Search PubMed⌕ Search

Biomedical subjects

J Augustine

Publications and source records attributed to J Augustine.

40 records · Page 3Linked to original sources

Bed mobility task performance in older adults.

Difficulty in transferring, the ability to rise in and out of a bed and chair, is a common problem in older adults, particularly those residing in skilled nursing facilities. Focusing on one aspect of transferring, rising from supine to sitting position, we devised a set of bed mobility tasks to test key arm, leg, and trunk movements that likely contribute to successful rising from bed. Healthy young controls (YC, n=22, mean age 23), and older adults (aged 60 and over) either residing independently in congregate housing (CH, n=29, mean age 84) or undergoing rehabilitation in a skilled nursing facility (SNF, mean age 77) were assessed in the time to rise from supine to sitting and in the ability or inability to perform 16 other bed mobility tasks. Trunk function-related tasks, specifically those requiring trunk elevation and trunk balance, were most difficult for the SNF, followed by CH, and then YC. Tasks focusing on trunk flexion strength (sit up arms crossed, bilateral heel raise) and lateral trunk strength/balance were the most difficult for both SNF and CH, although there was minimal difference in the percent unable to complete each task. The major CH-SNF differences occurred in trunk elevation tasks where the upper limb was important in facilitating trunk elevation (sit up with head of bed elevation with use of arms, sit up with the use of a trapeze, or sit up with use of arms from a flat bed position). These findings suggest that to improve frail older adult performance on bed mobility tasks, and specifically in rising from supine to sitting, training should move beyond improving trunk function (i.e., trunk strength). There should be an additional focus, either through therapy or bed design modifications, on how upper limb movements and positioning can be used to assist in trunk elevation.

Activities of Daily Living↗

Jack fruit lectin binding pattern in the exfoliative cytology of bronchopulmonary neoplasia.

N-acetyl D- galactosamine specific jack fruit lectin conjugate was used for the cyto-chemical study of benign and neoplastic lesions of the respiratory tract using diaminobenzidine as substrate on exfoliated cells and tissue sections. Sputum samples from patients with benign respiratory tract lesions (40), squamous cell Carcinoma (20), adenocarcinoma (16), small cell anaplastic carcinoma (12) and large cell anaplastic carcinoma (5) were used for the study. The lectin binding was strong in squamous cell carcinoma, large cell anaplastic carcinoma and adenocarcinoma, but variations in the binding pattern were observed in different carcinoma. Squamous metaplastic cells manifested slightly increased binding to lectin as compared to bronchial and native squamous epithelial cells. The nature of binding was eventually similar in cytology and histology. The ready availability and ease of preparation in purified from makes the jack fruit lectin a potential cytochemical reagent for sputum cytology.

3,3'-Diaminobenzidine↗

Neonatal autoperfused working heart-lung preparation: assessment of factors determining survival.

The function and survival of the autoperfused working heart-lung preparation in the newborn period was studied in six newborn pigs (3 to 5 days old). Normothermic autoperfusion was maintained by a closed blood-primed circuit. Left ventricular afterload was determined by positioning the blood reservoir, and the venous return was adjusted to maintain a right atrial pressure of 6 to 8 mm Hg. Left ventricular function was assessed by a conductance catheter in the left ventricle and was determined by the slope of the end-systolic pressure-volume relationship. Left ventricular diastolic compliance was measured from the end-diastolic pressure-volume relationship. Serial measurements at 60, 120, 180, and 240 minutes showed no change in the end-systolic pressure-volume relationship, whereas there was a significant leftward shift of the end-diastolic pressure-volume relationship after 240 minutes (0.42 +/- 0.02 ml-1 to 0.66 +/- 0.04 ml-1, p less than 0.05). Cessation of effective cardiac function occurred at 268 +/- 10 minutes (+/- SEM). This followed progressive deterioration in pulmonary function based on measurement of arterial blood gases and peak airway resistance. This study shows the feasibility of maintaining systolic cardiac function in a newborn autoperfused working heart-lung preparation. Extended survival beyond 4 hours will require improved methods to preserve left ventricular diastolic function and especially pulmonary function.

Animals↗

Jack fruit lectin binding pattern in benign and malignant lesions of the breast.

N-acetyl D-galactosamine specific lectin was isolated from Jack fruit (Artocarpus integrifolia) and conjugated to horse radish peroxidase type VI. The purified conjugate was used for the study of tissue binding properties on benign and malignant lesions of the breast using diaminobenzidine as substrate on dewaxed tissue sections. Forty mammary carcinomas, 10 cystic hyperplasias of the breast and 10 normal breast tissues were used for the study. Neoplastic cells showed increased affinity to the lectin. The lectin binding was focally strong in neoplastic cells compared to the normal as well as the hyperplastic tissues. The stroma of the cancer tissues showed an intense strong binding where elastosis was present. The use of the lectin as a histochemical reagent is discussed.

Acetylgalactosamine↗