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Biomedical subjects

J Brodie

Publications and source records attributed to J Brodie.

At least 37 records · Page 2Linked to original sources

Simultaneous multifrequency observations of Markarian 421.

The highly variable BL Lacertae object Mrk 421 has been observed simultaneously in the radio, optical ultraviolet, and X-ray bands over a period of 4 days in early 1984 December and once again in early 1985 January. Using the EXOSAT observatory, we found that dramatic changes occurred in the X-ray flux on a time scale of less than a mouth. During this time the 2-10 keV flux dropped by a factor of 8, whereas the 0.1-1 keV flux decreased by a factor of only 2. These changes were not reproduced at longer wavelengths during the period of simultaneous observations. However, a drop in the ultraviolet flux occurred some months later, which is consistent with the longer characteristic loss times for the lower energy electrons. Since the ultraviolet through radio flux is stable when the X-ray flux is changing, it is extremely unlikely that a simple synchrotron model can account for the full spectrum; in this model the whole spectrum is expected to rise uniformly and in phase as a result of the injection of energetic particles. A simple synchroton self-Compton model that is self-absorbed in the radio also requires an X-ray flux which is many orders of magnitude greater than is observed. However, this discrepancy may be explained by relativistic beaming of electrons with delta > approximately 40 or by a model in which the self-absorption turnover occurs in the optical, and the synchrotron break occurs in the X-rays. Shorter time scale (approximately 10,000 s) variability was also apparent in the 2-10 keV X-ray light curves, and we suggest that it may be a direct measure of the injection time scale. Although reasonable fits resulted when the X-ray data were compared with a simple power-law model with some absorption, a substantial improvement in chi 2 was obtained by adding a high-energy exponential cutoff. Use of this model produced a spectral index close to that typically found in the optical for BL Lacertae objects, in contrast to the high values usually inferred from X-ray spectra.

Astronomical Phenomena↗

Effects of amphetamine on local cerebral metabolism in normal and schizophrenic subjects as determined by positron emission tomography.

The effects of d-amphetamine (0.5 mg/kg PO) on regional cerebral glucose utilization were measured with Positron Emission Tomography (PET). Subjects included ten chronic schizophrenics and six controls who received amphetamine, and six chronic schizophrenics and nine controls who received placebo or no treatment. Amphetamine decreased glucose metabolism in all regions studied (frontal, temporal, and striatal) in normal and schizophrenic subjects. The metabolic effects of amphetamine were correlated with plasma level of the drug. Cortical atrophy was associated with a blunted metabolic response.

Adult↗

Putrescine metabolism in human brain tumors.

The metabolism of the polyamines, putrescine, spermidine and spermine, was studied in human brain and brain tumors. Samples of brain and tumors were incubated with 3H-putrescine and the amounts of labeled polyamines were measured. The amount of putrescine conversion was found to be greater in tumors that in normal brain samples. Furthermore, the metabolism of putrescine in brain tumors was related to tumor type and appeared to correlate with the degree of malignancy. The significance of these findings with regard to positron emission tomographic scanning and therapy of patients with malignant gliomas is discussed.

Astrocytoma↗

Positron emission tomography and computed tomography assessments of the aging human brain.

The relationship between alterations in brain structure and brain function was studied in vivo in both young and elderly human subjects. Computed tomography revealed significant age-related ventricular and cortical sulcal dilatation. The cortical changes were most closely related to age. Positron emission tomography failed to show regional changes in brain glucose metabolic rate. The results suggest that the normal aging brain undergoes structural atrophic changes without incurring regional metabolic changes. Examination of the correlations between the structural and the metabolic measures revealed no significant relationships. These data are discussed with respect to the significant structure-function relationships that have been reported in Alzheimer disease.

Adult↗

Antibodies and the Aberdeen typhoid outbreak of 1964. I. The Widal reaction.

The outbreak of typhoid fever in Aberdeen during 1964 (Walker, 1965) presented an opportunity to study the antibody titres of typhoid fever patients and of TAB immunized individuals to obtain further knowledge concerning the behaviour of these titres with the passage of time.This paper gives an abbreviated version of part of a research programme which followed the Aberdeen typhoid outbreak of 1964.The antibody titres of patients were followed up for a period of 2 years after discharge from hospital and the findings have been compared with those in TAB immunized healthy individuals. The following points emerged:(1) The value of the Widal test as an aid to diagnosis was limited;(2) the flagellar antibody titre in patients' sera provided a more reliable aid towards diagnosis than did the somatic antibody titre;(3) the response of immunized and non-immunized patients to the somatic antigens was poor and often delayed well into the period following discharge from hospital;(4) titres of 1/40 and over for Vi agglutinins were present in immunized and non-immunized patients for at least 12 months after discharge without their being S. typhi excretors;(5) Vi agglutinin titres as high as 1/40 were present in TAB immunized healthy individuals and also in members of the general public;(6) the presence of S. typhi septicaemia need not result in a high antibody titre;(7) patients who relapse, may do so without enhancement of previous antibody titres and may relapse even in the presence of earlier appreciable titres.

Agglutinins↗

Antibodies and the Aberdeen typhoid outbreak of 1964. II. Coombs', complement fixation and fimbrial agglutination tests.

In a previous paper (Brodie, 1977) the value of the Widal test in the diagnosis of typhoid fever was shown to be limited. Evaluation of possible alternative tests showed that: (1) the sensitivity of the anti-human globulin (Coombs') test appeared greater than that of the agglutination test but the length of time (48 h) before results were available rendered it of little value in rapid diagnosis: (2) the complement fixation test offered no particular help towards diagnosis; (3) immunized and non-immunized typhoid fever patients developed fimbrial antibodies, as also did immunized healthy individuals. In this latter group, however, those immunized with alcoholized TAB vaccine had higher antibody titres to fimbrial antigen than those immunized with heat-killed phenolized vaccine.

Agglutination Tests↗

Antibiotics and the Aberdeen typhoid outbreak in 1964.

This paper gives an abbreviated account of part of a research programme which followed the Aberdeen typhoid outbreak in 1964. Chloramphenicol, the main antibiotic used in treatment, was shown to have a minimum inhibitory concentration (MIC) of between 5 and 2-5 mug./ml. for the S. typhi phage type 34 of the outbreak. The MIC for methacycline was between 5 and 2-5 and 2 mug./ml. Whereas the deep and shallow broth techniques used gave similar results with these antibiotics, the MIC for ampicillin, and also cephaloridine, was less in the deep than in the shallow broths. Serum assays in patients given ampicillin or cephaloridine yielded abnormally high concentrations of both antiboitics when S. typhi phage type 34 was the test organism whereas, with other test organisms, the concentrations were within expectation. These abnormally high values fell within expected values when the sera under investigation had first been heated to 56 degrees C. for 30 min. before assay against the S. typhi of the outbreak. The findings with ampicillin suggested that dosages given were satisfactory. With cephaloridine the concentrations found in patients' sera seemed to show that twice daily doses of 0-5 g. fell short of adequacy.

Adult↗

Use of bacteriological investigations by general practitioners.

The numbers of vaginal swabs, faecal specimens, throat swabs, and urine specimens submitted by 104 city general practitioners to the bacteriology laboratory at Aberdeen differed widely. The doctors who made most use of the laboratory service rarely did so equally for all four investigations but usually for only one or two. Similarly, those who used the service least often made frequent use of one particular investigation. Further studies are needed to identify and evaluate the reasons for the apparently substantial disagreement among general practitioners concerning the value of these diffferent bacteriological investigations in general practice.

Bacteriological Techniques↗

Fluid and solid media for isolation of Brucella abortus.

The use of a fluid-enrichment technique increased by 10-16% the recovery rate of Brucella abortus from milks of herds positive to a milk ring test. Three solid media for direct cultural techniques were also tested on the same milks. Combinations of these techniques produced a recovery rate of ca. 88% from MRT-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the MRT cream layer as inoculum. B. abortus biotypes 1-5 and 9 were isolated and growth of all the biotypes was supported by all media used.

Animals↗

Pregnancy testing.

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Costs and Cost Analysis↗

Effect of trimethoprim-sulphamethoxazole on typhoid and salmonella carriers.

Four chronic typhoid carriers from the Aberdeen typhoid outbreak of 1964 were treated with trimethoprim-sulphamethoxazole. The male carrier remained clear of infection following treatment but the three female carriers, all with gall bladder abnormalities, remained excretors. A convalescent typhoid excretor also remained clear of infection after treatment as well as two other salmonella carriers, one of whom has been a chronic carrier for 24 years.

Adult↗