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Biomedical subjects

J Burns

Publications and source records attributed to J Burns.

At least 469 records · Page 26Linked to original sources
PubMed1968

Histochemical investigation of acid mucosubstances in secondary amyloidosis.

Sections of liver and spleen from six cases of secondary amyloidosis have been examined, by various improved histochemical methods, for acidic mucosubstances. The results indicate a complex in amyloid deposits of protein and sulphated mucopolysaccharides in which the principal substance is heparan sulphate with traces of chondroitin sulphates. Deposits also contain sialic acid. The results are compared with chemical analyses of the same tissues and isolated fibrils which confirm the presence of heparan sulphate, chondroitin sulphates, and a sialic acid containing protein.

Acids↗
PubMedDate not supplied

In situ hybridization: an optimised detection protocol for a biotinylated DNA probe renders it more sensitive than a comparable 35S-labelled probe.

The development of a highly sensitive biotinylated in situ hybridization protocol using egg drop syndrome virus infection of domestic fowl as a model is described. This protocol incorporates the use of a monoclonal antibody to biotin as the initial step in a peroxidase-based detection system. The detection of viral nucleic acid in formalin-fixed, paraffin-embedded sections using biotinylated and 35S-labelled probes is compared to the detection of viral antigen by immunocytochemistry. Biotinylated probes detected more positive cells than 35S-labelled probes and were more specific. Biotinylated probes detected more positive cells than immunocytochemistry.

Adenoviridae↗
PubMedDate not supplied

Suppression of the emotional Stroop effect by increased anxiety in patients with social phobia.

Anxious individuals are slower at color-naming threat-related than nonthreat-related words in the emotional Stroop task. Recently, Mathews and Sebastian (1993, Cognition and Emotion, 7, 527-530) reported that this Stroop interference effect disappears when snake-fearful students are exposed to a snake while performing the color-naming task. In the present experiment, we had patients with social phobia and normal control subjects perform an emotional Stroop task under either low anxiety (i.e. upon entering the laboratory) or high anxiety (i.e. before giving a speech). Results indicated that Stroop interference for socially threatening words in the phobic group was suppressed under high anxiety. These findings may indicate that increased effort enables the subjects to suppress the interference produced in the Stroop task.

Adult↗
PubMedDate not supplied

Effects of hyaluronic acid/carboxymethylcellulose gel on bowel anastomoses in the New Zealand white rabbit.

Intra-abdominal adhesions form in more than 90% of patients undergoing major abdominal surgery and can lead to significant complications. Application of a bioresorbable gel consisting of chemically modified hyaluronic acid (HA) and carboxymethylcellulose (CMC) has shown promise as a means of preventing intra-abdominal adhesions, but there have been concerns that the presence of the gel might interfere with the integrity and healing of bowel anastomoses. We tested the effects of HA/CMC gel on adhesion formation and anastomotic healing in 60 New Zealand white rabbits after transection and complete (100%) or incomplete (90%) anastomosis of the ileum. Half of the animals underwent application of HA/CMC gel and half served as control subjects. Animals were killed at 4, 7, or 14 days after surgery. Anastomotic adhesions were scored in a blinded fashion. Integrity of the anastomosis was tested by measuring bursting pressure at the anastomotic site and in an adjacent section of intact bowel. With complete anastomosis, HA/CMC gel significantly reduced adhesion formation at 7 and 14 days after surgery (P<0.05), but gel application did not inhibit adhesion formation when the anastomosis was incomplete. Anastomosed segments of bowel burst at a lower pressure than intact bowel 4 days after surgery, but bursting pressures were normal at 7 and 14 days. Burst pressures of anastomoses receiving an application of HA/CMC gel were nearly identical to control anastomoses at all three time points. HA/CMC gel did not interfere with the normal healing process of bowel anastomoses. Furthermore, HA/CMC gel decreased adhesion formation after complete anastomoses, yet it did not affect adhesion formation in the presence of anastomotic disruption.

Anastomosis, Surgical↗