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Biomedical subjects

J Bramley

Publications and source records attributed to J Bramley.

6 recordsLinked to original sources
PubMed1995

A serum-sensitive, sialyltransferase-deficient mutant of Neisseria gonorrhoeae defective in conversion to serum resistance by CMP-NANA or blood cell extracts.

A stable, sialyltransferase-deficient mutant of Neisseria gonorrhoeae strain F62 totally defective in CMP-NANA-dependent lipopolysaccharide (LPS) sialylation was isolated by insertion mutagenesis with transposon Tn 1545-delta 3 and screened for unlabelled colonies following incubation with CMP-14C-NANA. In contrast to the parental strain which became serum resistant on incubation with CMP-NANA or blood cell extracts, the mutant, JB1, remained serum sensitive. French press extracts of strain F62 catalysed LPS sialylation, but corresponding extracts of the mutant were inactive. Five LPS components were detected by SDS-PAGE in the parental strain. Five components of the same Mr were also found in the mutant. Three identical components were detected by Western blotting using MAb 3F11, which recognizes the Gal beta 1-4GlcNAc groups in the conserved LPS components of F62 which can be sialylated. The mutant, JB1, is therefore deficient in the sialyltransferase that is essential for both LPS sialylation and conversion of serum-sensitive gonococci to serum resistance by either CMP-NANA or blood cell extracts. No evidence was obtained for an LPS sialylation pathway by blood cell extracts that is independent of CMP-NANA.

Antibodies, Monoclonal↗
PubMed1995

Employment, retirement and elderly persons with an intellectual disability.

A national Australian study of people with an intellectual disability of 55 years of age and over investigated their employment and retirement patterns, attitudes to work and retirement, and the degree to which they were involved in leisure or recreational programmes. Two cohorts were recruited: one included all known members of the target group who agreed to participate in the study in the states of Queensland and Western Australia; and the second was a proportional, random sample drawn from a national database on a state population basis. A large number of the participants had never been involved in full- or part-time employment, either in a competitive or supported environment, or in workshops. The majority of those who had worked expressed strong positive attitudes toward employment and concern about retirement, suggesting the need for pre-retirement programmes including transition and choice-making skills to prepare participants for the future.

Aged↗
PubMed1995

Residential circumstances of older Australians with mental retardation.

Residential circumstances of Australians 55 years or older with mental retardation were investigated. Data were collected via interviews from 446 individuals living in Queensland and Western Australia who were identified following an extensive national search. Although survey results indicated that older people lived in a wide variety of residential settings, the majority still resided in large private or public institutions. There is evidence that deinstitutionalization had occurred, although it affected only 35% of the cohort. One important finding in the study concerns the relatively minor role participants play in determining their future care and residential needs. Interviews established that less than 10% of those in each sample would be involved in future decision-making processes.

Aged↗
PubMed1983

Coliform mastitis.

The majority of coliform mastitis cases are relatively mild and self-limiting. Teat dipping and dry cow therapy will not prevent coliform problems, neither do they cause them. Bad housing and poor milking time hygiene lead to coliform mastitis. Efforts to control coliform mastitis must be directed at reducing exposure to the bacteria. In acute cases fluid therapy is indicated to counteract endotoxic shock.

Animals↗