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Biomedical subjects

J B Stern

Publications and source records attributed to J B Stern.

52 records · Page 3Linked to original sources
PubMed1988

Experimental chemotherapy-induced skin necrosis in swine. Mechanistic studies of anthracycline antibiotic toxicity and protection with a radical dimer compound.

The reactivity of antitumor anthracycline and mitomycin C antibiotics with the oxomorpholinyl radical dimers, bi(3,5,5-trimethyl-2-oxomorpholin-3-yl) (TM3) and bi(3,5-dimethyl-5-hydroxymethyl-2-oxomorpholin-3-yl) (DHM3), was studied in vitro. The oxomorpholinyl radical reduced daunorubicin to a quinone methide intermediate that reacted with solvent to form 7-deoxydaunorubicinone. The solvolysis reaction followed first order kinetics, and the reactivity rate constants (k2) measured for seven anthracycline analogues ranged from 2 X 10(-2) s-1 to 8.0 X 10(-4) s-1. The chemical reactivity of each anthracycline quinone methide correlated with the total skin toxicity caused by the respective parent anthracycline following injection into swine skin. Microscopic examination of experimental lesions in swine skin resemble those observed in humans after inadvertant chemotherapy extravasation. Hydrocortisone sodium succinate was not effective for the treatment of doxorubicin-induced skin necrosis, whereas DHM3 was effective for the treatment of skin necrosis caused by all seven anthracyclines and by the quinone containing antibiotic, mitomycin C.

Animals↗
PubMed1987

Dermatologic changes associated with interleukin 2 administration.

We have prospectively evaluated the skin changes that occurred in ten patients who were undergoing immunotherapy with interleukin 2 (IL-2) and autologous lymphokine-activated killer cells to treat cancer. Serial skin biopsy specimens were obtained before therapy (baseline), during IL-2 administration, and during IL-2/lymphokine-activated killer cell infusion. All patients developed an eruption that was characterized by macular erythema, with burning and pruritus of the skin. It began after two or three days of IL-2 infusion and was usually localized to the head and neck; it occasionally became generalized (ie, erythroderma). The eruption resolved with desquamation within 48 to 72 hours after cessation of infusion of IL-2. Histologically, the changes were not specific. The only consistent immunohistological finding noted was the presence of DR+/Leu-4+ lymphoid cells surrounding blood vessels in the papillary dermis, with fewer of these cells in the epidermis. There was no difference between the clinical or histological features of the eruption that occurred with IL-2 alone and that which occurred with IL-2 and lymphokine-activated killer cell infusion, suggesting that the cutaneous effects were mediated by IL-2 alone.

Adult↗
PubMed1986

Interleukin-2 induction of T-cell G1 progression and c-myb expression.

In studies to determine the biochemical mechanisms responsible for cell proliferation, synchronized T cells were used as a model for cellular growth control. By metabolic and morphologic criteria, it was found that activation of the T-cell antigen receptor rendered the cells responsive to interleukin-2 (IL-2), but did not move them through the cell cycle. Instead, IL-2 stimulated G1 progression to S phase, or lymphocyte "blastic transformation." During IL-2-promoted G1 progression, expression of the cellular proto-oncogene c-myb was induced transiently at six to seven times basal levels, maximal levels occurring at the midpoint of G1.

Animals↗
PubMed1985

Peptide products of the cleavage of bovine preprolactin by signal peptidase.

Cleavage of preprolactin (pPL) by detergent-solubilized signal peptidase produced mature prolactin and two small peptides derived from the signal peptide region of the pPL molecule. The production of both peptides was dependent on functional signal peptidase; the peptides were not generated at detergent concentrations that abolished signal peptidase activity. The amount of both peptides was proportional to the concentration of signal peptidase in the assay. The appearance of both peptides was insensitive to protease inhibitors, as was signal peptidase activity. The size, labeling characteristics, and amino acid sequence of the larger peptide, peptide 1, corresponded to those of the intact signal peptide of pPL. The smaller peptide, peptide 2, lacked the carboxy terminus of the signal peptide, and was, therefore, a fragment of intact signal peptide. These results demonstrate the endoproteolytic nature of signal peptidase.

Amino Acid Sequence↗
PubMed1985

Recurrent Spitz's nevi. A clinicopathologic investigation.

Spitz's nevi recur uncommonly following initial removal. A clinicopathologic investigation was performed on 16 such cases. The initial nevi in almost all cases extended to the margins of the sections. Otherwise, these lesions showed no unusual clinical or histologic features. The average recurrence time was 13 months, with a range from 2 months to 3 years. The recurrent lesions occasionally were mistaken clinically for keloids. Histologically, recurrent lesions were predominantly intradermal. Nests, fascicles, and individual spindle and/or epithelioid cells extended to the deep reticular dermis and occasionally into the subcutaneous fat. The surrounding stroma was densely fibrotic. Recurrent Spitz's nevi are different clinically and histologically from recurrent ordinary melanocytic nevi. They also often differ histologically and clinically from primary Spitz's nevi. Recurrent Spitz's nevi are similar histologically to the desmoplastic type of Spitz's nevus.

Adolescent↗
PubMed1984

Subcutaneous sacrococcygeal myxopapillary ependymoma. A clinicopathologic study of 32 cases.

A clinicopathologic investigation was performed on 32 cases of myxopapillary ependymoma occurring as a primary tumor of the skin and subcutaneous tissue of the sacrococcygeal area. The tumor usually presented as an asymptomatic mass at the base of the spine in young individuals. Pilonidal cyst was the most frequent clinical diagnosis. The excised tumors often "shelled out" easily and none was adherent to the vertebral column or spinal cord structures. The tumors were circumscribed and located in the subcutaneous tissue and deep dermis. Microscopically, ultramicroscopically, histochemically, and immunohistochemically, the cutaneous myxopapillary ependymomas showed distinct changes. Follow-up data were available for 23 patients with a median follow-up interval of 7 years. Six patients were followed for 15 years or longer. Metastases occurred in four patients, an incidence of 17%.

Adolescent↗
PubMed1984

Wells' syndrome: is there collagen damage in the flame figures?

Flame figures are a characteristic histologic feature of Wells' syndrome (eosinophilic cellulitis). In this light and electron microscopic study, the ultrastructure of flame figures is described. Collagen fibers were encrusted with free eosinophil granules. No collagen damage was found. Flame figures most probably represent a transient result of unusual eosinophil activity.

Aged↗
PubMed1981

Ultrastructure of subcutaneous sacrococcygeal myxopapillary ependymoma.

Electron microscopic examination was performed on three cases of myxopapillary ependymoma that occurred as primary tumors in the skin and subcutaneous tissue of the sacrococcygeal region. Some features found in both normal and neoplastic ependymal cells were observed. These included microvilli, cilia, desmosomal attachments, and cytoplasmic filaments. Other features noted, basement membranes and greatly decreased cilia, are characteristic of myxopapillary ependymomas and normal choroid plexus. In these situations, ependymal cells are apposed to connective tissue. The ependymal nature of this rare tumor is reaffirmed.

Basement Membrane↗
PubMed1980

Granular cell myoblastoma of the bronchus.

A granular cell myoblastoma of the bronchus occurring in a 20-year-old man was successfully treated by right lower lobectomy. His symptoms of chest pain and cough, the presence of an obstructing mass in a young patient, and the location of the lesion in a large bronchus on the right side are characteristic of this tumor. Electron microscopic examination of the tumor confirmed the diagnosis.

Adult↗
PubMed1979

Unusual benign bladder tumor of Brunn nest origin.

An unusual bladder tumor consisting of proliferating benign Brunn nests is described. The tumor occurred in a forty-five-year-old man who presented with hematuria. The tumor recurred in the same location one and one-half years later and again caused hematuria. The tumor is distinguished from inverted papilloma of the bladder.

Hematuria↗
PubMed1979

The edema of fiddler's neck.

Fiddler's neck is a dermatosis of violinists and violists. It characteristically presents as focal lichenification and pigmentation on the left side of the neck. Edema may also occur in the same area and lead to cosmetic concern or fear of malignancy. The edema apparently results from pressure on the patient's neck by the base of the violin or viola and is worsened by holding the instrument in a drooping position.

Dermatitis, Occupational↗