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Biomedical subjects

J Bonal

Publications and source records attributed to J Bonal.

79 records · Page 5Linked to original sources
PubMedDate not supplied

Microbiological quality control study of "all-in-one" total parenteral nutrition admixtures.

A microbiological quality control study of parenteral nutrition (PN) is presented. The study was carried out in the Pharmacy and Microbiology Services of the Hospital de la Santa Creu i Sant Pau (Barcelona, Spain) from April 1991 to May 1998 and represents more than 25,000 lipid-containing PN admixtures prepared during that 7-year period. A randomized sampling procedure according to the cumulative sum control charts was performed and a 0.45-microm membrane filtration method was used as the microbiological test. Validation showed that the method is sensitive for sterility tests. Bacterial growth was observed on 59 filters (4.58% of the samples). A second control was made for these positive outcomes, and bacterial growth was found in only two cases. The patients' clinical history was reviewed and no relation between the microorganisms of the nutrition and the results obtained from blood cultures was observed. The quality control study revealed a low incidence of contamination in our PN preparation process.

Bacteria↗
PubMedDate not supplied

Liposomes as carriers of different new lipophilic antitumour drugs: a preliminary report.

We studied the liposome formulation characteristics of eight new lipophilic antitumour agents that have demonstrated a broad spectrum of antitumour activity in preclinical in vitro and in vivo screening models. Multilamellar vesicles were prepared by using standard evaporation/hydration methods. The drug to lipid weight ratio was 1:15 in all cases. Different combinations of DMPC, DMPG and cholesterol were used. The quality of the liposomal formulations was evaluated by calculating the percentage drug bound to the liposome phase and assessing the morphology of the liposome phase by optic microscopy, to rule out the presence of drug crystals or drug/lipid microaggregates. Good liposomal preparations were obtained with hexamethylmelamine, penclomedine, mitindomide, and fazarabine. However, with taxol, batracylin, trimelamol, and diaziquone, the presence of crystals of free drug or microaggregates of lipid/drug complex was observed in all preparations, independently of lipid composition. In general, mixtures of DMPC:DMPG at a molar ratio between 7:3 and 9:1, and the addition of 5 per cent cholesterol (w/w) gave the optimal results. In vitro cytotoxicity studies of free and liposomal drugs against L1210 cells showed changes in both directions after liposome entrapment, thus suggesting that liposome entrapment may alter drug cellular uptake or may result in chemical modifications of the entrapped drug. Because of the limited number of compounds studied we were unable to identify general chemical characteristics required for an enhanced liposome formation and drug entrapment.

Animals↗
PubMedDate not supplied

[Hepatic tuberculosis in a patient with diabetic nephropathy. An unusual clinical presentation].

Hepatic tuberculosis is an exceptional form of presentation of extrapulmonary tuberculosis, but in the last years a greater incidence has been found in patients with HIV infection and/or consumptive diseases or immunosuppressive treatment. For this condition, the diagnosis of hepatic tuberculosis in other population groups represents a true clinical challenge because of the scarce specificity of both the clinical and biological manifestations. Despite deferral of the diagnosis, fast improvement is observed in the clinical symptomatology in most cases following initiation of tuberculostatic treatment.

Diabetic Nephropathies↗
PubMedDate not supplied

[Determination of nutritional parameters affected in patients with gastric adenocarcinoma who are candidates for surgery according to tumor stage].

An attempt is made to determine the nutritional parameters which are affected in patients with a gastric adeno-carcinoma, who are candidates for surgery, and to relate these parameters to the surgical technique employed, and to the tumor stage. Later, they aim to determine the parameters which may be indicative of the surgical prognosis of these patients. To this end, between January 1992 and October 1994, 66 patients, distributed in two groups, were studied. Group A included the patients who had undergone a total gastrectomy or a partial gastrectomy, and group B included those individuals subjected to an exploratory laparotomy, gastro-intestinal derivation, and subtotal or palliative gastrectomy. When analyzing the results obtained in the two groups of patients, it is observed that all of them have one or more altered nutritional parameters, and that the means of these parameters are more altered in group B than in group A. However, only the percentage of weight loss and the serum albumin level present significant differences between the two groups. That is to say, we observe that patients with serum albumin levels lower than 35 g/l, and with a weight loss of > 6%, have a greater risk of being subjected to an exploratory laparotomy, a gastro-intestinal derivation, or a total or subtotal palliative gastrectomy, than do patients with serum albumin levels > 35 g/l and a weight loss of < or = 6%. We may conclude that the loss of weight and the serum albumin level may help to decide a more or less aggressive surgical approach, and to predict the possible resectability of the tumor.

Adenocarcinoma↗