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Biomedical subjects

J Bao

Publications and source records attributed to J Bao.

115 records · Page 7Linked to original sources
PubMed1992

Isolation and characterization of endogenous modulators for GABA system.

Pig brain extracts from both soluble and membrane fractions were found to contain potent inhibitors for GABA synthesizing enzyme, GAD, referred to as endogenous GAD inhibitors (EGIs) and for the binding of GABA agonist, muscimol, referred to as muscimol binding inhibitors (MBIs). EGIs and MBIs were first purified through gel-filtration Bio-Gel P-2 columns, in which multiple activity peaks were observed. One of them appears to be co-eluted with either L-glutamate or GABA. However, others are clearly separated from L-glutamate or GABA. EGIs were found to be low MW (less than 1,800 dalton), heat and acid-base stable, negatively charged, non hydrophobic substances. MBIs were found to be low MW (less than 1,800 dalton) neutral or positively charged substances. MBIs had no effect on [3H]flunitrazepam (FNZP) binding, indicating that they are not endogenous benzodiazepine receptor ligands and they may act specifically on GABA binding site.

Animals↗
PubMed1992

[Effects of serotonin and norepinephrine on neuronal discharges of the nucleus tractus solitarii in medullary slices].

Effects of serotonin (5-HT) and norepinephrine (NE) on neuronal activities of the nucleus tractus solitarii (NTS) were investigated in rat medullary slice preparations. The spontaneous discharges after perfusion of slices with 5-HT (1 mumol.L-1, 3 min) were increased in 25 (58.1%), reduced in 13 (30.2%), and unchanged in 5 (11.7%) neurons. Afer perfusion of slices with NE (1 mumol.L-1, 3 min), the spontaneous discharges were reduced or ceased in 27 (62.8%), increased in 13 (30.2%), and unchanged in 3 (7.0%) neurons. Responding to both 5-HT and NE were 38 (88.4%) neurons, among which 21 were selected for studying the effect of ketanserin (a new 5-HT2 blocker). The spontaneous discharges were more increased in 10 (47.6%) and reduced in 4 (19.0%) neurons after perfusion of ketanserin+NE than after perfusion of NE alone. These results indicate that there is an interaction between 5-HT2 receptor and alpha 2 adrenoceptor at NTS level, suggesting that the central action of ketanserin may be one of its antihypertensive mechanisms.

Animals↗
PubMed1991

Structure and function of L-glutamate decarboxylase.

Membrane bound L-glutamate decarboxylase (GAD) has been solubilized and partially purified from hog brain. The solubilized GAD appears to exist in two forms, alpha and beta, differing in their size and electrophoretic mobility. The alpha form has similar mobility as that of the soluble GAD in 7.5% and 5-25% gradient polyacrylamide gel electrophoresis suggesting that they are similar in size and charge. In addition, gene encoding for mouse brain GAD has been cloned and characterized. Mouse brain GAD cDNA consists of two DNA fragments with 1.6 and 1.0 Kb. The 1.6 and 1.0 Kb fragments contain 1657 and 974 bP, respectively. The significance of multiple forms of GAD is also discussed.

Animals↗
PubMed1991

[A simple and sensitive DNA-RNA hybridization method--in situ hybridization on the nitrocellulose filter for detecting oncogene expression in tumor cells by using digoxigenin-labelled probes].

A simple and sensitive method for detecting oncogene mRNA is performed by fixing tumor cells directly onto nitrocellulose paper, followed by in situ RNA hybridization with digoxigenin-labelled oncogene probes. The human lung large cell carcinoma cell line PLA801-D95 (before and after induction of differentiation with retinoid acid) and leukemic cells from clinical patients were used as target cells. The results showed that 5000 cells were sufficient to give a positive signal for oncogene mRNA. Compared with RNA dot blot, it was found that this technique was quick, simple, sensitive and reproducible. It can be used to study gene expression in tumor cells as well as other cells.

Carcinoma↗
PubMed1991

[Long-term changes in the different types of coronary diseases treated with traditional Chinese and Western medicine].

64 cases with TCM differential types of CHD treated with TCM-WM in long term (an average of 10.5 years) were divided into four types according to deficiency of Yang [including weakness of Q1], deficiency of Yin, blood stasis and disorder of Qi and phlegm. Compared with pre-treatment 42 cases (65.62%) with TCM differential types showed improvement or no change in group I, II (clinical total effective rate 92.86%, the significantly effective rate 40.98%). While 22 cases (34.38%) with TCM differential type became aggravated or worse (clinical total effective rate 54.54%, the significantly effective rate 13.63%). The report indicated that the results in comparing group I, II with group III, IV were statistically significant; age (P less than 0.01), course of disease (P less than 0.01), impairment of myocardium (P less than 0.05), complication (P less than 0.05), ratio of deficiency of Yang (P less than 0.01), blood stasis (P less than 0.01) and high coagulation of blood (P less than 0.01). Therefore the author suggests that it is necessary to protect Yang-Qi and prevent and treat the complication of the CHD patients.

Aged↗
PubMed1990

[15 years' observation and related research on diagnosis and treatment according to traditional Chinese medicine of elevated serum lipids in 54 cases of coronary disease].

The increasing rate of cholesterol (CH) of 54 cases with coronary heart disease was 33.33%. According to CH level, the authors divided them into two groups: the normal one (I) and the high one (II). By fifteen-year observation, CH of Group I kept normal, only with its triglyceride (TG) a little higher than ordinary level. But the CH and TG of Group II, which had been treated with anticholesteremic drugs, was obviously higher than Group I (P less than 0.05). In connection with identification according to TCM, both CH and TG of deficiency of Yang, and deficiency of both Yin and Yang were higher, especially TG (P less than 0.01). TG of the type of blood stasis was higher (P less than 0.01) except anticholesteremic drugs for Group II. The two groups were treated with the same combined treatment of TCM and WM. The long term effect of fifteen years in Group I was more obvious than in Group II. This showed that the treatment of high serum lipid of CHD was more difficult.

Adult↗