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Biomedical subjects

J Asakawa

Publications and source records attributed to J Asakawa.

42 records · Page 3Linked to original sources
PubMed1984

Electrophoretic variants of blood proteins in Japanese. III. Triosephosphate isomerase.

A total of 15,387 individuals living in Hiroshima and Nagasaki, of whom 10,864 are unrelated, were examined for erythrocyte triosephosphate isomerase (TPI) by starch gel electrophoresis using TEMM buffer, pH 7.4. Four kinds of new variants, one having a cathodal migration and three having anodal migrations, were encountered in this population. These variants were further characterized by starch gel electrophoresis using tris-EDTA buffer, pH 9.3, and isoelectric focusing. An anodally migrating allozyme TPI 2HR1 exhibited markedly decreased enzyme activity, as evaluated by the staining intensity of the variant bands. The level of TPI activity in erythrocytes from this individual with the phenotype TPI 1-2HR1 was about 60% of the normal mean. Family studies confirmed the genetic nature of all the variants.

Carbohydrate Epimerases↗
PubMed1982

Characterization of two new electrophoretic variants of human triosephosphate isomerase: stability, kinetic, and immunological properties.

Two new electrophoretic variants of human triosephosphate isomerase (TPI) have been partially purified and characterized. The TPI Manchester variant, a cathodally migrating electrophoretic allozyme identified in an individual with the phenotype TPI 1-Manchester, is associated with a normal level of enzyme activity in erythrocytes and normal kinetic properties. It is very thermolabile at 55 and 57 degrees C, although it is not uniquely sensitive to either guanidine-HCl or urea denaturation. The TPI Hiroshima-2 variant is an anodally migrating allozyme (the phenotype of proband is TPI 1-Hiroshima-2) with normal activity and kinetic properties and also normal stability characteristics. It is inactivated less by antisera raised against normal human TPI than either the normal or the Manchester allozyme. Dissociation-reassociation experiments utilizing these allozymes have confirmed that normal human red blood cell TPI isozymes are produced by a sequence of reactions (presumably deamidations) involving alternating subunits.

Antigen-Antibody Reactions↗
PubMed1980

Search for mutations affecting protein structure in children of atomic bomb survivors: preliminary report.

A total of 289,868 locus tests, based on 28 different protein phenotypes and using one-dimensional electrophoresis to detect variant proteins, has yielded one probable mutation in the offspring of "proximally exposed" parents, who received an estimated average gonadal exposure of 31 to 39 rem in the atomic bombings of Hiroshima and Nagasaki. There were no mutations in 208,196 locus tests involving children of "distally exposed" parents, who had essentially no radiation exposure.

Dose-Response Relationship, Radiation↗
PubMed1980

Variants of red cell enzymes found in Japanese of Hiroshima and Nagasaki.

In the course of our search for possible genetic effects of radiation among the children of A-bomb survivors in Hiroshima and Nagasaki, we have obtained considerable electrophoretic data which clearly illustrate some of the genetic characteristics of the populations of both cities, which characteristics, however, have no relationship with radiation exposure of the parents. The frequencies of variants of phosphoglucomutase1 (PGM1) and glucose phosphate isomerase (GPI) were observed to be significantly higher in Nagasaki than in Hiroshima. As the difference in the frequencies of transferrin variants between two cities are also significant, it is apparent that the populations of the two cities are genetically different with respect to variation in certain protein systems.

Child↗