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Biomedical subjects

J Baguena

Publications and source records attributed to J Baguena.

At least 19 recordsLinked to original sources
PubMed1982

[Liver explant cultures from rats of various ages (author's transl)].

Liver tissue from rats of different age has been cultured. The samples were obtained by surgical biopsy. A relationship between the obtained growth in culture and the animal age was established. A relationship between the obtained growth in culture and the animal age was established. The liver from young and weanling rats grows larger and earlier than the adult liver. The cells obtained in culture have been classified in five different types according to morphological criteria.

Age Factors↗
PubMed1981

Nonhistone protein changes during the diethylnitrosamine-induced carcinogenesis of rat liver.

Diethylnitrosamine (DENA) was administered with drinking water (40 mg/liter) to male Wistar rats for 4, 6, 8, and 10 weeks. The protein:DNA ratio and the ultraviolet light spectral properties of liver chromatin were not modified by DENA treatment. Nonhistone proteins were separated by sodium dodecyl sulfate:polyacrylamide electrophoresis on slab gels and analyzed by densitometry with a scanning microphotometer connected on line to a computer. There were no qualitative changes in the pattern of nonhistone proteins during the treatment with DENA. The quantitative changes statistically significant at p less than 0.005 were detected only in the 4th and 10th week, increases in fractions with molecular weights of 41,000 to 47,000 and 51,000 to 64,000 and decreases in fractions with molecular weights of 27,000 to 32,000 and 47,000 to 51,000 having been found. The proteinase activity of liver chromatin was assayed in incubation mixtures with 0.2 and 2 M NaCl and the measurement of the cleavage products was performed with ninhydrin. Proteolytic activity was found only in 0.2 M NaCl and was higher in rats treated for 8 weeks with DENA than in controls. Autolysis of chromatin for 24 hr at 37 degrees showed a severe breakdown of the nonhistone protein, being greater in the high-molecular-weight fractions than in the low-molecular-weight fractions.

Animals↗
PubMed1977

Fosfomycin in treatment of respiratory bacterial infections.

A study was made of 40 patients suffering from clinically and bacteriologically demonstrated bacterial respiratory infections which were all treated with fosfomycin as the only antibiotic. Studies were made of the sensitivity of the isolated organisms and control of the eradicated and selected organisms. Analytical controls were performed on all patients before, during and after treatment. The average dose employed was 60 mg/kg/day, divided into four doses and administered parenterally. The duration of the treatment fluctuated between 6 and 11 days in 87% of the cases. A clinical and bacteriological evaluation was made of the results. The clinical response was satisfactory in 75% of the cases, partial in 7.5% and poor in 17.5%. The bacteriological evolution was satisfactory in 62.5%, partial in 12.5% and poor in 20%. It was not possible to control the evolution in 15% of the patients. The concentrations of the antibiotic were determined in the sputum of 6 patients and fluctuated between 0.6 and 12.9 mug/ml. In no case could any significant disorders be attributed to the antibiotic.

Adolescent↗