Biomedical subjects

J B Lynch

Publications and source records attributed to J B Lynch.

95 records · Page 6Linked to original sources

PubMedDate not supplied

Burns in alcohol and drug users result in longer treatment times with more complications.

Brezel, Kassenbrock, and Stein (J Burn Care Rehabil 1988;9:169-71), surveyed a group of 180 patients who were substance abusers or who were neurologically or mentally impaired and found that their inpatient care was more costly, more complicated, and more lengthy. In our institution we undertook a similar study to determine whether our findings would be similar. During a 12-month period, the charts of all patients over the age of 18 who were admitted for treatment of acute burns were reviewed. One hundred and thirty-one charts were available for study. Review of the charts revealed that 19 (14.5%) met our criterion of being impaired by drugs or alcohol, and the remaining 112 patients served as control subjects. The total body surface area burn averaged 25.8% in the impaired group and 21.3% in the control group. The amount of third-degree burns averaged 11% in the impaired group and 11% in the control group. Although the amount of third-degree burns was virtually identical in the two groups, the control group required an average of 1.2 procedures per patient, whereas the impaired group required 2.1 procedures per patient. A list of possible complications or adverse reactions that could occur was used to compare the two groups. The control group averaged 1.83 complications per patient, and the impaired group averaged 3.16 complications per patient. The average length of stay in the hospital for the control group was 19 days, and the average length of stay for the impaired group was 34.1 days.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMedDate not supplied

Progressive burn injury documented with vimentin immunostaining.

Precise determination of burn depth during the immediate postburn period remains an unresolved clinical problem. In an attempt to provide a new clinical option to aid in diagnosis of burn depth, an immunohistochemical marker (antivimentin) was used to examine excisional tissues or serial punch biopsies, or both, in partial-thickness human burn injuries. To test the hypothesis that burn injury continues to progress beyond the first 24 hours, burn depth was assessed by quantitative morphometric analysis in both a partial-thickness porcine burn model and in sequential samples from human patients. Vimentin immunostaining of ubiquitous mesenchymal populations resulted in a precise demarcation between burn eschar and the viable underlying dermis at 1 to 5 days after burn trauma. Porcine wounds showed continuous and significant progression in burn depth during days 1 through 3, but wounds were no deeper on the fourth postburn day. Similarly, 13 of 14 patients showed significant progression in burn depth between 1 to 5 days after burn injury. In conclusion, immunohistochemical staining with an antisera targeted toward a widely dispersed cell population in the dermis can be utilized as an effective tool to confirm the depth of tissue injury during the acute postburn period. Data from our randomly selected patients with partial-thickness burn suggest that burn wounds continue to demarcate for several days.

Adolescent↗

PubMedDate not supplied

Titanium tetrachloride: an unusual agent with the potential to create severe burns.

Titanium tetrachloride (TiCl4), an intermediate compound in the production of white pigment, can cause severe burns. Two cases are reported in which TiCl4 created 18% to 20% total body surface area burns. These full-thickness injuries were the combined consequence of hydrochloric acid and the heat that was generated in areas where this otherwise stable compound was mixed with perspiration. TiCl4 combined with water is extremely dangerous, and its immediate treatment--towel drying before irrigation--makes it unique among chemicals. Our experience suggests that in most cases grafting will be required. These chemical burns were self-limited and had no notable systemic sequelae. Wound biopsy specimens taken on postburn days 3 and 6 were subjected to immunostaining that showed that TiCl4 did not retard wound healing. Exposure time to TiCl4 vapor will determine the pulmonary and ophthalmologic involvement in each case. Clinical awareness of the propensity of TiCl4 to react with water--even when that water is in the form of perspiration--is vital because prompt management can limit the extent of injury.

Adult↗

PubMedDate not supplied

Antigen-specific cytokine responses in vaccinated Macaca nemestrina.

We describe a new surrogate assay for CD8 + T lymphocyte activity that has the capability of discriminating between cytotoxic T lymphocyte (CTL) activity and cytokine-mediated suppressive activity. We applied this approach to two groups of Macaca nemestrina vaccinated with a minimally pathogenic strain of human immunodeficiency virus type 2 [HIV-2 (HIV-2(KR))] as a model of an attenuated virus vaccine. Group 1 was then inoculated with a non-infectious stock of a pathogenic strain, HIV-2287. Both groups 1 and 2 were subsequently challenged with an infectious stock of HIV-2287. Five out of six group 1 animals were protected against CD4 decline, whereas three out of six animals in group 2 were protected. Analysis of CTL responses demonstrated strong activity against HIV-2(KR)-Gag in group 1. It was determined that strong CTL responses correlate with antigen-specific T-helper (Th) type 1 responses. This antigen-specific cytokine assay has the potential to better elucidate the functional mechanisms of CD8 + T-cell-mediated protection than traditional methods to date.

AIDS Vaccines↗

PubMedDate not supplied

Immunization against SIVmne in macaques using multigenic DNA vaccines.

All structural and regulatory genes of SIVmne were cloned into mammalian expression vectors to optimize expression in vitro and immunogenicity in mice. Macaca fascicularis were immunized four times with plasmid DNA (n = 4), or two DNA priming inoculations followed by two boosts of recombinant gp160 plus Gag-Pol particles (n = 4). Following intrarectal challenge with SIVmne, all macaques became infected. Three monkeys immunized with DNA alone maintained low plasma virus loads by 1 year post-challenge; the fourth exhibited high virus loads and significant CD4+ cell decline. Two of the DNA plus boost and three control macaques had high virus loads and associated CD4+ cell decline. Both vaccine protocols elicited antibodies and comparable helper T-cell proliferative responses to gp160. Cytokine mRNA levels in activated peripheral blood mononuclear cells (PBMC) taken at time of challenge suggested a dominant T helper (Th) 1 state in three DNA-immunized and one protein-boosted macaque, which correlated with low virus loads and high CD4+ cell counts post-challenge.

AIDS Vaccines↗