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J Bernard

Publications and source records attributed to J Bernard.

At least 721 records · Page 40Linked to original sources
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Biology of isolated immunocytes. II. Simultaneous detection of cell surface Ig and theta-antigen by immunoperoxidase staining at the ultrastructural level.

Surface Ig and theta-antigen of lymphocytes collected on the bottom of Beem capsules were stained by an indirect immunoperoxidase technique to compare B and T cells at the ultrastructural level. To distinguish the two markers, the surface Ig was allowed to cap during the incubation with anti-Ig antiserum while the incubation with anti-theta alloantiserum was performed at 4 degrees to obtain a staining in a ring pattern. This method was applied to the study of lymphocytes from spleen, lymph nodes and thymus of normal mice. Lymphocytes forming rosettes with SRBC were also studied at different days after immunization with SRBC.

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Biology of isolate immunocytes. III. Fine immunocytological characterization of lymphoplasma cells.

A system for characterization of lymphoplasma cells based on ultrastructural, immunocytological and autoradiographic data is proposed. It is composed of six cell types and transitional forms. Any mouse lymphocyte can be classified within one of these forms and the lymphocytes of a lymphoid organ may be subdivided into populations of characteristic cells. The cell types are defined as follows: 1) the differentiated Ig lymphocytes which bear Ig at their surface, have a rough nuclear sap, a granular cytoplasm and often show mcrovilli at the cell surface; none of these lymphocytes incorporate thymidine and therefore this subpopulation can be considered as mature; 2) the differentiated plasmacytes have a well organized rough endoplasmic reticulum; differentiated plasmacytes are not 3H-thymidine labelled and this subpopulation is mature; 3) the differentiated T lymphocytes with theta-antigen at their cell surface contain a smooth endoplasmic reticulum, a developed Golgi apparatus and lysophagosomes; the differentiated T lymphocytes show no 3H-thymidine labelling and this subpopulation is also mature; 4) in the large lymphocytes without surface Ig nor theta-antigen, chromatin aggregate are intermingled to the nuclear sap and a large nucleolus is present; this subpopulation of lymphocytes, a number of which show usually 3H-thymidine labelling, is immature; 5) the small lymphocytes without surface Ig nor theta-antigen have a large nuclear cytoplasm ratio; like the large lymphocytes, this population is also immature, since a percentage of these cells usually incorporates thymidine; 6) small T lymphocytes are labelled by the theta-antigen marker; after antigenic stimulation some of them are found to incorporate thymidine. Transitional forms between two cell types are also observed. They are more numerous immunization. This system of cell characterization is an attempt to correlate structure with function of lymphocytes withing the T and B cell concept of experimental immunology.

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[Morphometric analysis of periosteocytic osteolysis: its application to the diagnosis of hyperparathyroidism].

The authors indicate the required methodologic conditions for the morphometric analysis of periosteocytic osteolysis, a reliable and specific feature of overactivity of the parathyroid gland. Indeed, significant periosteocytic enlargement has been found in 101 113 cases of primary hyperparathyroidism compared to 92 reference cases. The measurement of the size of the periosteocytic lacunae may be carried out either on hand of a micrometric eyepiece or of a picture analyser. It has to be carried out on decalcified bone sections of 5 microns thickness and it is necessary to measure at least 50 lacunae. The decalcification process unmasks an already partially decalcified crown of periosteocytic bone tissue the thickness of which is more important in the hyperparathyroidal bone than in normal bone. This zone has a peculiar collagen texture. The measurement of the peri-osteocytic enlargement is easy on stained sections of decalcified bone or on microradiographs of non-decalcified bone and, contrariwise, very dubious on stained sections of non-decalcified bone.

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