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Biomedical subjects

J Bilbao

Publications and source records attributed to J Bilbao.

14 recordsLinked to original sources
PubMed1992

Seizures of sleep onset: clinical and therapeutical aspects.

We studied the clinical characteristics and therapeutic response of 10 patients who presented with seizures shortly after falling asleep as the only or main manifestation of their epilepsy. The clinical evaluation was designed to investigate the appearance of seizures during diurnal and nocturnal sleep. The pharmacological response to coffee or amphetamine was investigated after normalization of previous anticonvulsant treatment. Seven of 10 patients (70%) presented with seizures during the siesta, and in 3 of 10, seizures occurred if they fell asleep at any time of the day. Seizures were detected in later hours of the night in five patients as sleep reinitiation seizures or seizures without previous awakening. The response to anticonvulsants alone was disappointing. The addition of coffee or amphetamine suppressed seizures of sleep beginning at night or during the siesta. Neither of them had an influence on sleep diurnal seizures outside of the siesta or on seizures of nocturnal sleep reinitiation. Suppression of coffee or amphetamine was followed by reappearance of the seizures.

Adult↗
PubMed1992

Permanent stained preparations of synovial fluid for detection of calcium compounds using alizarin red S.

Permanent preparations of air dried synovial fluids were prepared by staining calcium compounds with alizarin red S stain; each slide was coverslipped with Permount. Variables studied were: (a) concentration of the solution of alizarin red S, (b) pH of staining solution, (c) time of incubation in staining solution and aqueous and ethanolic content of staining solution. The staining effect of each solution was tested on calcium pyrophosphate dihydrate, calcium oxalate, apatite and monosodium urate (MSU). Of all the solutions, best results were obtained with 0.25% alizarin red S in 50% ethanol at pH 7.0 for 30 min. With this solution, the calcium-containing compounds were well stained. MSU did not stain and still preserved negative birefringence on polarization. Fixation of smears with ethanol served a double purpose: It fixed the slides without dissolving or removing MSU or the calcium compounds, yet it did dissolve five corticosteroids commonly used for intra-articular injection which may interfere with interpretation of compensated polarized light microscopy of synovial fluids.

Anthraquinones↗
PubMed1991

Effects of stimulation and inhibition of acid secretion on gastric damage induced by platelet-activating factor.

Intravenous administration of platelet-activating factor (PAF) induces extensive damage in rat gastric mucosa. The aim of the present study was to examine whether the presence or absence of acid in the gastric lumen could modify the PAF-induced gastric damage. The effects of inhibition of basal and pentagastrin-stimulated acid secretion by ranitidine on the deep histological gastric damage induced by 30 min of infusion of PAF (100 ng/kg/min) were assessed by using a histological score. Inhibition of basal gastric acid secretion did not prevent the histological gastric damage induced by PAF. Stimulation of gastric acid secretion by pentagastrin significantly increased PAF-induced gastric damage, and this effect was reversed by a dose of ranitidine that returns acid secretion to baseline levels. This acid-related damage was confined to the deep mucosa, since scanning electron microscope analysis ruled out an additional surface damage in PAF-infused rats when gastric acid was stimulated. The data indicate that a certain amount of acid may worsen the deep gastric mucosal damage induced by PAF.

Animals↗
PubMed1991

Combined Fontana-Masson-mucin staining of Cryptococcus neoformans.

Cryptococci react positively with various histochemical stains, including the Fontana-Masson (FM), which stains the cell wall, and mucin stains, such as alcian blue and mucicarmine, which stain the capsule. Combinations of the FM stain with both the alcian blue and mucicarmine stains were performed on paraffin-embedded tissue specimens that were obtained from 15 patients who had culture-proved cryptococcosis. Combined FM-mucicarmine and FM-alcian blue stains were compared with other individual fungal stains. The FM stain, followed by either the mucicarmine or alcian blue stain, distinctively demonstrated both the cell wall and capsule of most organisms. More organisms were recognized in the combined stains than with either stain done individually. No interference between the stains was noted. Combining the FM stain with either of these two mucin stains appears to be helpful for identifying cryptococci.

Alcian Blue↗
PubMed1991

[Is the biopsy of the temporal artery necessary? A study of 204 biopsies].

Due to the controversy on the need of performing temporal artery biopsies for the diagnosis of giant cell arteritis (GCA), we have reviewed 204 biopsies of temporal arteries as well as the clinical histories of the 190 patients. Patients without local manifestations were excluded from this study. From the 173 remaining patients, 54 presented a diagnostic biopsy of GCA. The 119 patients with a negative biopsy were divided into different groups according to the final diagnosis. From our data we could not find a clinical pattern which could permit the prediction of biopsy positiveness. We could not find either any clinical entity in the nondiagnostic group which would permit to exclude a diagnosis before performing the biopsy. We consider that the biopsy of the temporal artery can not be avoided nowadays and that it represents the only decisive diagnostic method of GCA.

Aged↗
PubMed1991

[Therapeutic usefulness of antioxidant drugs in experimental intestinal reperfusion syndrome].

The intestinal reperfusion injury has been studied in Sprague-Dawley rats weighing 200 g. Intestinal ischemia has been induced by clamping the Superior Mesenteric Artery (SMA) for 120 minutes. In order to parallel clinical situations, free radical scavengers (allopurinol [ALLO]) and superoxide-dismutase [SOD] were inoculated at a low perfusion rate through the femoral vein during the last 20 minutes of the ischemic period. Both drugs have decreased the mortality rate (from 70% in control group, to 40%) and the mean percentage of damaged intestine (30.89% vs. 23.84% and 24.70%). Histologically, ALLO was less effective than SOD (12.54 control; 8.40 SOD; 11.54 ALLO). The modification of glucose, SGOT, SGPT and LDH found in all the ischemic animals shows the hepatocellular injury induced by intestinal reperfusion.

Allopurinol↗
PubMed1991

[Regenerative response of hepatocytes implanted in the spleen].

Regenerative activity of syngenic hepatocytes inoculated into the spleen has been assessed. Partial hepatectomy and cyclosporine were used as regenerative stimuli. Nuclear DNA has been quantified by means of a cytophotometric method, and the intensity of the response has been defined as the Mean Percentage of Regenerating Hepatocytes (MPRH). Both hepatectomy and cyclosporine (alone or associated) have significantly increased splenic MPRH.

Animals↗
PubMed1991

Large-cell anaplastic (Ki-1-positive) lymphoma complicated by disseminated intravascular coagulation.

Large-cell anaplastic lymphoma (Ki-1-positive lymphoma) was first described as a type of large-cell lymphoma that has morphologic, enzymatic, and immunologic similarities to malignant histiocytosis. To date, an association between Ki-1-positive lymphoma and disseminated intravascular coagulation has not been described, but cases of malignant histiocytosis with disseminated intravascular coagulation have been reported. We report a case of anaplastic, Ki-1-positive, large-cell lymphoma complicated by clinical and laboratory evidence of disseminated intravascular coagulation with histologic evidence of vascular invasion and fibrin thrombi.

Antigens, CD↗
PubMed1989

Multiple focal nodular hyperplasia of the liver associated with vascular malformations of various organs and neoplasia of the brain: a new syndrome.

Focal nodular hyperplasia (FNH) is a lesion of the liver in which a large anomalous artery is located within a region of hyperplastic hepatic parenchyma. Patients with FNH commonly have other lesions, often vascular in nature, in the liver or other organs. We have noted that these associated lesions almost always occur in patients with multiple FNH. We therefore studied 27 autopsied patients with FNH. All 13 with multiple FNH had other lesions such as hemangioma of liver, meningioma, astrocytoma, telangiectasis of the brain, berry aneurysm, dysplastic systemic arteries, and portal vein atresia. One patient had several of these lesions including multiple FNH, meningioma, astrocytoma, vascular malformation of the brain stem, and hemangioma of the liver. In contrast, among the 14 patients with solitary FNH there were no associated lesions, except for hepatic hemangioma in one patient. The prevalence of this syndrome was estimated by examination of 2500 serial autopsies and autopsies with various components of the syndrome. On review of 73 consecutive autopsies with meningioma, three had multiple FNH, compared with seven of 2500 consecutive adult autopsies (P less than 0.001). Multiple FNH was found in two of 83 autopsies with astrocytoma (P less than 0.05) and in one of 139 autopsies with berry aneurysm (not significant). We describe a telangiectatic subtype of FNH which occurs in this syndrome as well as in a minority of patients with solitary FNH. The existence and character of this syndrome suggest that there may be an underlying systemic abnormality in some patients having components of the syndrome. Investigation of patients with multiple FNH lesions may reveal significant treatable lesions.

Adolescent↗
PubMed1985

Reversible, late-onset disulfiram-induced neuropathy and encephalopathy.

Disulfiram toxicity is a well-recognized cause of peripheral neuropathy and encephalopathy, usually developing within a few months of the start of therapy. We describe a patient who insidiously developed a peripheral neuropathy and encephalopathy after thirty years of disulfiram ingestion. Both complications partially resolved after the medication was stopped.

Aged↗
PubMed1976

Click evoked eighth nerve and brain stem responses (electrocochleogram)--experimental observations in the cat.

The origin of waves two to five occurring in the first 10 milliseconds after repetitive click stimuli is not definitely established. Published studies have suggested that waves two and three are derived from the cochlear nucleus and superior olivary complex respectively and that waves four and five are generated by the inferior colliculus. Our studies of the effect of chronic brain stem lesions in the cat do not support this hypothesis of specific localization, but reveal that a more general reduction in amplitude, and at times prolongation in latency, occurs with lesions in the pons and lower midbrain and that wave one amplitude is also diminished with brain stem lesions.

Animals↗