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Biomedical subjects

J Bosq

Publications and source records attributed to J Bosq.

90 records · Page 5Linked to original sources
PubMed1989

[Immunohistochemical study of 20 synovial sarcomas in children].

Histopathologic study of 20 cases of synovial sarcoma (S.S.) in children has been conducted to evaluate the usefulness of immunohistochemistry in the diagnostic assessment of this tumor. Two of the 6 antibodies studied have been found to be the most informative: staining for keratin and vimentin proteins has been observed in most cases, outlining the epithelial and sarcomatous components of this tumor. The results are in accordance with those already described for S.S. in adults. The presence of cytokeratins in S.S. is discordant with classical histogenetic conceptions.

Child↗
PubMed1987

Expression of a Hodgkin and Reed-Sternberg cell associated antigen (Ki-1) in cutaneous lymphoid infiltrates.

Skin biopsy specimens from normal skin and from 115 patients with benign dermatoses, pre- or pseudo-malignant disorders or malignant cutaneous lymphomas have been examined immunohistologically for expression of the Reed-Sternberg cell associated antigen CD30 detected by monoclonal antibodies Ki-1 and Ber-H2. The antibodies stained the atypical cells in lymphomatoid papulosis, a proportion of the neoplastic cells in some cases of mycosis fungoides and most of the neoplastic cells in six large cell anaplastic/pleomorphic non-Hodgkin's lymphomas. The lymphoid cells in all other specimens were Ki-1- and Ber-H2-negative. In all cases, expression of the Ki-1/Ber-H2 antigen was accompanied by expression of activation and proliferation associated markers (i.e., HLA-DR, IL-2 receptor, transferrin receptor and the Ki-67 nuclear antigen). These data indicate the value of antibodies Ki-1 and Ber-H2 in distinguishing between lymphomatoid papulosis and other types of pre- or pseudo-malignant disorders and support the view that lymphomatoid papulosis, Hodgkin's disease and some types of non-Hodgkin's lymphoma constitute a spectrum of related disorders, originated from activated lymphoid cells.

Antibodies, Monoclonal↗
PubMed1987

[Non-Hodgkin's malignant lymphomas of the nasopharynx. Apropos of 24 cases treated at the Institut Gustave-Roussy].

Twenty-four patients with non Hodgkin's lymphoma of nasopharynx were treated in the Institut Gustave-Roussy, France, between 1976 and 1983. Mean age of patients was 53 years and the male/female sex ratio was 2. Symptomatology was immediately suggestive of a lesion in the nasopharynx in 21 of the 24 patients, and histology gave an unfavorable prognosis (diffuse large cell tumors) in 71% of cases. Complete investigations showed the lymphoma to be relatively limited in extent in the majority of cases (68% of stages I and II). These findings are in agreement with published reports. The 4 patient with stage I disease were treated by irradiation alone and are all alive without recurrence after 43 months. The 11 patients with stage II lesions received combined chemo-radiotherapy, and at 42-month follow up 8 were alive without recurrence (1 patient died of intercurrent illness). Results of conventional chemotherapy (CHVmP) in patients with stages III and IV were disappointing, however: only one patient survived for 60 months (after a cerebral recurrence treated by irradiation). Although current therapy is effective for stage I and II lesions, patients with stages III and IV require more aggressive therapy recently developed: heavy multiple chemotherapy, and even intensive chemotherapy-total body irradiation and bone marrow graft.

Adult↗
PubMed1986

Expression of a cell-cycle-associated nuclear antigen (Ki-67) in cutaneous lymphoid infiltrates.

Cryostat sections of skin biopsies from 83 patients with benign or malignant cutaneous lymphoid infiltrates were examined immunohistologically for reactivity with Ki-67 (a monoclonal antibody recognizing a nuclear antigen expressed by cycling cells) using the APAAP immunoalkaline phosphatase labeling method and a double immunoperoxidase/alkaline phosphatase staining technique. Ki-67-positive neoplastic lymphocytes were plentiful in all large-cell lymphoma cases and in one-third of the small-cell lymphomas. Some benign disorders (patch test biopsies, cutaneous lymphocytoma, lichen planus) also contained many Ki-67-positive lymphocytes. These data indicate the value of using Ki-67 to assess the proliferative capacities of the lymphoid cells in cutaneous infiltrates. Use of this technique in the future may have important prognostic and therapeutic implications in the management of cutaneous lymphoid malignancies.

Antibodies, Monoclonal↗
PubMed1986

Immunocytochemical characterisation of cutaneous lymphomas other than mycosis fungoides.

The immunophenotypic properties of 25 cutaneous non-Hodgkin lymphomas other than mycosis fungoides or Sezary syndrome were investigated and correlated with clinical and histopathological data. The 11 low grade lymphomas were all of B cell origin, whereas the 14 high grade lymphomas comprised B and T cell tumours, true histiocytic proliferations, and one "nul" cell lymphoid neoplasm. For the high grade lymphomas correct prediction of the immunological phenotype based on morphological criteria was only possible in three cases. In contrast, all of the low grade lymphomas showed the non-epidermotropic infiltration pattern considered to be characteristic of cutaneous B cell tumours. For these conditions, however, immunophenotypic investigations provided a convenient means of improving discrimination between benign (polyclonal) and malignant (monoclonal) lesions, and also showed similarities with nodal lymphomas in terms of expression of lymphoid subset markers and composition of the non-neoplastic white cell infiltrate. No differences were identified between primary and secondary or concurrent cutaneous and extracutaneous lymphomas. Cutaneous non-Hodgkin lymphomas other than mycosis fungoides or Sezary syndrome constitute a heterogeneous group of neoplasms and most of these disorders are likely to represent cutaneous equivalents of nodal malignancies. Immunophenotypic investigations form a useful supplement to their histogenetic characterisation and may provide a common conceptual basis for their classification.

Adolescent↗
PubMed1986

[Immunohistologic marking of epithelial antigens in sarcomatoid carcinomas of the upper aerodigestive tracts].

The immunoperoxidase localization of epithelial antigen (keratin, epithelial membrane antigen) was identified in 18 cases of sarcomatoid carcinomas localized in the upper aero-digestive tract. These tumors are an association of a differentiated squamous cell carcinomatous component and of spindle, or pleomorphic cells component. The anti-keratin monoclonal antibody KL1 stained the sarcomatoid component in 4 cases. A continuous staining between differentiated squamous cells and spindle cells in observed in two cases. The identification of cytokeratin is better with KL1 than with the polyclonal anti-keratin antiserum KT. The monoclonal antibody HMFG2 is not retained. These results suggest the dedifferentiation of epithelial cells in sarcomatoid component. They confirm the heterogeneity of these tumors, which has been previously found in ultrastructural studies. These immunostaining are helpful in recognizing sarcomatoid carcinoma, when they are only represented by the sarcomatoid component.

Aged↗
PubMed1986

[Histo-prognostic classification of undifferentiated carcinoma of nasopharyngeal type. Importance of the study of the stroma].

A total of 102 cases of undifferentiated carcinoma of nasopharyngeal type (UCNT) were reviewed to attempt their classification into histologic forms of different prognosis. Each slide reviewed was evaluated for predominant tumoral tissue architecture, and the quality, amount and topography of the stroma. Immunoperoxidase studies in 80 cases allowed definition of the immunologic profile of the lymphoplasmocytic stroma. For equivalent clinical stages, the different histologic types are defined as a function of the architecture: cordonal or diffuse, compact or fragmented; these forms, when combined with cytologic characteristics and quality of stroma distinguished 3 microscopic groups of different evolution. The fragmented diffuse forms appear to have the best prognosis (85.5% survival at 5 years), the compact cordonal forms the worse prognosis (13.2% survival at 5 years): the intermediate or diffuse compact forms were of fair prognosis (42.8% survival at 5 years). Application of histology to determine prognosis of UCNT should allow future adaptation of treatment (radiotherapy and/or chemotherapy) to each case.

Adolescent↗
PubMed1985

Role of immunohistochemistry in diagnosis of nasopharyngeal tumours.

To evaluate the usefulness of immunocytochemistry in the differential diagnosis of nasopharyngeal tumours, 35 undifferentiated nasal carcinomas were examined with a panel of monoclonal antibodies against a wide variety of epithelial and non-epithelial antigens. The results were compared with those obtained from a series of nasopharyngeal tumours comprising three squamous cell carcinomas, six lymphomas, one rhabdomyosarcoma, and one yolk sac tumour. All of the carcinomas were positive with at least one of the antiepithelial markers and negative for the leucocyte common antigen and were clearly distinguishable from the nasopharyngeal lymphomas, which gave the opposite staining pattern. It was concluded that such a panel of monoclonal antibodies would be extremely useful for the differential diagnosis of nasopharyngeal tumours, particularly with small or technically inadequate biopsies.

Antibodies, Monoclonal↗
PubMed1984

HNK-1-defined antigen detected in paraffin-embedded neuroectoderm tumors and those derived from cells of the amine precursor uptake and decarboxylation system.

The reactivity of the HNK-1 monoclonal antibody that, besides a subset of hematopoietic cells with natural killer activity, also detects neuroectodermal cells, was screened on 153 human tumors. Immunoperoxidase staining of paraffin sections revealed that HNK-1 detects neuroectoderm-derived cancers and tumors with endocrine activity. Because the antigen detected by HNK-1 is well conserved in paraffin sections, this antibody is of potential diagnostic value in routine histopathology and might help to elucidate the histogenesis of some poorly understood tumors.

Adult↗
PubMed1983

Superficial extending carcinoma of the hypopharynx: report of 26 cases of an underestimated carcinoma.

Advanced ulcerating and infiltration tumors are commonly found in the hypopharynx, whereas early well-defined lesions are rarely diagnosed. The pathologic reports of 242 uniformly studied surgical specimens after total pharyngolaryngectomy for cancer of the hypopharynx were reviewed. The histologic analysis of 26 cancers (10.7%), which were recorded as having an entire or predominant superficial type of spreading, demonstrated that also in the hypopharynx a "superficial extending carcinoma" (SEC) may occur. SEC of hypopharynx was pathologically defined as a poorly or moderately differentiated squamous cell carcinoma, generally located in the pyriform sinus, which spreads superficially. It was limited to the mucosa (2.9%), but more frequently early infiltrated the underlying muscle or gland structures (6.2%), regardless of the presence of lymph node metastases or lymph vessels invasion. Although the concept that SEC of the hypopharynx may be an expression of a generalized disease of the mucosa must be carefully considered in surgical management, it appeared that this carcinoma in its "pure" intramucosal form may be associated with a good prognosis and a long survival.

Adult↗
PubMed1983

[Tumor regression following local chemotherapy in carcinomas of the upper respiratory and digestive tracts. The viewpoint of the pathologist].

Few pathologists have given much thought to the problem of post-chemotherapeutic tumoral regression of upper respiratory and digestive tract carcinoma. In other oncological fields (pediatry: nephroblastoma, embryonic sarcoma, osteosarcoma; gynecology: mammary tumors...) pre- and post-chemotherapy tissue studies have led to histological grading of prognostic value (particularly well known is that of Rosen et al). Numerous specimens were examined following operations for cancer, including pelvimandibulectomy, buccopharyngectomy, facial resection... Possible tumoral changes were evaluated using a conventional scale of regression based on 6 histologic degrees, and findings were of prognostic significance when compared with the initial biopsy results. This evaluation scale may require modification in the near future since recently introduced chemotherapy, particularly cisplatinum, appears to have a more potent and more specific effect on histologie features.

Carcinoma↗
PubMedDate not supplied

Squamous cell carcinoma of the pyriform sinus: retrospective study of 351 cases treated at the Institut Gustave-Roussy.

The first part of the study was devoted to 199 tumors treated by surgery, either conservative for the smallest tumors (18 cases) or radical (181 cases), with systematic postoperative radiotherapy. The 3-year survival rate was 48% and the 5-year, 33%, with a 12% local recurrence rate, a 7.5% neck recurrence rate, and 27.6% rate distant metastases. Histologic correlations were developed. The second part of the study reported 152 cases treated by external radiotherapy alone either as a variant of our treatment protocol for the small-sized tumors (31 cases) or, for the major part (121 cases), as a result of surgical inoperability or patient refusal. The former subgroup had a variable survival rate (65% at 3 years and 40% at 5 years) equivalent to similarly staged patients treated with conservation laryngeal surgery, whereas the prognosis of the latter subgroup was poor. The two main causes of failure were the inability to apply the curative treatment protocol in 35% of patients ineligible for a surgery and the high risk of distant metastases in the 65% of patients able to undergo the usual management.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Potential role for low dose limited-field radiation therapy (2 x 2 grays) in advanced low-grade non-Hodgkin's lymphomas.

The purpose of this retrospective study was to evaluate the efficacy of low-dose limited field radiation therapy (LDLRT) in low-grade non-Hodgkin's lymphoma (NHL) patients, in order to outline its possible role. The rationale of the analysis was as follows: (1) there is no clear dose-response relationship for radiation therapy (RT) in localized low-grade non-Hodgkin's lymphomas (LGNHL); (2) long-term disease-free survival can be achieved in advanced LGNHL with low-dose (1.5-2 Gy) fractionated total body irradiation; (3) moreover, some of our previous LGNHL patients stopped their conventional planned RT course for convenience after 5 to 7.5 Gy and remained free from local progression in irradiated volumes for a long period of time. Patient selection criteria were the following: (1) patients with low-grade NHL (LGNHL); (2) patients who received no other form of therapy concomitantly with LDLRT until evaluation of the response; (3) patients who received a planned dose of 4 Gy in two fractions and over 3 days in a limited field. Out of 37 patients, 27 patients were fully evaluable. Twenty-two patients had stage III or IV disease. The median longevity of the disease was 73 months. Twenty-five patients had previously received chemotherapy (18 with anthracyclines). Nineteen patients had received one LDLRT course only. Eight patients responding to the first LDLRT had received at least one subsequent LDLRT course. After the first LDLRT course, an objective response in irradiated sites was observed in 24 of the 27 patients (89 per cent). Ten and 14 patients respectively demonstrated a complete response (CR) (37 per cent) and partial response (PR) (52 per cent). Freedom from progression in irradiated volumes for evaluable patients ranged from 4 to 35 months. Among the eight patients who received at least two LDLRT courses, a total of 20 different areas were irradiated, and 15 areas (75 per cent) showed a CR. Toxicity due to LDLRT was minimal. In conclusion, low-dose limited-field RT resulted in a high proportion of responses in LGNHL. The mechanisms explaining this radioresponsiveness are poorly understood. However, the efficacy of LDLRT could have several clinical applications in the general management of patients with advanced LGNHL.

Adult↗
PubMedDate not supplied

Synovial sarcomas of the head and neck: CT and MR findings.

The authors present three cases of histologically proved synovial sarcoma. CT is useful in assessing erosive or destructive changes in bone, and in demonstrating calcifications. MR characteristics are nonspecific; calcifications within these tumors can be missed on MR. MR can help to assess tumor extension, vascular invasion, and hemorrhage within the tumor.

Adolescent↗