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Biomedical subjects

J Beuth

Publications and source records attributed to J Beuth.

At least 55 records · Page 3Linked to original sources
PubMed1995

[In vitro activity of Mercurius cyanatus complex against relevant pathogenic bacterial isolates].

The antimicrobial activity of mercurius cyanatus complex (Oligoplex) and its components Mercurius cyanatus D5, Echinacea angustifolia D1, Ailanthus glandulosa D3, Ammonium bromatum D3, Baptisia tinctoria D3, Euspongia officinalis D2, alcohol 5% (dilution: D1 = 1: 10, D2 = 1 : 100 etc.) was tested in vitro by serial dilution tests against 105 clinical isolates (grampositive/negative, aerobes and anaerobes with relevance for pharyngitis). The bactericidal activity was compared with that of vancomycin when appropriate. One component of the composition (Mercurius cyanatus) exerted a considerable bactericidal activity against S. pyogenes, S. agalactiae, S. pneumoniae, S. aureus, E. faecalis in serial dilutions of the clinical relevant concentration D5. However, growth of H. influenzae, Bacteriodes sp. and Actinobacillus actinomycetemcomitans was not inhibited by Mercurius cyanatus and any other component of the composition. The composition, however, exerted a bactericidal range similar to that of Mercurius cyanatus, but less efficient. Analysis of the bactericidal effect of Mercurius cyanatus and vancomycin revealed comparability for S. pyogenes, S. agalactiae, S. pneumoniae, S. aureus and E. faecalis for vancomycin concentrations of 0.063-2 mg/l, which are clinically relevant.

Anti-Bacterial Agents↗
PubMed1995

[Immunoactive effects of various mistletoe lectin-1 dosages in mammary carcinoma patients].

Cellular aspects of the immunomodulating activity of a proprietary mistletoe extract (Eurixor) standardized for mistletoe lectin-1 (ML-1) were investigated in patients suffering from mammary carcinoma (n = 20). Regular subcutaneous injections of the different dosages (0.5 and 1.0 ng ML-1/kg body weight, twice a week, for 5 weeks) yielded statistically significant increases of defined peripheral blood lymphocyte subsets (helper T-cells, natural killer (NK)-cells) which are generally believed to be involved in antitumor activity. Moreover, administration of either ML-1 concentration resulted in enhanced expression of activation markers such as interleukin-2 receptors and HLA/DR-antigens on peripheral blood T-lymphocytes. This study suggests that regular subcutaneous administration of both ML-1 concentrations (0.5 and 1.0 ng/kg body weight) can efficiently stimulate the cellular immune system of cancer patients.

Adjuvants, Immunologic↗
PubMed1995

Immunomodulating ability of galactoside-specific lectin standardized and depleted mistletoe extract.

Commercially available mistletoe extract standardized for the galactoside-specific lectin (ML-1; Eurixor) and a chromatographically ML-1-depleted preparation (same charge no. and composition of remaining components) were tested for their immunomodulating potency. In BALB/c-mice, regular subcutaneous administration of the optimal immunomodulating dosage (1 ng ML-1/kg body weight) could be shown to induce no influence on spleen weight, a non-significant increase of thymus weight, a significant increase of thymocyte, peritoneal macrophage, peripheral blood leukocyte, lymphocyte and monocyte and monocyte counts, and a significant decrease of peripheral blood granulocyte counts. Administration of analogue volumes (concentrations) of ML-1-depleted extract, however, did not induce any immunopotentiation. Accordingly, it may be assumed, that the galactoside-specific lectin (ML-1) represents the main immunomodulating component in commercially available mistletoe extracts.

Adjuvants, Immunologic↗
PubMed1994

Combined immunomodulation (Propionibacterium avidum KP-40) and lectin blocking (D-galactose) prevents liver tumor colonization in BALB/c-mice.

The protective effect of combined treatment (immunomodulation with Propionibacterium avidum KP-40; liver lectin blocking by D-galactose administration) on the liver colonization of RAW 117-H10 lymphosarcoma was investigated in BALB/c-mice. Both, immunomodulation with P. avidum KP-40 as well as liver lectin blocking by D-galactose treatment significantly decreased the number of liver tumor colonies in this experimental model. However, the combination of P. avidum KP-40 and D-galactose obviously proved to be superior to each monotherapy since the liver colonization by RAW 117-H 10 lymphosarcoma could be completely inhibited.

Animals↗
PubMed1994

Cell electrophoretic discrimination of Staphylococcus saprophyticus strains of different origin.

Staphylococcus saprophyticus isolates (n = 10) from urinary tract infections (UTIs) could be reproducibly distinguished from S. saprophyticus strains from respiratory tract infections (n = 5) by distinct electrophoretic mobility. Thus, the method of "bacteriopheresis" may offer a new approach to discriminate pathogenic microbial strains of different origin with respect to defined surface structures (hemagglutinins, lectins), generating distinct surface charges.

Electrophoresis↗
PubMed1994

[Immunoactive action of mistletoe lectin-1 in relation to dose].

Galactoside-specific mistletoe lectin-1 (ML-1) was isolated by affinity chromatography from proprietary mistletoe extract and checked in BALB/c-mice for its immunoactive potency. To investigate the optimal immunomodulating dosage, ML-1 (0.5, 1.0, 2.5, 5.0 ng/kg body weight, b.w.) was subcutaneously administered for three subsequent days followed by another injection 48 h later. These studies proved that injections of 1 ng ML-1/kg b.w. induced optimal immunomodulation, since thymocyte proliferation, maturation and emigration were significantly enhanced in this murine model as compared to non-treated control mice. Further on, counts of peripheral blood lymphocytes and monocytes as well as expression of relevant activation markers on these cells revealed significant increases after ML-1 (1 ng/kg b.w.) administration. However, increase of cell counts and activity of peritoneal macrophages were less pronounced but still statistically significant for this ML-1 concentration. Determination of immune responses after low dose ML-1 treatment (0.5 ng/kg b.w.) presented relevant (partly statistically significant) increases, too. However, high dose ML-1 treatment (2.5, 5.0 ng/kg b.w.) did not enhance (but suppress) relevant immune functions. For future clinical/therapeutical treatment strategies, ML-1 dosages ranging from 0.5-1.0 ng/kg b.w. may be supposed to be optimal.

Adjuvants, Immunologic↗
PubMed1993

Hepatocellular injury inhibits lectin-mediated tumor colonization into BALB/c-mice livers.

Acute (hepatitis) and chronic (cirrhosis) liver injuries were experimentally induced in BALB/c-mice by administration of D-galactosamine and carbon tetrachloride, respectively. In both experimental liver diseases the incidence of hepatic tumor colonization of sarcoma L-1 was significantly reduced as compared to non-treated control animals. Thus, it seems that either dysfunction or loss of organ-characteristic lectins (galactosyl-specific hepatic lectins) prevented liver colonization. Histochemical staining of liver sections from D-galactosamine or carbon tetrachloride-treated mice with appropriate galactose-containing (neo)glycoproteins supported this hypothesis, since the lectin-dependent binding was greatly reduced as compared to sections from non-treated animals.

Animals↗
PubMed1993

[Effect of mistletoe lectin therapy on serum level of defined serum proteins (acute phase proteins) in tumor patients].

The influence of galactoside-specific mistletoe lectin (ML-1) administration on defined acute phase reactants in the serum of cancer patients (mammary carcinoma, n = 4; larynx carcinoma, n = 11; TNM-stages II to IV; after appropriate surgery, chemotherapy, radiation) was studied. Regular subcutaneous injections of the optimal doses of ML-1 (1 mg/kg body weight, twice a week) yielded statistically significant increases of certain acute phase reactants (C-reactive protein, haptoglobin, coeruloplasmin, C3-complement, albumin, immunoglobulin IgM) after four weeks of treatment. However, serum concentrations of transferrin, C4-complement and the immunoglobulins IgG and IgA were found within the biological range (means +/- 2 s). The increase of acute phase reactants after administration of ML-1 correlates positively with the activity of lymphatic cells (e.g. expression of IL-2 and HLA-DR receptors) in FACS (fluorescence-activated cell sorter) staining experiments and indicates the immunoreactive potency of this substance which may be speculated to be cytokine-induced.

Acute-Phase Proteins↗
PubMed1993

Effect of purinergic receptor antagonists suramin and theobromine on tumor-induced angiogenesis in BALB/c mice.

The purinergic receptor antagonists suramin (SRN) and theobromine (TBR) were examined for their anti-angiogenic activity in BALB/c mice. SRN or TBR were subcutaneously administered to BALB/c mice in doses of 1-125 mg/kg body weight on days 0, 1 and 2 after intradermal inoculation of E14/W lung carcinoma cells. It was shown that SRN and TBR inhibited tumor-related angiogenesis. Accordingly, it may be suggested that purinoceptor antagonists may inhibit neovascularization in tumor growth and metastasis.

Animals↗
PubMed1993

[Comparative studies on the immunoactive action of galactoside-specific mistletoe lectin. Pure substance compared to the standardized extract].

Comparative Studies on the Immunoactive Potency of Galactoside-specific Lectin from Mistletoe/Pure substance against standardized extract. Cellular and humoral aspects of the immunomodulating activity of the galactoside-specific lectin from mistletoe (ML-1) were investigated in cancer patients suffering from mammary carcinoma and compared to the immunoactive potency of a proprietary mistletoe extract standardized for ML-1 (ML-1 stand., Eurixor). Regular subcutaneous injections of the optimal dose of ML-1 and ML-1 stand. (1 ng/kg body weight; twice a week; for 4 weeks) yielded statistically significant increases of certain lymphocyte subsets (helper T-lymphocytes, natural killer (NK)-cells) which are generally believed to be involved in antitumor activity. Moreover, administration of either ML-1 preparation resulted in enhanced expression of interleukin (Il)-2 receptors on lymphatic cells and significantly increased serum levels of defined acute phase reactants (c-reactive protein, haptoglobin, C3 complement) as indicator of cellular and humoral activity. In vitro, exposition of human lymphocytes to ML-1 and ML-1 stand. resulted in enhanced expression of Il-2 receptors, which substantiated the capacity of both ML-1 preparations to affect immunological parameters within the host defense system. The effects of ML-1 and ML-1 stand. were comparable.

Acute-Phase Proteins↗
PubMed1993

[Immunomodulating effects of antibiotics influencing digestive flora].

Mucosal surfaces are habitats of the physiological microflora and are closely related to the mucosal immune compartment (mucosa-associated lymphoid tissue, MALT). Recently, considerable evidence has been accumulated showing that various members of the physiological microflora liberate low molecular weight peptides which, apparently, are essential for adequate immune responses of the host. Antibiotic decontamination (e.g. of the BALB/c-mouse intestinal tract) results in a lack of generation of immunopriming microbial peptides leading to immunosuppression. Biochemical analysis of the peptides revealed reproducible chromatographic fractions which selectively influence maturation, proliferation, and activation of lymphatic cells.

Adjuvants, Immunologic↗
PubMed1993

[The importance of lectins for formation of tumor metastases and bacterial infection processes].

Adhesion of bacteria and adhesion of tumor cells have much in common, especially the participation of lectins in this process. In the future it might be possible to inhibit the metastatic process into the liver (e.g. during surgical operations of malignant tumors) and bacterial adherence to mucosal linings or plastic devices by blocking of adhesion molecules (lectins) with appropriate glycoconjugates. Initial clinical trials are very promising.

Bacterial Adhesion↗
PubMed1992

[Urinary tract infections caused by Staphylococcus saprophyticus. Increased incidence depending on the blood group].

Between 1. 1. 1988 and 31. 12. 1990, greater than or equal to 10 colony-forming units of Staph. saprophyticus were isolated from 55 of a total of 20,000 urine samples went in. The isolates from these 55 patients (52 women, 3 men; mean age 29 [17-58] years) were tested for specific adhesion molecules (lectins) to discover their relationship to blood-group characteristic carbohydrates. 52 of the 55 patients (94.5%) were blood-group A or AB (average for Middle-Europeans: 48.2%). Haemagglutination and inhibition tests with the Staph. saprophyticus isolates demonstrated in all instances N-acetylgalactosamine (GalNAc) specificity of the surface lectins. These findings support the hypothesis that the carbohydrate pattern of Blood group A (terminal GalNAc) is important for the colonization of exposed organs by Staph. saprophyticus with GalNAc-specific lectins.

ABO Blood-Group System↗
PubMed1992

Behavior of lymphocyte subsets and expression of activation markers in response to immunotherapy with galactoside-specific lectin from mistletoe in breast cancer patients.

Cellular aspects of the immunomodulating activity of the galactoside-specific lectin from mistletoe (ML-1) were investigated in 10 cancer patients. Regular subcutaneous injections (4 weeks) of the optimal dosis of ML-1 (1 ng per kg body weight, twice a week) yielded notable increases in the apparent numbers of certain lymphocyte subsets [pan T cells; helper T cells; natural killer (NK) cells] which are generally believed to be involved in antitumor immunity. Moreover, ML-1 administration resulted in an increased level of expression of interleukin (IL)2 receptors on lymphatic cells, an indicator of cellular activation. In vitro, the exposure of human lymphocytes to ML-1 resulted in an enhanced expression of receptors for IL-2 (T cells) and HLA-DQ (B cells), which similarly substantiated the capacity of ML-1 to affect immunological parameters within the host defense system. Thorough clinical trials are now required to assess any impact of the application of the lectin on the course of the disease.

Biomarkers↗
PubMed1992

Adhesion of fimbriated and non-fimbriated Klebsiella strains to synthetic polymers.

Adherence of three fimbriated and non-fimbriated Klebsiella strains to polyetherurethane was investigated in order to assess the possible role of fimbriae in the adhesion of Klebsiella to synthetic polymers. Fimbriated strains with type 1 and type 1.3 fimbriae adhere significantly stronger to polyurethane than the same strains lacking fimbriae, whereas a strain with type 3 fimbriae shows no different adherence compared with the non-fimbriated variant. Analysis of adhesion kinetics and isotherms reveals that adherence of fimbriated Klebsiella strains is proceeded by the formation of multicellular bacterial layers on the polymer surface. Measurements of the relative hydrophobicity of the strains and adherence experiments under exclusion of unspecific interactions point out that fimbriae obviously play a more important role in adhesion than relative hydrophobicity. The demonstration of reduction of bacterial adherence to polyetherurethane by blocking fimbrial action with fimbriae-specific sugars supports this further.

Bacterial Adhesion↗