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Biomedical subjects

J Burston

Publications and source records attributed to J Burston.

13 recordsLinked to original sources
PubMed2003

Sidney Dyke.

Explore the source record for details and available documents.

History, 19th Century↗
PubMed1998

TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion.

We previously identified BET3 by its genetic interactions with BET1, a gene whose SNARE-like product acts in endoplasmic reticulum (ER)-to-Golgi transport. To gain insight into the function of Bet3p, we added three c-myc tags to its C-terminus and immunopurified this protein from a clarified detergent extract. Here we report that Bet3p is a member of a large complex ( approximately 800 kDa) that we call TRAPP (transport protein particle). We propose that TRAPP plays a key role in the targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment. The localization of Bet3p to the cis-Golgi complex, as well as biochemical studies showing that Bet3p functions on this compartment, support this hypothesis. TRAPP contains at least nine other constituents, five of which have been identified and shown to be highly conserved novel proteins.

Amino Acid Sequence↗
PubMed1997

Identification and partial characterization of a novel membrane glycoprotein induced by amino acid deprivation in renal epithelial cells.

We have identified a protein of 110 kDa in the renal epithelial cell line NBL-1. which is induced on incubation of the cells in an amino-acid-free medium. The protein was purified on conA-Sepharose and subjected to N-terminal sequencing. The sequence obtained. VDRINFKT, does not correspond to any protein in the databases. Antipeptide antibodies made to this sequence recognised a single protein of 110 kDa in whole cell membranes and in a conconavalin A protein extract. Using the antibody on Western blots, the protein was induced 2.5-3 fold in 10-15 h and the induction was inhibited by cycloheximide and tunicamycin. The protein was found also in rat liver plasma membranes. A procedure for the partial purification of this protein from rat liver is described, and some internal sequence is reported. The possible relationship of the induction of this novel protein to the induction of amino acid transport in these cells by amino acid deprivation is discussed.

Amino Acid Sequence↗