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J Bertoglio

Publications and source records attributed to J Bertoglio.

83 records · Page 5Linked to original sources
PubMed1976

Prognostic value of mouse red cell rosette formation in chronic lymphocytic leukaemia.

Mouse red cell (MRC) rosette formation was studied in 20 malignant haemopathies. Only cells from chronic lymphocytic leukaemia (CLL) and hairy cell leukaemia (HCL) formed MRC rosettes with a high frequency. However, cells from 2 cases of CLL could not be demonstrated to form MRC rosettes, although they were undoubtedly found to be of B origin. These 2 cases presented as typical CLL, but they were observed to transform into a sarcomatous type after 5-6 months of evolution, suggesting that the study of MRC rosette formation might be of prognostic value in CLL.

Adolescent↗
PubMedDate not supplied

Secretory IgA deficiency in pediatric patients: clinical and laboratory follow-up.

This is a prospective three-year (1985-1988) clinical and laboratory follow-up study of 43 pediatric patients without any measurable secretory IgA, intending to describe the their natural course. We also intended to detect evidence which may allow to predict their future outcome, whether they would become asymptomatic or end up developing chronic disease. A direct statistical correlation was found between those patients who normalize their secretory IgA levels and their course in an asymptomatic state. Thus, if a child does not have any sIgA at all, the relative risk to get sick is 0.86 (86%), while in those having sIgA within normal ranges, the relative risk of disease decreases to 0.46 (46%), representing a p value less than or equal to 0.05. Among the analyzed variables and their influence on the fact that a patient may or may not synthesize sIgA, none of them showed a predictive value for bronchial asthma by itself. However, when sIgA and total serum IgE levels, were analyzed together (being the two variables demonstrating to influence), the probability of becoming asthmatic is much greater in the ones with elevated total serum IgE and absent sIgA. This group is significantly different from the one with normal IgE and sIgA levels (p less than or equal to 0.001). According to our experience in evaluating and controlling a pediatric patient with repeated episodes of bronchial obstruction and lacking sIgA, an immediate strict environmental control should be established in order to avoid all possible contacts with allergens.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗
PubMedDate not supplied

[Cytotoxic antibodies to leukemia cells in normal human sera].

Antibodies cytotoxic for human leukemia cells were demonstrated in 7 parents of leukemia children and in 2 normal donors whose blood had induced a partial remission in leukemia and lymphoma patients. None of these sera were cytotoxic to remission cells nor to normal lymphocytes. These sera appear to be relatively specific for acute lymphoblastic leukemia cells; however, several antigenic specificities appear to be detected by these sera.

Antibodies, Neoplasm↗
PubMedDate not supplied

Evaluation of secretory IgA childhood respiratory diseases.

Secretory IgA salivary concentration was studied, in order to investigate its influence as a possible causative role in groups of children affected by different respiratory diseases. Allergic bronchial asthma (n = 37), recurrent pneumonias (n = 11), recurrent upper airway infectious (n = 12) and a control group of healthy children (n = 25) were included. The sIgA mean concentration plus S.D. were: 26.0 +/- 11.5, 15.5 +/- 16.7, 21.2 +/- 15.6, 16.2 +/- 12.7 and 30.8 +/- 10.1, respectively. A statistically significant association between decreased or absent levels of sIgA and disease was found, but no correlation between alterations in sIgA concentrations and any class of serum immunoglobulin levels, or with any other analyzed variables could be established. Among the group of diseases studied, a statistically significant difference (p less than 0.01) was found between infectious asthma and controls, recurrent upper airway infections and controls, and between allergic and infectious asthma. These findings support the conclusion that a decrease or absence of sIgA is an important factor to be considered in infectious asthma, as well as in recurrent upper airway infectious diseases affecting children.

Asthma↗
PubMedDate not supplied

[Antigens associated with human leukemia].

Several clinical observations, such as spontaneous remissions, or transfusion induced remissions and the results obtained by active immunotherapy, suggest that human leukaemia cells may possess tumour specific antigens. In vitro studies have provided evidence for immune reactions to leukaemia in man. However, the antigens involved in such reactions are far from being fully identified. Serological studies of the human leukaemia associated antigens have been developed with xenoantisera raised in a variety of animal species, and with human sera obtained either from patients or from some normal individuals. It appears that the xenoantisera and the few human sera yet obtained do not detect the same antigenic structures. Schematically, one can assume that the rabbit antisera react with antigen(s) common to acute leukaemias or even to the four main leukaemia types; primate antisera react with the lymphoid leukaemias (both acute and chronic) or with the myeloid leukaemias. On the other hand, human sera appear to react in a different way, and several antigenic specificities may thus be detected on the cells of acute lymphoblastic leukaemias. However, these human sera are yet rather rare and do not cover all the varieties of leukaemias. Cross-reactivity with antigens of animal oncornaviruses or of animal leukaemias has been shown with some of the antisera used. The serological identification of human leukaemia associated antigens aims at two different goals: a better control of the promizing immunotherapy attempts as well as a new insight in the etiology of human leukaemias.

Animals↗
PubMedDate not supplied

Biological and receptor-binding activities of human interleukin-2 mutated at residues 20Asp, 125Cys or 127Ser.

We have used site-directed mutagenesis to analyse structure-function relationships of the human Interleukin-2 molecule. The mutations introduced targetted residue 20Asp, within the N-terminal A helix, as well as residues 125Cys and 127Ser in the C-terminal D helix. The results presented here demonstrate that destabilizing the C-terminus alpha helix through introduction of Pro residues in either positions 125 or 127 reduced the biological activity of IL-2 by a factor of 10 that was correlated with a decreased ability to bind the receptor. A number of substitutions in position 20 have an even more drastic effect on biological activity and receptor binding. However, specific substitutions such as 20Asn and 20Leu displayed a differential effect on human or mouse IL-2 receptors. Furthermore, 20Leu IL-2 was found to behave as a partial antagonist of natural IL-2 when tested on murine cells.

Animals↗