The dental assistant - a viewpoint.
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Biomedical subjects
Publications and source records attributed to J Brown.
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Mesenteric lymph nodes were collected from healthy sow and slaughter hogs that had been kept in holding pens for an average of 10 and 2 days, respectively, before slaughter at an abattoir. Salmonella was isolated from 58.2% (67/115) of the sows and 31.3% (16/51) of the slaughter hogs. This difference in Salmonella prevalence was significant (chi 2 = 10.22, P less than 0.005); therefore, the hypothesis that there would be no significant difference in the infection rates of sows and slaughter hogs was rejected.
Among the problems encountered today in Expanded Programmes on Immunization (EPI) is the failure to reach an acceptable level of immunization coverage in rapidly growing urban areas. This paper describes a checklist to identify the reasons for such low coverage. The checklist was first used in Yaounde, United Republic of Cameroon. There the low coverage is found to be associated with certain neighborhoods, one ethnic group, low socio-economic status, and newly-arrived families. Publicity about immunizations is not reaching Yaounde residents in those categories. Furthermore, health lessons (given only in French) seem to be confusing the parents rather than educating them. Finally, previous ineffective immunization programmes have made Yaounde residents mistrustful of the current programme. The authors recommend solutions to each of these Yaounde problems. The checklist, and the methods used for answering it in Yaounde, should prove useful to other immunization programmes.
Digestive techniques revealed Sarcocystis sp infection in 62 of 192 (32%) wild swine collected in Alabama, Arkansas, Georgia, Louisiana, Mississippi, North Carolina, South Carolina, Tennessee, Virginia, and West Virginia. Sarcosporidia were not detected in wild swine of Florida and were rarely found in the lower coastal plains of Georgia, Mississippi, and South Carolina. Swine from mountainous terrain in North Carolina, Tennessee, and West Virginia and bottomland floodplain regions of Alabama, Arkansas, and Louisiana were most frequently infected. Infections by sarcocystis sp may have been spread via swine relocated from mountainous regions to some other locales. Upon ingestion of infected pork from wild swine of Tennessee and West Virginia, dogs, but not cats, shed sporocysts morphometrically identical to Sarcocystis suicanis.
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The amino acid sequence of the Salmonella phage P2 repressor and the DNA sequence of its gene c2 have been determined. Sequential Edman degradations on intact P22 repressor and repressor peptides generated by proteolytic and chemical cleavages have been overlapped to give approximately 97% of the complete protein sequence. Additionally, the nucleotide sequence of the P22 c2 repressor gene has been determined by DNA sequencing techniques. The DNA sequence and partial protein sequence are collinear and together define the complete amino acid sequence of P22 repressor. The repressor is a single-chain 216 amino acid polypeptide. Basic residues in the sequence tend to be clustered, and residues 9-20 are highly basic, containing five arginyl and three lysyl residues. The carboxyl-terminal 133 amino acids of the c2 repressor are homologous to the carboxyl-terminal sequence of the coliphage lambda cI repressor. The amino-terminal sequences of these two repressors show little similarity.
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