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Biomedical subjects

J Bao

Publications and source records attributed to J Bao.

At least 91 records · Page 5Linked to original sources
PubMed1995

Brain L-glutamate decarboxylase. Inhibition by phosphorylation and activation by dephosphorylation.

Previously we showed that the activity of the gamma-aminobutyric acid-synthesizing enzyme L-glutamate decarboxylase (GAD) in crude brain extract is inhibited by ATP and protein phosphatase inhibitors. We suggested that GAD activity is regulated by protein phosphorylation. In this paper we further present evidence to support our hypothesis that protein kinase A and calcineurin may be involved in regulation of GAD activity through phosphorylation and dephosphorylation fo GAD, respectively. In addition, the effect of neuronal stimulation on GAD activity in cultured neurons is also included. A model to link neuronal excitation and activation of GAD by Ca(2+)-dependent phosphatase is proposed.

Animals↗
PubMed1995

Widespread expression of Huntington's disease gene (IT15) protein product.

Huntington's Disease (HD) is caused by expansion of a CAG repeat within a putative open reading frame of a recently identified gene, IT15. We have examined the expression of the gene's protein product using antibodies developed against the N-terminus and an internal epitope. Both antisera recognize a 350 kDa protein, the predicted size, indicating that the CAG repeat is translated into polyglutamine. The HD protein product is widely expressed, most highly in neurons in the brain. There is no enrichment in the striatum, the site of greatest pathology in HD. Within neurons, the protein is diminished in nuclei and mitochondria and is present in the soluble cytoplasmic compartment, as well as loosely associated with membranes or cytoskeleton, in cell bodies, dendrites, and axons. It is concentrated in nerve terminals, including terminals within the caudate and putamen. Thus, the normal HD gene product may be involved in common intracellular functions, and possibly in regulation of nerve terminal function. The product of the expanded allele is expressed, consistent with a gain of function mechanism for HD at the protein level.

Amino Acid Sequence↗
PubMed1995

[Vitrectomy combined with scleral buckling for the treatment of complicated retinal detachment].

PURPOSE: To raise operative successful rate of complicated retinal detachment, we used the methods of vitrectomy combined with scleral buckling for the treatment of complicated retinal detachment. METHODS: The operative methods included: scleral buckling, vitrectomy, membrane peeling, intraocular and extraocular drainage, gas and silicone oil tamponade. RESULT: Being followed up for 1-16 months, 22 eyes got anatomic reattachment in 29 eyes, the operative successful rate was 75.8%; 15 eyes were injected with silicone oil intraocular tamponade, with 12 eyes getting reattachment. The successful rate was 80%. CONCLUSION: Since the vitreous surgery is used, it is possible for the treatment of complicated retinal detachment, the operative successful rate can be raised. The technique of membrane peeling and intraocular tamponade was very important. Silicone oil has been accepted by more and more ophthalologists in the world, which acts as an effective material of intraocular tamponade.

Adolescent↗