogenic Agents↗
PubMed1998

Expression and export of Pseudomonas putida NTU-8 creatinase by Escherichia coli using the chitinase signal sequence of Aeromonas hydrophila.

The gene for the creatinase from Pseudomonas putida NTU-8 was sequenced and revealed an open reading frame (ORF) of 1209 base pairs encoding a polypeptide of 403 amino acids with a calculated molecular weight (M(r)) of 45,691. The deduced amino acid sequence is very similar to that of the creatinase of Pseudomonas putida and Flavobacterium sp. An overproduction system for the chitinase signal peptide--creatinase hybrid gene was constructed by using the pQE-51 expression vector in E. coli JM109. The amount of this fusion enzyme was about 50% exported into the periplasmic space of E. coli.

Aeromonas hydrophila↗
PubMed1998

Characterization of emissions of volatile organic compounds from interior alkyd paint.

Alkyd paint continues to be used indoors for application to wood trim, cabinet surfaces, and some kitchen and bathroom walls. Alkyd paint may represent a significant source of volatile organic compounds (VOCs) indoors because of the frequency of use and amount of surface painted. The U.S. Environmental Protection Agency (EPA) is conducting research to characterize VOC emissions from paint and to develop source emission models that can be used for exposure assessment and risk management. The technical approach for this research involves both analysis of the liquid paint to identify and quantify the VOC contents and dynamic small chamber emissions tests to characterize the VOC emissions after application. The predominant constituents of the primer and two alkyd paints selected for testing were straight-chain alkanes (C9-C12); C8-C9 aromatics were minor constituents. Branched chain alkanes were the predominant VOCs in a third paint. A series of tests were performed to evaluate factors that may affect emissions following application of the coatings. The type of substrate (glass, wallboard, or pine board) did not have a substantial impact on the emissions with respect to peak concentrations, the emissions profile, or the amount of VOC mass emitted from the paint. Peak concentrations of total volatile organic compounds (TVOCs) as high as 10,000 mg/m3 were measured during small chamber emissions tests at 0.5 air exchanges per hour (ACH). Over 90% of the VOCs were emitted from the primer and paints during the first 10 hr following application. Emissions were similar from paint applied to bare pine board, a primed board, or a board previously painted with the same paint. The impact of other variable, including film thickness, air velocity at the surface, and air-exchange rate (AER) were consistent with theoretical predictions for gas-phase, mass transfer-controlled emissions. In addition to the alkanes and aromatics, aldehydes were detected in the emissions during paint drying. Hexanal, the predominant aldehyde in the emissions, was not detected in the liquid paint and was apparently an oxidation product formed during drying. This paper summarizes the results of the product analyses and a series of small chamber emissions tests. It also describes the use of a mass balance approach to evaluate the impact of test variables and to assess the quality of the emissions data.

Air Pollutants, Occupational↗
PubMed1998

Population dynamics of the house dust mites Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei (Acari: Pyroglyphidae) at specific relative humidities.

Experiments were conducted to determine the effects of relative humidity on the population dynamics of single and mixed species of Dermatophagoides farinae Hughes, D. pteronyssinus (Trouessart), and Euroglyphus maynei (Cooreman) at specific relative humidities maintained at 20 degrees C, with unlimited food. The population density of single and mixed species (D. farinae and D. pteronyssinus) increased exponentially when cultured at 65, 70, and 75% RH. The mean population growth rates were 17.3 +/- 4.4 SD and 32.5% +/- 4.7/wk for D. farinae and D. pteronyssinus, respectively. Mean population doubling times were 2.2 +/- 0.3 and 4.2 +/- 1.3 wk for D. pteronyssinus and D. farinae, respectively. Mixed species cultures, started with equal numbers of D. farinae and D. pteronyssinus, resulted in higher percentages of D. farinae than D. pteronyssinus. In cultures started with 75% of one species and 25% of the other, the more numerous species remained dominant and in similar ratios throughout the experiment. Both D. farinae and D. pteronyssinus population densities maintained at 85% RH declined over a 12-wk culture period because of mold growth. E. maynei were unable to survive at 65, 70, 75, and 85% RH, which indicated that their climatic requirements were different from those of D. farinae and D. pteronyssinus. Population densities of D. farinae and D. pteronyssinus cultures declined when held at 21-22 degrees C and relative humidities of < or = 50%; however, at 50% RH, significant proportions of the populations survived for 10 wk. Half-life for desiccation of D. farinae and D. pteronyssinus at 45% RH was 11.5 and 1.2 wk, respectively, but at 50% RH was 86.3 and 4.0 wk, respectively. The data indicated that a < or = 50% RH would have to be maintained for long periods to reduce both D. farinae and D. pteronyssinus by desiccation procedures. The results of this study show that D. farinae and D. pteronyssinus have high reproductive potentials and population growth rates, which indicate that mite reduction procedures must be thorough or mite densities will return to high levels quickly following remediation if adequate food and suitable microclimatic conditions exist.

Animals↗