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Biomedical subjects

J C Chan

Publications and source records attributed to J C Chan.

At least 397 records · Page 22Linked to original sources
PubMed1982

The nucleotide sequence of a glutamate tRNA from rat liver.

A glutamate tRNA from rat liver was purified. By means of post-labeling techniques, its nucleotide sequence was shown to be: pU-C-C-C-A-C-A-U-m1G-G-U-C-psi-A-G-C- G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-psi-U-mcm5s2U-U-C-A-C-C-C-A-G-G-C-G- G-C-m5C-m5C-G-G-G-Tm-psi-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH. The sequence is remarkably similar to that of tRNA4Glu from Drosophila melanogaster. Only 10 out of 75 nucleotides in the two tRNAs are different.

Animals↗
PubMed1982

The nucleotide sequence of a glutamine tRNA from rat liver.

A glutamate tRNA from rat liver was purified. By means of post-labeling techniques, its nucleotide sequence was shown to be: pU-C-C-C-A-C-A-U-m1G-G-U-C-psi-A-G-C- G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-psi-U-mcm5S2U-U-C-A-C-C-C-A-G-G-C-G- G-C-m5C-m5C-G-G-G-Tm-psi-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH. The sequence is remarkably similar to that of tRNAGlu from Drosophila melanogaster. Only 10 out of 75 nucleotides in the two tRNAs are different.

Animals↗
PubMed1982

Glomerular filtration rate in children with advanced chronic renal failure: methods of determination and clinical applications.

The rationale, significance and pitfalls of currently available methods for the determination of glomerular filtration rates in children with advanced chronic renal diseases are reviewed. Normal renal clearances in infants and children from birth to adulthood are presented. Methods of serial measurements of renal function, especially by the reciprocals of serum creatinine concentrations, are evaluated. They are applied clinically to monitor the effectiveness of conservative and new modalities of treatment, such as dietary supplementation with essential amino acids plus their keto analogues, and to determine that 1,25-dihydroxyvitamin-D3 treatment of renal osteodystrophy does not accelerate the deterioration of renal function in children with chronic renal insufficiency.

Adolescent↗
PubMed1981

Inhibition of the release of virion-associated murine leukemia virus genomic RNA by vesicular stomatitis virus.

The genomic RNAs of murine leukemia virus (MuLV) and vesicular stomatitis virus (VSV) were distinguishable based on a difference in sedimentation coefficient, polyadenylic acid content, and nucleotide sequence homology. Applying these biochemical characteristics, we found that the release of virion-associated MuLV genomic RNA from virus-producing cells was inhibited by VSV as early as 2 h after superinfection. This finding offers an explanation to our previous inability to detect MuLV(VSV) pseudotype formation during mixed infection with VSV and MuLV.

Adenine↗
PubMed1981

Effects of 1,25-dihydroxyvitamin-D3 on renal function, mineral balance, and growth in children with severe chronic renal failure.

To confirm and extend previous observations of enhanced linear growth in children with chronic renal disease being treated with 1,25-dihydroxyvitamin-D3 and to characterize further the calcium, phosphorus, magnesium, and zinc disorders in renal failure, 11 children (mean age 8 +/- 5 years) with chronic renal insufficiency (glomerular filtration rate 18% +/- 13% of normal) were evaluated on the basis of their reciprocal serum creatinine concentrations, height-velocity curves, mineral balances, and radiologic findings. Reciprocal serum creatinine concentrations analyzed retrospectively and prospectively during 32 months of 1,25-dihydroxyvitamin-D3 therapy showed progression of renal failure at rates linearly identical with those before treatment, thus suggesting that the treatment did not accelerate the rate of deterioration of glomerular filtration rate in chronic anal insufficiency. Indeed, one patient manifested a lesser decline in renal function (P less than .05). The height velocity of six of the children (75%) less than 12 years of age improved markedly over that expected for chronologic and bone ages after one year of treatment with orally administered 1,25-dihydroxyvitamin-D3, 15 to 35 ng/kg/day. All other medications except vitamin D2 were continued at their pretreatment dosage levels throughout the study. Growth velocity was unimproved in two of three children older than 12 years at the initiation of 1,25-dihydroxyvitamin-D3 therapy. Mineral balance data showed significant retention of calcium, phosphorus, magnesium, and zinc (357 +/- 32 mg/sq m/day, 250 +/- 82 mg/sq m/day, 38 +/- 32 mg/sq m/day, and 1,157 +/- 283 microgram/sq m/day, respectively), after treatment for 12 months. In addition, serum calcium, alkaline phosphatase, and parathyroid hormone concentrations returned toward normal. Finally, healing of renal osteodystrophy was radiologically evident after six months of therapy.

Adolescent↗
PubMed1981

A 3-year survey of referral pattern and case material in pediatric nephrology.

A 3-year survey of patient referrals and case material in pediatric nephrology is evaluated to provide a data base for future projection of patient needs. In the 3-year period between January 1978 to December 1980, 538 pediatric patients with renal and electrolyte disorders were evaluated and treated. The principle reasons for the referrals were: hematuria (23%), hypertension (5%), nephrotic syndrome (7%), non-acute glomerulopathies (11%), acute glomerulonephritis (3%), fluid-electrolyte disorders (29%), urinary tract infections (6%), and others. Of the 538 patients, 99 underwent percutaneous renal biopsies under ultrasound guidance. The indications and results of the renal biopsies were also reviewed. The accrual of 18 chronic dialysis patients over a 36-month period is analyzed and presented. The ages of the patients were 4 to 16 years. They weighed from 16 to 51 kg. The primary renal diseases were objective uropathy, chronic glomerulonephritis, membrano-proliferative nephritis, chronic pyelonephritis, focal glomerulonephritis, lupus nephritis and others. All children, except 9 received kidney transplantations. The annual incidence of end-stage renal failure was 4 per million population.

Adolescent↗