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Biomedical subjects

J Black

Publications and source records attributed to J Black.

At least 235 records · Page 13Linked to original sources

Co-morbidity: lessons learned about post-traumatic stress disorder (PTSD) from developing PTSD scales for the MMPI.

Results from efforts to develop and validate PTSD measures are promising, but a "gold standard" has not been achieved. Keane, Malloy, and Fairbank (1984) have developed an MMPI PTSD subscale that has been cross-validated with clinicians' classification of PTSD at acceptable levels of agreement, specificity, and sensitivity. There is, however, room for improvement. Empirical evidence is presented that indicates that the next round of efforts to increase reliability and validity of PTSD measures must account for the presence/absence of co-morbidity (i.e., the simultaneous occurrence of other psychiatric disorders). For example, differences are noted in MMPI group profiles and PTSD scales between psychiatric patients and substance abusers. Second, different MMPI items emerge as indicative of PTSD; these vary as a function of the presence of other Axis I disorders among groups of Vietnam combat veterans who seek treatment for substance abuse. Results substantiate that different MMPI items for classifying PTSD occur with groups that differ in co-morbidity. Improvements in PTSD scale development are more likely when the contributions of pre-existing or subsequently co-occurring psychiatric disorders are taken in account, as well as variations in level of personality maturity. The evidence suggests that a "family" of PTSD scales need to be developed that take into account co-morbidity differences.

Adaptation, Psychological↗

Ethnicity: post-traumatic stress disorder (PTSD) differences among black, white, and Hispanic veterans who differ in degrees of exposure to combat in Vietnam.

Clinical observations and empirical evidence suggest that, among Vietnam combat veterans, Blacks are more maladjusted than Whites (e.g., Parsons, 1985; Penk et al., 1985). The prediction that minority group status is associated with poorer post-war adjustment and higher rates of PTSD was examined among Vietnam combat veterans who were seeking treatment for addiction disorders. Adjustment scores among groups comparable in combat exposure were found to be similar for both Whites and Hispanics; Blacks, however, score significantly higher on both PTSD symptoms on MMPI scales. These findings indicate that ethnicity contributes importantly to PTSD in selected instances, but that minority group status alone does not account for observed differences. Additional research is indicated in which careful attention is given to the complicating and interacting role of addiction disorders in sampling.

Adaptation, Psychological↗

"Push-out" tests.

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Data Collection↗

Short-term primary culture of rat prostate tumor epithelial cells on reconstituted basement membrane as a useful in vitro model to assess drug action on prostatic cancer: efficacy of the thiazolidinedione derivative CGP 19984.

A model system has been developed that permits short-term culture of rat R3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. Growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. This model system was used to test the efficacy of the thiazolidinedione derivative CGP 19984, a drug that inhibits luteinizing hormone secretion in vivo. At concentrations ranging between 1 and 25 micrograms/ml, CGP 19984 inhibited growth of the prostate tumor epithelial cells in a dose-dependent manner. The results thus demonstrate a direct effect of CGP 19984, which complements its indirect antitumor action in vivo, and suggest that this drug might be an effective agent for treatment of prostatic cancer. Moreover, growth of prostate tumor epithelial cells on a reconstituted basement membrane provides a useful system for in vitro testing of drugs for prostate cancer.

Adenocarcinoma↗

Effects of botulinum neurotoxin and Lambert-Eaton myasthenic syndrome IgG at mouse nerve terminals.

The interaction between two presynaptically acting agents, Lambert-Eaton myasthenic syndrome (LEMS) immunoglobulin G (IgG) and purified botulinum neurotoxin (BoNT) type A, was studied. Intracellular microelectrode recordings were carried out on mouse muscles after injection with LEMS IgG. BoNT was either injected before recordings were made or applied in vitro. The time course of the in vitro actions of BoNT on miniature end-plate potential and end-plate potential parameters were not affected by pretreatment with LEMS IgG. After in vivo injection of BoNT, end-plate potential quantal content was reduced to less than 2% of control values, whether or not LEMS IgG had also been previously given. Quantitative electron-microscope autoradiographical analysis showed that neither the binding of 125I-BoNT to acceptors on the nerve terminal membrane nor the pattern of its internalisation were affected by pretreatment with LEMS IgG. We conclude that the effects of BoNT are not affected by LEMS IgG, suggesting different presynaptic binding sites for the two agents.

Action Potentials↗

Interactions between chloramphenicol, acepromazine, phenylbutazone, rifampin and thiamylal in the horse.

The potential for interactions between chloramphenicol, phenylbutazone, acepromazine and thiamylal and chloramphenicol, rifampin, and phenylbutazone were evaluated in two groups of experiments. In the first, five horses were given thiamylal intravenously (iv) (6.6 mg/kg) after pretreatment with acepromazine, and the time of recumbency was determined. Administration of chloramphenicol iv (25 mg/kg) 1 h prior to anaesthesia significantly lengthened the recumbency time from 21.8 +/- 4.8 mins to 36.0 +/- 8.3 mins. There was an apparent but not statistically significant decrease in recumbency time when phenylbutazone (4.4 mg/kg) was administered iv daily for 4 days prior to anaesthesia. In the second series of experiments, phenylbutazone (4.4 mg/kg), chloramphenicol (25 mg/kg) and rifampin (10 mg/kg) were administered in various sequences to five different horses. Chloramphenicol pretreatment produced a significant decrease in the elimination rate and rifampin a significant increase in the elimination rate of phenylbutazone. The half-life of elimination of phenylbutazone alone was about 4 h. Following four days pretreatment with rifampin it was approximately 2.7 h, it was approximately 5.6 h and 9.5 h, respectively, when chloramphenicol was administered in one dose 1 h before or two doses 12 h and 1 h before phenylbutazone.

Acepromazine↗

Requirements for successful total knee replacement. Material considerations.

Despite the overall clinical success of TKR arthroplasty, materials' limitations are becoming increasingly obvious as designs and surgical techniques improve. As in the case of THR components, the ultimate limiting factor now appears to be wear of the tibiofemoral articulation and its biologic consequences. Although some materials appear to hold out promise of improvement, no combination better than cobalt-base alloy/UHMWPE has yet appeared. In addition, the very long service times now expected routinely (greater than 10 years) for total joint replacement increase the problem of new materials' selection owing to the well-known disparities between in vitro wear tests and in vivo performance and the absence of any consensus on correlation between accelerated testing and real-time performance, either in vitro or in vivo. For these reasons, extreme caution is counseled in the early clinical trials of newer materials and combinations of materials. The clinical "rule of thumb" for publication of clinical results, 2-year minimum follow-up, is necessary but highly insufficient for materials' qualification. Good to excellent results, with no signs of progressive failure for 5 to 7 years of prospective study, combined with high-yield follow-up to 10 years, will probably be necessary for safe introduction of such materials into general clinical use. An excessively rapid introduction could predispose to clinical failure, through lack of knowledge of critical design and manufacturing parameters, and prove an impediment to improvement of TKR arthroplasty.

Humans↗

Direct extracellular electrical stimulation influences density dependent aggregation of fetal rat cerebrocortical neurons in vitro.

Cells isolated from fetal (E17) rat cerebral cortex form aggregates in a density-dependent manner. Direct current electrical stimulation influences the formation of neuronal aggregates by promoting neuronal survival at a calculated current density of 15 nA/cm2 (with an average calculated field strength of 1 microV/cm), and inhibiting neuronal survival at calculated current densities of both 1 nA/cm2 and 150 nA/cm2. Electrical stimulation had no observable effect on neuronal aggregation independent of its effect on cell survival.

Animals↗

Comparison of oligoclonal immunoglobulin G bands in multiple sclerosis cerebrospinal fluid by immunoblotting and immunofixation.

We previously analyzed unconcentrated cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and other neurologic diseases by isoelectric focusing in agarose gel. We have now developed an immunoblot method for detection of oligoclonal IgG bands in unconcentrated MS CSF. The oligoclonal IgG band patterns seen after immunoblotting were compared with those of conventional immunofixation. Although immunoblotting was found to be rapid the resolution and intensity of oligoclonal IgG bands were somewhat better after immunofixation. Since immunofixation is simpler than immunoblotting, we recommend that clinical laboratories use immunofixation after isoelectric focusing to detect oligoclonal IgG bands in CSF.

Humans↗

Changes in bioelectric potentials on bone associated with direct current stimulation of osteogenesis.

This study was designed to determine whether changes occur in the bioelectric potentials on bone during and after bone stimulation with a 20-microA direct current (DC) and whether the variations in bioelectric potentials are related to the variations in bone formation. The bioelectric potentials were recorded at different times on the rabbit distal tibial surface, during (current-on state) and after (current-off state) DC stimulation with a cathode implanted within the medullary canal. The new bone formed at the end of the experiment was quantitated and related to the bioelectric potentials recorded at current-on and current-off states, respectively. Direct current stimulation resulted in electronegative potential spike centered on the cathode tip while current was applied. After electrical stimulation was turned off, the residual potentials at the end of the experiment did not significantly differ from the initial values. Conversely, the time sequence of the changes was significantly different from the control to the experimental group. The variations in the induced potentials at current-on state were significantly related to the variations in bone formation. This study suggests the existence of a relationship among bioelectric potentials, DC stimulation, and osteogenesis.

Animals↗

Studies on the stability of vinblastine sulfate in aqueous solution.

A stability study on vinblastine sulfate (1) in aqueous solution at several different temperatures (in the absence of light) has been undertaken. High-performance liquid chromatography was used to obtain kinetic data for the loss of vinblastine and to monitor the order of appearance of degradation products, of which eight were observed. The two most prominent components of early heat degradation have been tentatively identified by mass spectrometry (DCl), as a C19'-oxidation product (19'-oxovinblastine, 4, MH+ 825) and an isomer of vinblastine (MH+ 811). Products appearing later in the course of degradation include a component of MH+ 809, possibly corresponding to either 19'-hydroxy-3',4'-dehydrovinblastine (5) or 3',4'-dehydrovinblastine-6'-N-oxide (6), and three other products of MH+ 636, MH+ 670, and MH+ 795. In addition, optical rotation studies have established that racemization (with a possible reduction in biological activity) does not occur prior to transformation into degradation products. Preliminary experiments on light-mediated degradation demonstrated a different sequence of transformation, with the MH+ 670 and MH+ 795 products appearing first. Contrary to reports and suggestions in the literature and based on an estimated activation energy of 27.1 kcal/mol, the results of this study indicate that vinblastine sulfate is relatively stable in aqueous solution at or below room temperature (estimated t90 values: 150 d at 25 degrees C and 10.7 years at 5 degrees C) and has a t90 value of 16.6 d at 37 degrees C. These results are important in view of current interest in the continuous iv infusion of vinblastine sulfate.(ABSTRACT TRUNCATED AT 250 WORDS)

Chromatography, High Pressure Liquid↗