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Biomedical subjects

J C Beani

Publications and source records attributed to J C Beani.

50 records · Page 3Linked to original sources
PubMed1981

[Pemphigus vulgaris and Kaposi sarcoma. Report of a new case (author's transl)].

The occurrence of a Kaposi sarcoma during the course of a therapy for a pemphigus vulgaris is reported. Only five such associations have been previously described in the literature. On the other hand, several works have reported simultaneous occurrence of a Kaposi sarcoma with anomalies of the immune or lymphoïd systems (lymphoma, Hodgkin disease, Waldenström disease, mycosis fongoïdes. Hypothesis concerning the Kaposi sarcoma genesis can be suggested on the basis of these observations and remain to be confirmed.

Adult↗
PubMed1980

[Objective evaluation of arterial intermittent claudication by the walking tolerance test. Comparative study of physiological walking and walking on a conveyor belt (author's transl)].

Two methods are used to evaluate the walking distance: physiological walking along a standard path (0% - 6 mk/h) and walking on a tread mill (10% - 3 km/h). In both tests, four data are checked: -- initial trouble distance, -- cramp or walking-distance, -- localisation of pain, -- recovery time. These tests are dependable for the diagnosis of arterial claudication, reproducible and well tolerated. Their results have been compared: there is no correlation between the initial trouble distance and the cramp distance. However there is a correlation between the cramp distance by physiological walking and on treadmill. Recovery time, if long, is a criteria of gravity. Interests of both methods are discussed.

Adult↗
PubMedDate not supplied

Effect of zinc supplementation on resistance of cultured human skin fibroblasts toward oxidant stress.

In purified system zinc has been shown to have an antioxidant role. Its effects on the resistance of cultured cells towards oxidative stress in vitro were examined. Diploid human skin fibroblasts were grown for 21 d in culture media (RPMI 1640 containing 15% fetal calf serum) added with different zinc (Zn) concentrations (100, 125, and 150 microM as Zinc chlorur ZnCl2). In comparison, cell controls were grown in standard culture media (6.5 microM Zn). The intracellular zinc levels of treated fibroblasts increased from 3- to 7-fold (2330 +/- 120 ng/mg protein in 150-microM Zn-treated cells versus 331 +/- 21 ng/mg protein in control cells). The intracellular copper increased 3- fold whereas the iron content slightly but not significantly decreased. The index of basal lipid peroxidation measured as thiobarbituric acid reactants (TBARs) of zinc-supplemented cells was lower than that of non zinc supplemented controls (0.89 mumol/g protein in 150 microM Zn-treated cells versus 1.59 mumol/g protein in controls). At these high doses of zinc, fibroblasts expressed lower antioxidant metalloenzymes activities. Diminished TBARs in Zn treated cells tends to support that Zn acts protectively against free radical mediated damage. However when the cells were challenged with extracellular oxidant stresses mediated by hypoxanthine/xanthine oxidase or hydrogen peroxide (H2O2), an increased toxicity in Zn-supplemented cells was observed. When we applied an intracellular oxidative stress as UV-B or UV-A radiation, Zn-treated fibroblasts were more resistant than cells grown in normal medium. If Zn has shown antioxidant effect in some in vitro or in vivo systems our observations clearly demonstrate that this role is not mediated by antioxidant metalloenzymes.

Cell Survival↗
PubMedDate not supplied

[Drug-induced cutaneous photosensitivity. Results of a consensus meeting].

In France, the use of an official drug adverse reaction assessment method is mandatory since 1984. The method proposes various qualifications for chronologic and semiologic criteria without clear limits. The definitions could vary with the nature of the side-effect. We report here the results of a consensus meeting on drug-induced photosensitivity. Dermatologists and experts in pharmacovigilance studied together how the "French method" could apply to the two variances of drug-induced photosensitivity: phototoxicity and photoallergy.

Drug Eruptions↗
PubMedDate not supplied

[Occlusive rheoplethysmography in the diagnosis, surveillance and evaluation of the sequela of phlebitis].

Occlusive rheoplethysmography (RPO) constitutes a new method of quantitative tetrapolar rheography. It enables us to assess peripheral venous function by measuring distensibility (symbol of influx = IR greater than 0.6, and of drainage = IV greater than 0.7), and flow (symbol of the drainage flow = IDV greater than 0.5). Deep venous thrombosis in the receptor trunk of the popliteal vein and vena cava reduces the IDB (obstructive syndrome). Isolated thrombosis of the tibial vein modifies only the distensibility (restrictive syndrome). RPO is extremely reliable in the diagnosis of acute venous thrombosis of the receptor trunk, as well as in the diagnoses of tibial thromboses, and so it facilitates the recognition of indicants for phlebography. RPO constitutes a simple, portable, rapid, economical and totally objective examination; its use in the observation of the efficiency of treatments as well as in the quantitative assessment of phlebitic after-effects means that it makes a valuable contribution to the management of venous disorders.

Humans↗
PubMedDate not supplied

UV-A1 cytotoxicity and antioxidant defence in keratinocytes and fibroblasts.

The levels of antioxidant molecules and lipid peroxidation, under basal conditions, were measured in normal, human cutaneous keratinocytes and fibroblasts. Total glutathione, glutathione peroxidase and superoxide dismutases are significantly higher in normal keratinocytes compared to normal fibroblasts (respectively +248%, +193% and +155%). Under the same conditions, lipid peroxidation is significantly lower in basal keratinocytes compared to fibroblasts. UV-A1 cytotoxicity was investigated in both cutaneous cell types showing that diploid keratinocytes are more resistant to UV-A1 oxidative stress than fibroblasts (by a factor of around 8). We studied the same parameters in two keratinocyte cell lines, NCTC2544 and HaCaT cells, and in MRC5 fibroblasts. Antioxidant content and lipid peroxidation under basal conditions are quite different in these cell lines compared to those of the normal corresponding cells. Furthermore, NCTC2544 keratinocytes are more sensitive to UV-A1 radiation than normal keratinocytes whereas HaCaT keratinocytes are more resistant, and MRC5 fibroblasts are more resistant than normal cutaneous fibroblasts. These findings suggest that (i) cultured epidermal and dermal cells have different sensitivities to UV-A1 radiation that may be linked to different antioxidant capacities and (ii) cell line response to UV-A1 radiation may differ from that of normal cells.

Analysis of Variance↗