Search PubMed⌕ Search

Biomedical subjects

J Bishop

Publications and source records attributed to J Bishop.

At least 145 records · Page 8Linked to original sources
PubMed1984

A 45-kb DNA domain with two divergently orientated genes is the unit of organisation of the murine major urinary protein genes.

The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be distinguished by nucleic acid hybridisation. By screening a Charon 4A library of mouse DNA with probes from the 5'-flanking region of a MUP gene, we have isolated clones that contain both a Group 1 and a Group 2 gene, orientated in a divergent fashion, with 15 kb of DNA between the 5' ends of the genes. We show that this pairwise arrangement is the predominant organisation of MUP genes in the BALB/c genome. We argue that the head-to-head gene pair is the unit both of DNA organisation and of evolution. Taking into account the genes themselves, the intervening 15 kb and the homologous 3'-flanking regions, this unit is approximately 45 kb long. We also show that some MUP genes may be linked in a tail-to-tail fashion with 26-28 kb between the 3' ends of two genes. This suggests that the minimum distance between successive 45-kb units is approximately 7 kb.

Animals↗
PubMed1981

Detection of platelet alloimmunity with a platelet-associated IgG assay.

A quantitative immunofluorescence PA-IgG assay was used to detect alloimmunity to platelets. The assay identified serum alloantibodies in 10 out of 14 multitransfused patients and for two of three infants with neonatal thrombocytopenia. The correct separation of all multitransfused patients into alloimmune and nonalloimmune groups by the PA-IgG assay was substantiated with chromium-51--labeled platelet survival studies. The allogeneic nature of the serum antibodies was demonstrated by progressive absorption of the antibody with increasing numbers of allogeneic platelets but not with autologous platelets. The sensitivity of the PA-IgG assay for detection of serum alloantibodies was superior to that of platelet aggregation, platelet serotonin release, and lymphocytotoxicity testing. In dilution experiments with alloimmune serum, elevated levels of serum PA-IgG could still be detected on donor platelets when platelet aggregation and serotonin release tests became negative. Platelet survival studies with selected platelets performed in the 10 alloimmunized, multitransfused patients confirmed the results of the PA-IgG assays, predicting alloimmunity to the donor platelets. In contrast, platelet aggregation, platelet serotonin release, and lymphocytotoxicity testing indicated alloimmunity for 50% or less of the patients. Reduced platelet survival times were also seen with HLA A- and HLA B-matched donor platelets when donor-recipient incompatibility was demonstrated by the PA-IgG assay. Thus the PA-IgG assay provides a sensitive method to detect serum platelet alloantibodies and may offer a technique in platelet crossmatching.

Absorption↗
PubMed1977

The effect of public policies on the demand for higher education.

A binomial logit model is fitted to the college attendance behavior of 27,046 male high-school juniors in 1960, divided into 20 subgroups defined by student ability and family income. Tuition, high admissions standards, travel costs, and room and board costs all have significant negative effects on attendance. The highest elasticities of demand are found to occur in the low-income strata and lower-middle ability quartile, suggesting that an efficient subsidy program should focus on these groups. Coefficients of forgone earnings and measures of the local payoff to college attendance are small but generally have the expected sign.

Behavior↗
PubMed1976

Timing of single daily meal influences relations among human circadian rhythms in urinary cyclic AMP and hemic glucagon, insulin and iron.

Relations among circadian rhythms in serum iron, glucagon and insulin and urinary cyclic AMP excretion differ drastically when diurnally active, nocturnally resting human adults consume all daily food for one week as breakfast only and for another week as dinner only - a finding of interest to diverse fields, e.g., for optimizing certain kinds of therapy or for a better utilization of calories.

Circadian Rhythm↗
PubMed1975

Enhancement of filterability of sickle cells by cyanate: an effect independent of oxygen saturation.

Filterability and morphology of cyanate-treated sickle cells were compared to those of untreated cells at equal oxygen saturations to determine whether carbamylation inhibited sickling by an effect other than by its alteration of the oxygen dissociation curve. Morphology of treated and untreated cells was not significantly different at all levels of oxygen saturation examined. Filterability, on the other hand, was improved significantly by carbamylation. This latter finding indicates that carbamylation enhances deformability of sickle cells by a mechanism(s) in addition to its effect on red cell oxygen saturation. This mechanism(s) may account for the clinical benefit of cyanate therapy with doses which do not significantly affect the oxygen dissociation curve.

Adenosine Triphosphate↗
PubMed1972

Regulations controlling the sale of mild analgesics in Canada.

Some mild analgesic preparations are sold only on prescription; others are required to carry specific label warnings. The conditions of sale of some are further restricted by limitations on their dose. The "prescription only" sale of both phenacetin and acetaminophen would leave no "over-the-counter" analgesics available for individuals sensitive to salicylates. Since analgesic nephropathy results from long-term abuse of these preparations, the need is to prevent abuse without inhibiting their proper use.

Acetaminophen↗
PubMed1971

Methadone.

Explore the source record for details and available documents.

Canada↗