Search PubMed⌕ Search

Biomedical subjects

J Best

Publications and source records attributed to J Best.

At least 91 records · Page 5Linked to original sources
PubMed1985

Factors influencing the sensitivity of herpes simplex virus detection in clinical specimens in a simultaneous enzyme-linked immunosorbent assay using monoclonal antibodies.

A rapid simultaneous enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies was investigated for herpes simplex virus (HSV) detection. All HSV isolated (n = 127) were detected, whereas no response was obtained with HSV negative preparations. Equivalent results were obtained from 275 of 277 clinical specimens in the monoclonal ELISA and in an ELISA using polyclonal antibodies, confirming that appropriately selected monoclonal antibodies may be as efficacious as polyclonal antibodies in antibody-based assays. In clinical specimens, the rate of HSV detection (sensitivity) relative to tissue culture isolation was low for both assays, and the major factor responsible for this was the low concentration of virus present in some specimens. The sensitivity of ELISA obtained in routine use varied with different panels of unselected specimens and was related to the speed of development of the cytopathic effect. These results emphasise the need for caution in assigning a definitive sensitivity level to ELISA tests evaluated on different panels of specimens.

Antibodies↗
PubMed1984

Diagnosis of pulmonary emphysema by computerised tomography.

Eleven patients with peripheral bronchial carcinoma had computerised tomographic (CT) scans before operation. The resected specimens from six of these patients showed mild centri-acinar emphysema. Preoperatively the two groups had not differed significantly in spirometric findings or lung volumes. The frequency distribution curves of EMI numbers within the lung fields of patients who proved to have centri-acinar emphysema differed significantly from those of the group who did not, there being more pixels in the EMI range -450 to -500 in the emphysematous group (p less than 0.001). Detailed assessment of lung density by CT scanning may be useful in the diagnosis of emphysema in life.

Adult↗
PubMed1982

Further studies of the airborne fungi and pollens of the Washington, D.C. metropolitan area.

Airborne fungi and pollen grains were studied during 1981 in two locations in the Washington, D.C. metropolitan area. This is a follow up of a 1977 survey of the same area to determine the prevalence of types of fungi and pollens. Dominant fungi were Hormodendrum (October), Penicillium (July) and Alternaria (September), whereas predominant pollens were oak (April), pine (April) and ragweed (September).

Air Microbiology↗