[Angiographic pictures of vessels [vascular] traumata (author's transl)].
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Biomedical subjects
Publications and source records attributed to J Bartos.
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By means of two biological diffusion methods an oxytetracycline (OTC) content was determined in the tissues of nine calves treated with a single i. m. OTC dose in the quantity of 15 kg-1 body mass. The preparation used for the treatment was Oxymykoin Spofa inj. ad us. vet. For the testing 2 collection strains -- B. cereus var. mycoides (ATCC 11778) and B. subtilis (ATCC 6633) were employed. The method using spore suspensions of the above strains was more sensitive. OTC was quantitatively determined in the samples by reading the standard curves. By evaluation of the two methods the following results were obtained: 5 to 10 days post injection all calves were positive. 15 days after the administration one out of 3 calves was positive. 20 days following the injection both calves were negative. The most frequent residue occurrence was recorded at the injection site, then in the kidney and liver. Spleen lung and bone marrow were completely without any finding. Maximum concentrations were found in the samples from the injection sites and kidney. In the other organs and muscles (noninjection sites) many times lower concentrations were found. Culinary and technological treatment of positive meat and liver samples revealed that already 15 minutes' boiling destroyed the microbiologically active OTC residues; on the contrary, after roasting OTC inactivation in the samples was not that univocal. Freezing and salting are utterly insufficient for OTC inactivation. A preslaughter withdrawal time of 20 days is recommended for the preparation tested.
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Using a modified Gartside diffusion method (1960) the incidence of the residues of antimicrobial substances was followed in two categories of calves commercially slaughtered at the Brno Slaughter House. For testing the following three collection strains were used: B. subtilis (ATCC 6633), B cereus, var. mycoides (ATCC 11778), Sarcina lutea (ATCC 9341). In light weight calves samples of bile, liver, kidney, diaphragmatic and hind limb muscles, in heavy calves, due to technical reasons, only bile, liver and diaphragmatic muscle samples were examined. Residues were demonstrated in 26.2% of 103 light (suckling) calves; the most frequent finding was in the bile (22.2%) and kidney (15.5%). Identical residue findings in the diaphragmatic muscles and in the skeletal muscles of the hind extremities in light calves correspond to the formerly described results in calves from emergency slaughter (Malíková et al. 1974). In heavy calves the residues were demonstrated in 41.7% of 156 examined; the most frequent finding was in the liver (42.3%) and in the diphragmatic muscles (34%). Under the present operation conditions of calf fattening using milk replacer Biosan 20 not even an interval of 17-20 hours between the last feeding and the slaughter proved sufficient for elimination of the antimicrobial substances from the bodies of the slaughter animals.
In the treatment of diarrhoea in new-born calves in 14 herds, the clinical effectiveness of the combination of streptomycin (1,000,000 i. u.) with furazolidone (0.2 g) was compared with that of streptomycin (1,000,000 i. u.) alone and furazolidone (0.2 g) alone in the peroral application to 710 calves twice daily. The use of the combination resulted in the recovery of 97.1% of 244 calves, the use of streptomycin alone healed 99.2% of 236 calves, and the application of furazolidone healed 97.4% of 230 calves. However, these results are not statistically significant. Taking into account the costs of treatment as well as the losses due to mortality, the treatment with streptomycin alone was more economical than the use of the comdone. The furazolidone therapy was more advantageous than the use of the combination only when evaluated from the viewpoint of the purchase price of calves.
In 11 herds, to 209 calves a preventive preparation, Skiabaryum-Spofa, was applied in a dose of medium heaped soup spoon once a day for six days. Compared with the control group of 202 calves, morbidity in the experimental group was lower by 20.7 p. c. The difference was statistically conclusive (P = 0.01). In the case of therapeutic application, the same preparation in a dose of two medium heaped soup spoons twice a day cured 95.2 p. c. of the 209 calves on the average within 3.2 days. Mortality of the number of examined calves amounted to 1.5 p.c. Prevention by means of the Skiabaryum-Spofa preparation was more economic compared with the control group without any prevention.
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