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Biomedical subjects

J Baddiley

Publications and source records attributed to J Baddiley.

At least 109 records · Page 6Linked to original sources
PubMed1968

The glycerol teichoic acid from walls of Staphylococcus epidermidis I2.

1. Walls of Staphylococcus epidermidis I2 contain 30% (w/w) of a glycerol teichoic acid containing phosphate, d-alanine and d-glucose in the molecular proportions 1:0.25:0.50. 2. The teichoic acid was isolated by extraction with trichloroacetic acid and with dilute aqueous NN-dimethylhydrazine at pH7, and was shown to be a (1-->3)-linked poly(glycerol phosphate) containing beta-d-glucopyranosyl and d-alanyl ester substituents. 3. 2-O-beta-d-Glucopyranosylglycerol was isolated and characterized as its crystalline hexa-O-acetate. 4. Unlike that of certain other bacteria, the peptidoglycan component of the wall is not solubilized by NN-dimethylhydrazine. 5. The membrane teichoic acid is also a (1-->3)-linked poly(glycerol phosphate) but contains a smaller proportion of glucosyl substituents.

Alanine↗
PubMed1967

The phospholipids of Pneumococcus I-192R, A.T.C.C. 12213. Some structural rearrangements occurring under mild conditions.

1. The phospholipids from the non-capsulated strain of Pneumococcus I-192R, A.T.C.C. 12213, were separated into three fractions by chromatography on columns of silicic acid and DEAE-cellulose (acetate form). 2. The water-soluble phosphate esters produced by deacylation of each fraction were separated by chromatography on columns of DEAE-cellulose (HCO(3) (-) form). 3. Three deacylated products, diglycerol phosphate, glycerylphosphorylglycerol phosphate and bis(glycerylphosphoryl)glycerol, were identified by analysis, by chemical degradations and by comparison with synthetic materials. 4. From a study of freshly isolated lipids prepared and worked up under conditions where exposure to acid was minimal, it was concluded that the Pneumococcus contains phosphatidylglycerol and bisphosphatidylglycerol, in the molar proportion 1:2.5-3.0, and that the deacylation product glycerylphosphorylglycerol phosphate was probably an artifact of the isolation procedure. 5. Acid-catalysed isomerization (phosphodiester migration) of diglycerol phosphate and bis(glycerylphosphoryl)glycerol and transesterification (glycerol phosphate transfer) of diglycerol phosphate were observed. The structures of the products were established by degradation. 6. A novel mechanism for the biosynthesis of bisphosphatidylglycerol is presented.

Cellulose↗
PubMed1967

The structure and possible function of the glycolipid from Staphylococcus lactis I3.

1. The total lipid was extracted from Staphylococcus lactis I3 with chloroform-methanol mixtures and the glycolipid component was isolated by chromatography on silicic acid. 2. Saponification yielded a non-crystalline glycoside for which the structure O-beta-d-glucopyranosyl-(1-->6)-O-beta-d-glucopyranosyl-(1-->1)-d-glycerol has been established by chemical degradations and by comparison with synthetic material. 3. The role of the glycosyl diglycerides in bacterial membranes is discussed.

Chromatography↗
PubMed1966

3-O-methyl-D-mannose from Streptomyces griseus.

1. Streptomyces griseus was grown in a medium containing l-[Me-(14)C]methionine, and the labelled products from an ethanolic extract of the cells were examined. 2. Acid hydrolysis of one of the products gave a compound identified as 3-O-[Me-(14)C]-methylmannose by a series of degradative reactions. 3. Reduction of the radioactive compound gave 3-O-methyl-d-mannitol, indistinguishable from a synthetic sample.

Chemical Phenomena↗
PubMed1966

Bacterial glycolipids. Glycosyl diglycerides in gram-positive bacteria.

1. The lipids of ten Gram-positive bacteria have been isolated and the presence in each of a glycosyl diglyceride was established. 2. The glycolipid fractions were isolated and deacylated to give water-soluble glycosides which were purified by paper chromatography. Partial structures for the glycosides have been deduced from chemical and enzymic studies. 3. Nine of the glycosides were disaccharides glycosidically linked to the 1-position of glycerol: the remaining glycoside contained a trisaccharide similarly linked to glycerol.

Bacillus subtilis↗
PubMed1966

The specific substance from Pneumococcus type 34 the configuration of the glycosidic linkages.

1. The specific compound from Pneumococcus type 34 was isolated from capsular material by ion-exchange chromatography. This separated it from a substance with chemical and serological properties corresponding to those reported for C-substance. 2. The configuration of the two galactofuranosyl linkages in the repeating unit of S.34 was determined and the configurations previously assigned to the other glycosidic linkages were confirmed. 3. The dephosphorylated deacetylated repeating unit is thus O-beta-d-galactofuranosyl-(1-->3)-O-alpha-d-glucopyranosyl-(1-->2)-O-beta-d-galactofuranosyl-(1-->3)-O-alpha-d-galactopyranosyl- (1-->2)-ribitol.

Chromatography, Ion Exchange↗
PubMed1966

The type-specific substance from Pneumococcus type 10A(34). Structure of the dephosphorylated repeating unit.

1. A hexasaccharide unit was isolated from the specific substance S. 10A from Pneumococcus type 10A (34) by hydrolysis with alkali followed by enzymic dephosphorylation. 2. The hexasaccharide was shown to be O-d-galactofuranosyl-(1-->3)-O-d- galactopyranosyl-(1-->4)-O-[d-galactofuranosyl- (1-->6)]-O-2-acet-amido-2-deoxy-d-galactopyranosyl- (1-->3)-O-d-galactopyranosyl-(1-->2)-ribitol.

Chemical Phenomena↗
PubMed1965

The biosynthesis of streptomycin. The origin of the C-formyl group of streptose.

1. The biosynthesis of streptomycin in Streptomyces griseus has been studied by adding d-[3,4-(14)C(2)]glucose or d-[1,3-(14)C(2)]glucose to the growth medium and degrading the streptomycin produced. 2. The results suggest that the C-3' branch carbon atom of l-streptose arises from C-3 of d-glucose. 3. The mechanism of biosynthesis of streptose from glucose is discussed. It probably involves an intramolecular rearrangement of a 6-deoxy-4-oxyhexose derivative, and it is suggested that the nucleoside diphosphate sugar derivative hitherto recognized as an intermediate in the biosynthesis of l-rhamnose might participate in such a rearrangement.

Alcohols↗