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Biomedical subjects

J Brachet

Publications and source records attributed to J Brachet.

At least 91 records · Page 5Linked to original sources
PubMed1969

Gene expression in interspecific hybrids. I. DNA synthesis in the lethal cross Arbacia lixula male X Paracentrotus lividus female.

The technique of molecular hybridization has been used to determine in what proportion the DNA's of paternal and maternal origin are present in the lethal hybrid obtained by fertilizing the eggs of the sea urchin Paracentrotus lividus with the sperm of another sea urchin species, Arbacia lixula. The ability of the hybridization technique to recognize DNA molecules from A. lixula and P. lividus was examined and proved to be satisfactory. Labeled DNA's from A. lixula and P. lividus were found to hybridize respectively ten and seven times as well with DNA of their own species as with DNA of the other species. Labeled DNA from arrested hybrid embryos hybridized 1.7 times to twice as well with P. lividus as with A. lixula DNA. We conclude that the interspecific hybrid contains about 2.5 times as much DNA of maternal origin as DNA of paternal origin. The lower content of A. lixula DNA probably results from a partial elimination of the paternal chromosomes during the late cleavage stages.

Animals↗
PubMed1969

Gene expression in interspecific hybrids. II. RNA synthesis in the lethal cross Arbacia lixula male x Paracentrotus lividus female.

All types of RNA are actively synthesized by the lethal hybrid obtained by fertilizing the eggs of the sea urchin Paracentrotus lividus with the sperm of another sea urchin species, Arbacia lixula. DNA-like RNA and ribosomal RNA seem to be less stable in hybrids than in normal A. lixula and P. lividus embryos. Labeled RNA's from A. lixula and P. lividus were found to hybridize about 2.5 times as well with DNA of their own species as with DNA of the other species. RNA from the interspecific hybrid hybridized from 1.6 times to twice as well with A. lixula DNA as with P. lividus DNA, although the hybrid embryos were previously shown(5) to contain more DNA of maternal than of paternal type. Transcription of the paternal (foreign) genome seems therefore to be favored in some way in the interspecific hybrid.

Chromatography↗