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Biomedical subjects

J B Hertz

Publications and source records attributed to J B Hertz.

At least 37 records · Page 2Linked to original sources
PubMed1982

Laparoscopic sterilization with the Falope-ring technique.

One hundred and sixteen women were admitted to hospital for laparoscopic sterilization by the Falope-ring technique. Up to the present, and with an average observation period of 20.8 months, no pregnancies have occurred. Bleeding from the distal site of puncture and tubal transection were the most common peroperative complications, occurring in 4.4 and 3.5% respectively. In 80 patients, hysterosalpingography was performed after 3 months as a control. Unilateral leakage was demonstrated in 6 patients; 5 of these were resterilized via a minilaparotomy. The Falope-ring technique is easy and quick to carry out and, when performed correctly, is encumbered with a low rate of complications and failures. The method is recommended as an alternative to sterilization via electrocautery of the tubes.

Adult↗
PubMed1981

Torsion of the fallopian tube.

Isolated torsion of the Fallopian tube is an uncommon condition. It occurs at all ages, though most frequently at the menstruating age, in normal as well as diseased tubes, in pregnancy and even after tubal sterilization. A cases is reported and the symptoms, differential diagnoses, etiology and diagnostic procedures are discussed. Early diagnosis and treatment is necessary if a twisted tube or a part of it is to be preserved; especially early laparoscopy may be a momentous diagnostic tool in these cases.

Adult↗
PubMed1981

Cross-reactions between Staphylococcus epidermidis and 23 other bacterial species.

By quantitative immunoelectrophoretic methods, 43 antigens were found in a mixture of sonicated preparations of four Staphylococcus epidermidis strains, using corresponding rabbit antiserum. Two of the antigens were identified as cell wall teichoic acid and a peptidoglycan antigen, respectively. Using this antigen/antibody reference system, cross-reactions between S. epidermidis antigens and antigens from other bacterial species were investigated. Fourteen of the S. epidermidis antigens cross-reacted with antigens from all S. aureus strains investigated. Only few cross-reactions were found between S. epidermidis and bacteria not belonging to the Micrococcaceae. The antigenic relatedness, expressed as a matching coefficient, seems promising for taxonomic work.

Antigens, Bacterial↗
PubMed1981

Quantitative studies on immunologically specific and non-specific absorption of Pseudomonas aeruginosa antibodies in serum from cystic fibrosis patients.

A quantitative determination of anti-Pseudomonas immunoglobulins was carried out by means of rocket-line immunoelectrophoresis in the serum from 19 cystic fibrosis (CF) patients with chronic P. aeruginosa infection and with many precipitins against these bacteria (CF + P). from six CF patients without P. aeruginosa infection (CF-P) and from nine normal persons. On an average P. aeruginosa antigens could absorb 7.7% of IgG, 8.4% of IgA and 29% of IgM from CF + P sera, whereas no detectable IgG and IgA and only 14.6% IgM were absorbed from normal sera and only 1.2% of IgG, 3.8% of IgA, but 29% of IgM was absorbed from CF-P sera. The results show that most, if not all, of the P. aeruginosa precipitins belong to the IgG and IgA classes, but that these precipitins can account only for part of the increased levels of immunoglobulins in CF-P patients. Staphylococcus aureus containing protein A (strain Cowan 1) could absorb 95% of the precipitating antibodies against P. aeruginosa and 92% of IgG, 27% of IgA and 34% of IgM in CF-P patients. The absorption of P. aeruginosa precipitins by protein A points to a possible synergism between S. aureus and P. aeruginosa infections in the lungs of CF patients, since S. aureus may interfere with antibody-mediated immune elimination of P. aeruginosa. Such a mechanism may also facilitate infections with other microorganisms in these patients.

Antibodies, Bacterial↗
PubMed1981

Abnormal prolactin levels and pituitary-gonadal axis in the puerperium.

To investigate the influence of breast-feeding and prolactin secretion on the pituitary-gonadal function, 3 different groups of patients were studied during the first 8 weeks of the puerperium. Group A comprised patients with hyperprolactinemia and secondary amenorrhea who conceived while on a regimen of bromocriptine. Group B was composed of normal lactating women. Group C comprised nonlactating women treated with bromocriptine. Group A patients had a normal decline in serum prolactin levels during the early postpartum period, but serum prolactin remained completely unaltered after clearance of placental estradiol. In group B suckling increased serum prolactin and suppressed luteinizing hormone. This pattern was not seen in group A. Group C patients had a rapid postpartum normalization of the pituitary-gonadal axis. The results indicate that in relation to lactation the pituitary function is rather autonomous in hyperprolactinemic patients.

Adult↗
PubMed1980

Threatened abortion studied by estradiol-17 beta in serum and ultrasound.

One hundred women were admitted to the hospital between 7 and 18 weeks' gestation because of vaginal bleeding and threatened abortion. Serum concentration of estradiol-17 beta was determined by radioimmunoassay. Ultrasound examination using a gray-scale scanner was performed as well. The values for prediction of abortion or successful outcome of pregnancy were calculated for estradiol (E2) and ultrasound separately and in combination. Prediction of abortion from a low E2 concentration combined with an ultrasound examination showing no fetal life was found to be totally reliable. Prediction of successful outcome of pregnancy from either a normal E2 or an ultrasound examination showing fetal life, or both, was 79.5% reliable. If no signs of fetal life were found by ultrasound examination from 10 weeks' gestation on, the pregnancy was doomed. Before this gestational age, measurements of E2 in serum gave supplementary information concerning the outcome of the threatened pregnancy.

Abortion, Threatened↗
PubMed1980

Antibody response in patients with Pseudomonas aeruginosa infection to a 'common antigen' from P. aeruginosa analysed by means of quantitative immunoelectrophoretic methods.

381 sera from 101 patients suffering from Pseudomonas aeruginosa infection have been investigated for antibodies against a 'common antigen' (CA) and other antigens from P. aeruginosa by means of crossed immunoelectrophoresis with intermediate gel, using a polyspecific P. aeruginosa antigen/antibody reference system. The earliest antibody response during the P. aeruginosa infections included increased CA antibodies in only 38% of the patients examined. The peak level of the antibody response included increased CA antibodies in 71% of the patients. By means of 'absorption of antibodies in situ', it was found that the antibody response to CA was directed against both the common cross-reactive part of the CA molecule and the Pseudomonas-specific part, with individual variations. During infections caused by other bacteria containing CA, one-third of the patients developed antibodies against CA. The diagnostic implications of the results are discussed.

Antibodies, Bacterial↗
PubMed1980

An antigen common to a wide range of bacteria. I. The isolation of a 'common antigen' from Pseudomonas aeruginosa.

In crude water-soluble extracts of Pseudomonas aeruginosa 64 antigens can be demonstrated by crossed immunoelectrophoresis in agarose with polyvalent Pseudomonas-immunoglobulin. One of these antigens cross-reacts with antigens prepared from bacteria of a wide range of taxonomic groups. Monospecific immunoglobulins to this antigen (Common Antigen) were produced by immunization with the appropriate immunocomplex extracted from agarose. Common Antigen was purified by the combination of two fractionation methods: Precipitation of the crude extract with 18% (w/v) sodium sulfate, followed by gel filtration of the supernatant on a Sephadex G-200 column. By this method, 35% of Common Antigen from the crude extract was recovered, more than half of the fractions electrophoretically pure. Electrophoresis of reduced Common Antigen on a dodecyl sodium sulfate polyacrylamide gel revealed two protein bands with apparent molecular weights of 59-62 000 and 62-65 000, respectively. The untreated antigen, however, passed a column of Sephadex G-200 with the void volumen, indicating a substance of high molecular weight (> 4-600 000).

Antigens, Bacterial↗
PubMed1980

Quantitative immunoelectrophoretic analysis of Salmonella typhi antigens and of corresponding antibodies in human sera.

By quantitative immunoelectrophoretic methods, 86 different antigens were found in sonicated preparations of a Salmonella typhi, using corresponding rabbit antiserum. Lipopolysaccharide was identified, and 5 other antigens characterized. Antibodies against a total of 19 S. typhi antigens were found in human sera. The antibody response in patients with typhoid fever was significantly more pronounced compared to that in normal persons. When the antibody response was expressed as a precipitin score, the predictive value of both a positive (precipitin score greater than or equal to 8) test and a negative test was 100%, when sera from patients suffering from typhoid fever were compared with sera from normal persons.

Animals↗
PubMed1980

Cross-reactions between Salmonella typhi and 24 other bacterial species.

Cross-reactions between antigens from S. typhi and 24 other bacterial species were studied by quantitative immunoelectrophoretic methods. A sonicated S. typhi antigen preparation and a corresponding pooled rabbit antiserum regularly presenting 86 immunoprecipitates were used as a reference system. Antigens from Enterobacteriaceae cross-reacted intensively with the S. typhi antigens. No cross-reactions between S. typhi and antigens from gram-positive bacteria were found, and only a few cross-reactions with antigens from gram-negative bacteria outside the Enterobacteriaceae. The taxonomic perspectives of the immunoelectrophoretic approach are stressed by results showing that the number of cross-reacting antigens between S. typhi and other Enterobacteriaceae was positively correlated to the number of identical reactions in a collection of 28 biochemical tests.

Antigens, Bacterial↗
PubMed1980

An antigen common to a wide range of bacteria. 2. A biochemical study of a "common antigen" from Pseudomonas aeruginosa.

Common Antigen (CA) of Pseudomonas aeruginosa has been shown to be a protein composed of polypeptide subunits of a molecular weight (MW) of about 62 000. The MW of this protein was estimated to 665 000 by gel filtration on sepharose CL-6B, to 800 000 by electrophoresis on polyacrylamide gradient gels and to about 900 000 by ultracentrifugation, on a sucrose gradient. By analytical ultracentrifugation with Schlieren optics a sedimentation coefficient (S20 degrees, W) of 22.65 was calculated. The isoelectrical point was determined to pH 4.4. The antigen was decomposed on exposure to proteolytic enzymes. Polysaccharide, lipid, deoxyribonucleic acid or ribonucleic acid were not demonstrated in CA. The amino acid content of CA was determined, and no hexosamine or abnormal residues were observed. The amino acid content of CA was determined, and no hexosamine or abnormal residues were observed. The antigen was degraded when heated to 100 degrees C for 4 min or when exposed to pH below 4 or above 11 at 4 degree C. CA has been isolated from the cytoplasmic water-soluble fraction of disintegrated bacteria and only trace-amounts could be obtained from envelope fractions after solubilization with Triton X-100.

Amino Acids↗
PubMed1980

Progesterone and human chorionic gonadotrophin in serum and pregnandiol in urine in threatened abortion.

Progesterone and human chorionic gonadotrophin (HCG) in serum and pregnandiol in urine were measured in 64 patients admitted to hospital because of threatened abortion. Blood samples were taken and urine specimens collected at regular intervals during admission and after discharge during the rest of the pregnancy. A reference range was worked out for each hormone based on the hormone values obtained from the pregnancies proceeding to term. The predictive significance of values within and below the reference range was determined, for the initial sample and for serial samples. An association between hormone levels and outcome of pregnancy was observed but it is concluded that both single and serial determinations of progesterone and pregnandiol and serial determinations of HCG are unsatisfactory for the evaluation of threatened abortion. However, an initial progesterones value below the reference range and HCG values below 10,000 mIU/ml between the 8th and 15th week of pregnancy was in every case always followed by spontaneous abortion. A hormonal test of fetoplacental origin is recommended for monitoring threatened abortion.

Abortion, Threatened↗
PubMed1979

Cross-reactions between Staphylococcus aureus and fifteen other bacterial species.

Fifty-five different antigens were demonstrated in an antigen preparation obtained by sonication of a protein A-free strain of Staphylococcus aureus, using crossed immunoelectrophoresis and antiserum obtained from rabbits. The antigens were characterized by absorption experiments with formalin-killed bacterial cells, temperature resistance and protein and polysaccharide staining. One of the antigens showed reaction of identity with a preparation of teichoic acid from S. aureus. Using this reference system, cross-reactions between the S. aureus reference strain and S. aureus strains from the four phage groups as well as 15 other bacterial species, were studied by various quantitative immunoelectrophoretic methods. The S. aureus antigens from the four phage groups showed almost 100% cross-reactivity for all antigens, while antigens from nine other bacterial species cross-reacted to varying degrees with one to four S. aureus antigens. All of 12 S. aureus antigens cross-reacted with the four S. epidermidis biotypes.

Antigens, Bacterial↗