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J B Graham

Publications and source records attributed to J B Graham.

At least 73 records · Page 4Linked to original sources
PubMed1983

Permanent cell line expressing human factor VIII-related antigen established by hybridization.

A permanent human cell line, EA . hy 926, has been established that expresses at least one highly differentiated function of vascular endothelium, factor VIII-related antigen. This line was derived by fusing human umbilical vein endothelial cells with the permanent human cell line A549. Hybrid cells that survived in selective medium had more chromosomes than either progenitor cell type and included a marker chromosome from the A549 line. Factor VIII-related antigen can be identified intracellularly in the hybrids by immunofluorescence and accumulates in the culture fluid. Expression of factor VIII-related antigen by these hybrid cells has been maintained for more than 100 cumulative population doublings, including more than 50 passages and three cloning steps. This is evidence that EA . hy 926 represents a permanent line.

Antigens↗
PubMed1981

Conservative management of twisted testicular appendages.

We planned non-operative treatment of a twisted intrascrotal appendage in 23 consecutive patients, ranging in age from 6 to 45 years, in whom the diagnosis could be made confidently. Only 3 patients have required subsequent removal of the infarcted appendage because of persistent or recurrent pain. The remaining patients became free of pain within a week. Prompt surgical exploration to exclude torsion of the spermatic cord remains necessary if scrotal swelling obscures the diagnosis. At operation for the latter indication 5 additional boys were found to have a twisted appendix testis during the study interval.

Adolescent↗
PubMed1980

Genetic analysis of von Willebrand's disease in two large pedigrees: a multivariate approach.

Clinical and laboratory data, including polymorphic marker traits for linkage analysis, were collected from two large multigenerational families segregating for von Willebrand disease. A new approach to the identification of gene carriers in these families, combining pedigree segregation analysis with multivariate discriminant analysis, is applied. Whereas individually the clinical symptoms and the factor VIII related activities could not distinguish between hypotheses, it was possible to find a discriminant function-showing consistency of the data with a dominant gene hypothesis, but not with a recessive gene or an environmental hypothesis. This function is estimated to lead to 3.2% and 5.5% minimum misclassification of the genotypes, respectively, in the two families. The discriminant function could be used for other families, but is should be calibrated for the specific population in which it is used. Among the markers investigated, GPT is the most likely to be linked to von Willbrand's disease, with a maximum lod score of about unity at 15% recombination.

Factor VIII↗
PubMed1980

Cavernous hepatic hemangioma simulating metastatic renal cell carcinoma.

A case of cavernous hemangioma of the liver resembling a metastatic deposit is reported in a patient with renal cell carcinoma. The clinical and angiographic features that distinguish hepatic hemangiomas from metastatic renal cell carcinoma are discussed. The importance of this distinction in patient management is emphasized.

Adenocarcinoma↗
PubMed1980

Endothelial cell hybrids and the suspension of factor VIII related antigen expression.

Hybrid somatic cell clones have been generated by fusing human vascular endothelial cells in primary culture to cells of four rodent lines. Factor VIII related antigen (VIIIR:Ag) was clearly demonstrable in the cultured endothelial cells, even when they had been co-cultured with rodent cells. But in none of 14 hybrid clones was VIIIR:Ag detectable. Isozyme analyses for human chromosome markers show that all the assayed human chromosomes were represented among the hybrids, and that various subsets of human chromosomes have been deleted from individual hybrid clones. It may be concluded, therefore, either that VIIIR:Ag production depends on a particular combination of human chromosomes not represented in any of the hybrids, or that the rodent cells contribute some agent which intracellularly blocks VIIIR:Ag expression.

Antigens↗
PubMed1980

Identifying carriers of mild haemophilia.

The problems of carrier identification in mild haemophilia were examined by studying a large kindred transmitting VIII:C levels which average 17 u/dl in affected males. Fifteen obligatory carriers and 13 normal women from this kindred were used as reference groups to produce a set of linear discriminants. The utility of these discriminants in identifying carriers of mild haemophilia was compared with that of a similar set of discriminants which had been prepared for use with carriers of severe haemophilia, each set of discriminants being tested against both data bases. It was found that both sets of discriminants had large error rates when applied to the mild data base, and that both had much smaller error rates when applied to the severe data base. This outcome resulted from the greater overlap between the factor VII-related activities of mild carriers and normal women than the overlap of these activities between severe carriers and normal women. Our conclusions were that (i) correct classification of many but not all women from families of mild haemophilia would be difficult, (ii) discriminants prepared using obligatory carriers of severe haemophilia were not more inefficient in mild haemophilia than those prepared using obligatory carriers of mild haemophilia, (iii) a single set of discriminants prepared from reference groups of normal women and obligatory carriers of severe haemophilia may be used for identifying potential carriers of all grades of haemophilia, and (iv), statistically speaking, VIIIR:Ag assays provide a limited amount of information for this purpose.

Age Factors↗
PubMed1980

Factor VIII coagulant antigen in hemophilic plasma: a comparison of five alloantibodies.

Five independent alloantibodies directed to factor VIII coagulant antigen (VIII:CAg) were assessed against normal, von Willebrand's disease, and severe hemophilia A plasmas. Immunoradiometric assays (IRMAs) were developed for each antibody, one of which had arisen "spontaneously" and four in transfused hemophiliacs. The correlations between assays were very high for normal, vWd, and both CRM+ and CRM- hemophiliacs. This suggests that IRMAs maybe developed from almost any reasonably high titered alloantibody and used with confidence in diagnosing CRM- hemophilia A in utero by fetoscopy.

Antibody Specificity↗
PubMed1979

The separation of Willebrand factor from factor VIII-related antigen.

The three activities associated with factor VIII--coagulant (VIII:C), antigenic (VIIIR:Ag), and platelet agglutinating or Willebrand factor (VIIIR:WF)--have been separated by sequential antibody affinity chromatography, utilizing a rabbit antibody to factor VIII and a spontaneous human antibody to VIII:C. Normal plasma differentially lost its factor VIII-related antigen following passage over the rabbit antibody column. Subsequent passage of the VIIIR:Ag-depleted plasma over the human antibody column resulted in the loss of VIII:C activity, with retention of the Willebrand factor activity, antigen being partially recovered from the heterologous antibody column. These experiments demonstrate that it is possible to separate two of the factor VIII activities, VIIIR:Ag and VIIIR:WF, which are usually regarded as properties of a single molecule.

Antibody Affinity↗
PubMed1979

Genetics of classic von Willebrand's disease. II. Optimal assignment of the heterozygous genotype (diagnosis) by discriminant analysis.

In classic von Willebrand's disease (vWd), assignment of the heterozygous genotype for genetic studies and diagnosis for clinical purposes (which are not exactly the same) are formidable problems. We have pointed out in the first report in this series that almost 50% of the members of two large kindred who transmitted this disease, and were therefore heterozygous, were scored as normal by the usual tests of hemostasis. This report describes how this large proportion can be significantly reduced by application of discriminant analysis. Using linear discriminants in three variables--coagulation factor VIII (VIII:C), factor-VII-related antigen (VIIIR:Ag), and the ristocetin cofactor related to factor VIII (VIIIR:WF)--we were able to classify as heterozygous more than 80% of the transmitters in the two large kindred. It was of particular interest that the four parents of two related vWd homozygotes could be scored as heterozygous by discriminant analysis even though all their laboratory tests were within the normal ranges.

Adult↗