Mucosa-associated lymphoid tissue (MALT) lymphoma.
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Humans↗
Biomedical subjects
J Asai
Publications and source records attributed to J Asai.
Effectiveness of Carolina rinse solution after cold ischemic storage of rat livers: biochemical and histological analysis using perfusion model.
Using a rat liver perfusion model, the effectiveness of Carolina rinse solution was assessed for the prevention of reperfusion injury after 48 h of cold storage in UW solution. Transaminase levels (GOT, GPT and LDH) of the perfusate were significantly higher in the Ringer group (17 +/- 8, 17 +/- 9 and 191 +/- 97 IU/l, respectively) than in the Carolina group (6 +/- 4, 5 +/- 4 and 21 +/- 20 IU/l) (p < 0.05). The levels of oxygen consumption were also higher in the Carolina group (233 +/- 54 mm Hg) than in the Ringer group (164 +/- 58 mm Hg) (p < 0.05). Histological examination showed severe parenchymal cell damage in the Ringer group, whereas the damage was slight in the Carolina group. Two newly developed monoclonal antibodies, REC16-11 and REC4-1, which specifically react with rat endothelial cells, were used for immunohistochemical studies of the livers. The endothelial cells of central vein and sinusoids were more severely damaged in the Ringer group than in the Carolina group. The present study suggests that Carolina rinse solution is useful for prevention of liver damage from reperfusion injury after cold storage of the graft for organ transplantation.
Animals↗
Fatal Epstein-Barr virus-associated lymphoproliferative disorder in childhood.
OBJECTIVE: We report five childhood cases of fatal Epstein-Barr virus-associated lymphoproliferative disorder to determine their immunopathologic and immunogenotypic features. DESIGN: Clinicopathologic features are described, using clinical, histologic, immunophenotypic, and genotypic examinations. SETTING: Autopsy cases were performed at Nagoya and Tsukuba university hospitals, Japan. RESULTS: Infiltrating lymphocytes, which were positive for the EBV genome by in situ hybridization, were polymorphic and showed polyclonal immunoglobulin staining in all five cases. Two cases, however, showed genetic clonality of EBV termini. In one case, the clonality was observed only in the spleen, and in the other case, clonal rearrangement of the JH gene was also found. The former case showed none of the morphologic features of neoplasia and presented a morphology similar to virus-associated hemophagocytic syndrome. In the latter case, infiltrating lymphoid cells were much less polymorphous, and nodular masses of the lymphoid cells in colon and lung specimens were observed. CONCLUSION: We suggest that some polyclonal EBV-associated lymphoproliferative disorders develop into polymorphic, but genotypically monoclonal, lymphoproliferative disorders.
Adolescent↗
Primary gastric Hodgkin's disease. Morphologic, immunohistochemical, and immunogenetic analyses.
Two cases of primary gastric Hodgkin's disease were examined morphologically and immunohistochemically. Immunogenetic studies were also made in one of the cases. Case 1 was diagnosed as nodular sclerosis and case 2 as mixed cellularity Hodgkin's disease. Large atypical mononuclear or multinucleated giant cells were immunohistochemically positive for BerH2, whereas they were negative for LCA, L26, DAKO CD3, MT1, UCHL-1, S100 protein, and MAC387. In case 1, large atypical cells examined immunohistochemically on frozen tissue specimens were positive for Ki-1 and negative for T- and B-cell markers. Southern blot hybridization studies performed on material from case 1 revealed germline configuration of immunoglobulin JH and T-cell-receptor beta-chain genes. To our knowledge, this is the first report in which primary gastric Hodgkin's disease has been studied immunogenetically. Although rare in incidence, the existence of Hodgkin's disease in the stomach must be noted.
Aged↗
Simultaneous measurement of the ( gamma,p)/( gamma,n) cross-section ratio in 4He as a test of charge symmetry.
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Journal Article↗
Kinetics of intestinal intraepithelial lymphocytes during acute graft-versus-host disease in mice.
We examined the kinetics of intestinal intraepithelial lymphocytes (IEL) and the incidence of apoptosis at villus or crypt sites during the development of non-irradiated acute graft-versus-host disease (GVHD). The first IEL to increase were host-origin on day 3 and the donor-derived IEL appeared first on day 12 after GVHD induction. Unique CD3+CD4+CD8 alpha/alpha+ IEL were significantly increased on day 6 and an appreciable number of IEL bearing T cell receptor V beta capable of recognizing self-superantigen were detected on day 9. The sudden appearance of apoptosis and reduction of mitotic activity occurred on day 12, accompanied by a dramatic decrease of CD3+CD4-CD8 alpha/alpha+ IEL of host origin. CD8 alpha/alpha+ IEL of host origin, which expand and then decrease by apoptosis at the early stage of acute GVHD, may be associated with pathogenesis of the enteropathy occurring during acute GVHD.
Animals↗
3He( gamma,pd) cross sections with tagged photons below the Delta resonance.
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Journal Article↗
Primary gastric Ki-1 positive anaplastic large cell lymphoma: a report of two cases.
Two cases with primary gastric Ki-1 positive anaplastic large cell lymphoma are presented. Morphologic features of both cases involved pleomorphism of the neoplastic cells, fibrosis and lymphatic infiltration. The neoplastic cells in both cases were positive for BerH2 (CD30), LCA(CD45), lysozyme and alpha-1-antitrypsin (alpha 1-AT). In additional case, the neoplastic cells were additionally positive for MAC387 and alpha 1-antichymotrypsin (alpha 1-ACT). The neoplastic cells in these cases were negative for L26(CD20), UCHL-1(CD45RO), DAKO CD3 and epithelial membrane antigen (EMA). According to the results of the phenotypic studies, the authors consider that the neoplastic cells have some of the features of histiocytes. Both patients at 2 and 8 years after surgery without chemotherapy are disease free. This lymphoma is well known to be frequently misdiagnosed as undifferentiated carcinoma. Although rare in occurrence, recognition of this primary lymphoma in the stomach has a significant clinical implication, as the authors consider that its prognosis might be better than undifferentiated carcinoma of the stomach.
Adult↗
Basic fibroblast growth factor-binding domain of heparan sulfate in the human glomerulosclerosis and renal tubulointerstitial fibrosis.
BACKGROUND: The saccharide side chains of heparan sulfate (HS) proteoglycans show enormous complexity. These polysaccharides can interact specifically with cytokines such as basic fibroblast growth factor (bFGF). The understanding of HS expression in glomerulosclerosis and interstitial fibrosis, which is still rudimentary, could provide some insight about the role of bFGF in kidney diseases. EXPERIMENTAL DESIGN: Kidney sections were exposed to exogenous bFGF and then to a monoclonal anti-bFGF antibody. Specificity of the interaction between HS and bFGF was established by monitoring concomitant loss of bFGF during selective removal of HS with heparitinase and competitive inhibition studies. To further characterize regional changes in saccharide sequences, heparitinase-generated unsaturated disaccharides, N-sulfated glucosamine-enriched but O-sulfate-scarce portions characteristics of native HS, and such portions characteristic of Engelbreth-Holm-Swarm tumor HS were studied. RESULTS: HS was detected in interstitial fibrosis and in advanced glomerulosclerosis, whereas bFGF-binding domains were found only in the fibrosis: The distributional pattern of the N-sulfate-enriched and O-sulfate-scarce portions of native HS was similar to that of bFGF-binding domains. Moreover, a small population of parenchymal cells in advanced tubulointerstitial fibrosis with marked cellular infiltration were especially rich in the bFGF-binding domains. CONCLUSIONS: In fibrotic lesions of the peritubular interstitium, HS shows enrichment of bFGF-binding domains. These regions may play an important role in the fibrogenesis through their interaction with endogenous bFGF.
Adult↗
Natural killer cell leukemia. An autopsy case.
We describe an autopsy case of natural killer cell leukemia. Leukemic cells appeared morphologically as large granular lymphocytes and expressed a restricted natural killer cell phenotype (CD2, CD38, CD56, HLA-DR). Immunogenotypic analysis revealed germline configurations of TCR-beta, TCR-gamma, and JH genes, indicating a non-T-cell or non-B-cell lineage. These features are consistent with natural killer cell leukemia, a recently proposed clinicopathologic entity. Autopsy findings were characterized by an extremely dense infiltration of leukemic cells in most organs, along with destruction of the normal architecture, corresponding to an aggressive clinical course. Hemophagocytic histiocytosis and an absence of chronic inflammatory reactions caused by recurrent infection in our case are different from morphologic features of CD3+ large granular lymphocytic leukemia. No relationship between Epstein-Barr virus and natural killer cell leukemia was observed.
Female↗
Primary gastric T-cell lymphoma. Morphological and immunohistochemical studies of two cases.
Primary T-cell lymphoma of the stomach is very rare. We report two cases of primary gastric T-cell lymphoma, one of which had a morphological appearance and a phenotype that were similar to those of node-based peripheral T-cell lymphoma. The other case was related to human T-cell lymphotropic virus type I and displayed a feature of lymphoma cell pleomorphism. The lymphoma cells were both positive for Ki-1/CD30.
Blotting, Southern↗
A case with primary gastric lymphoma producing IgM and IgG immunoglobulins.
We describe a primary gastric lymphoma with IgM kappa and IgG kappa types of immunoglobulin in the neoplastic cells. The neoplastic cells containing IgM were morphologically different from those containing IgG. The IgG-bearing cells had globular cytoplasmic inclusions, but IgM-bearing cells did not. The neoplastic cells had two Ig heavy-chain gene rearrangements, with a germline band and a single kappa light-chain gene rearrangement. Immunoglobulin expression with light-chain restriction of the neoplastic cells and single light-chain gene rearrangement indicated that the morphologically different neoplastic cells were derived from a common progenitor. The expression of IgM and IgG and two rearrangement bands of immunoglobulin heavy-chain gene suggested that class switching might have occurred in the lymphoma cells.
Aged↗
Immunohistochemical and functional studies on rat high endothelial venules using monoclonal antibodies.
The distribution of high endothelial venules (HEVs) in various rat organs has been investigated immunohistochemically using two monoclonal antibodies (MoAbs), REC16-11 and REC4-1. The staining patterns observed with REC16-11 and REC4-1 were compared with those obtained with the MoAb anti-rat ICAM-1, which is a cell adhesion molecule in HEVs. REC16-11 reacted not only with HEVs but also with all vascular endothelial cells (ECs) in the tissues examined. Electronmicroscopy showed that REC16-11 and REC4-1 reacted with the cell surface antigens of ECs and immunoblot analysis of rat splenic stromal preparations showed that REC4-1 stained 42 kd and 60 kd bands. REC4-1 inhibited the binding of lymphocyte to HEVs in the mucosa-associated lymphoid tissues (MALT), but had no effects on lymphocyte binding to HEVs in peripheral (non-mucosal) lymph nodes. These findings suggested that the MoAb REC4-1 recognized the associated antigen of the lymphocyte-HEV recognition system in MALT.
Animals↗
Thy-1 antigen dendritic cells and rejection of composite tissue limb allografts in rats.
Dendritic-shaped cells (Thy-1+DC) characterized by the surface phenotype of Thy-1+, asialo GM1+, Ia- are reported to exist exclusively in mice. However, Thy-1+ dendritic cells were observed in the skin of normal athymic nude F344, euthymic F344, and WKAH rats. Their phenotypic markers were Thy-1+, asialo GM1+, Ia-, W3/25 (helper/inducer)-, OX8 (cytotoxic/suppressor)-, W3/13 (pan T lymphocytes)-, OX39 (IL-2)-, and OX41 (macrophages)-. These phenotypic markers were analogous to those of mice. The density of Thy-1+DC in the skin of normal nude F344 rats was approximately twice as much as that of F344 and WKAH rats. When WKAH rats were used as donors with nude F344 rats as recipients (this combination being a major mismatch with regard to a MHC, rejection-like changes such as edema and erythema were observed 1 week after the grafting. An immunohistochemical examination demonstrated that the density of Thy-1+DC had increased 3-fold within the donor skin at 1 week after transplantation, although almost no W3/25 positive cells, OX8 positive cells, or other inflammatory cells were observed. Within 2 weeks of transplantation the donor skin ceased rejection-like changes and the density of Thy-1+DC was reduced to preoperation levels. When WKAH rats were used as donors with F344 rats as recipients, severe rejection was observed, and many T cells with W3/25 or OX8 antigens and granulocytes had infiltrated into the skin at the donor sites. Thy-1+DC could not be found in the donor skin. These findings suggest that a correlation exists between Thy-1+DC and rejection-like changes in the donor skin when nude rats are used as recipients.
Animals↗
White pulp reconstitution after human bone marrow transplantation.
To reveal the reconstitution process of the white pulp after bone marrow transplantation (BMT), spleens of 24 marrow recipients whose survival times ranged from 34 to 303 days after BMT, were analyzed at the histopathological and immunohistochemical level. Up to 3 months after BMT, the white pulp was atrophic and consisted mainly of T cells forming periarteriolar lymphatic sheaths (PALS). Approximately 100 days after BMT, B cells aggregated in some of the white pulp, forming primary follicles, whereas marginal zones could not be detected. Beyond 4 months after BMT, the PALS, the lymphoid follicle, and the marginal zone of the white pulp could be seen in most of the recipients' spleens. However, the recovery of the marginal zone was poor up to 10 months after BMT. Thus, the white pulp was reconstituted sequentially, beginning in the PALS, followed by reconstitution in lymphoid follicles, and finally in the marginal zone. The development of the PALS corresponded well with the appearance of interdigitating dendritic cell, as did the development of lymphoid follicles with the appearance of follicular dendritic cell. The sequential reconstitution of the white pulp demonstrated in this study provides the morphological basis for the functional immune recovery of marrow recipients. In particular, the delay of the marginal zone reconstitution seems to be responsible for the functional asplenia of long-term survivors.
Adolescent↗
Ultrastructural and morphometrical studies on the reticular framework and reticular fibers in the reticuloendothelial system of rats.
The reticular framework and reticular fibers in the thymus, cervical lymph node, spleen and bone marrow of Wistar rats were studied by transmission electron microscopy and morphometrical method. The reticular framework was usually observed in these organs as a common structure that consisted mainly of the slender cytoplasmic processes of the fibroblastic reticular cells interconnected with tight junction. Ultrastructurally, the reticular fibers were a mixture of collagen fibrils and amorphous materials, and were almost completely ensheathed by the cytoplasm of fibroblastic reticular cells. Such characteristic structure of the reticular fibers was found not only in the thymus, lymph node and spleen, but also in the bone marrow where it has not been clearly demonstrated previously. Morphometrical analysis revealed that the content of collagen fibrils in the reticular fiber in the lymphoid tissues (the thymus, lymph node and splenic white pulp) was much greater than that in the hematopoietic tissues (the bone marrow and splenic red pulp). Based on these evidences, it was reasonably considered that the reticular framework and reticular fiber ensheathed by the cytoplasm of the fibroblastic reticular cells were the most representative common structure in the reticuloendothelial system (RES) and played some important roles in the functions of RES.
Animals↗
A murine model of experimental autoimmune lens-induced uveitis using Klebsiella O3 lipopolysaccharide as a potent immunological adjuvant.
Experimental autoimmune uveitis and finally panophthalmitis could be produced in mice by repeated immunization of syngeneic eyeball extract mixed with Klebsiella O3 lipopolysaccharide (KO3 LPS) as a powerful immunological adjuvant. No ocular lesions were produced in mice given eyeball extract emulsified in complete Freund's adjuvant (CFA), KO3 LPS alone or eyeball extract alone. Histopathological changes in the ocular lesions at the early stage after the second or tertiary immunization were characterized by infiltration with inflammatory cells in the ciliary body and iris. The iridocyclitis was followed by extensive infiltration of polymorphonuclear leucocytes (PMN) into the cornea, lens and the surrounding tissues after repeated immunization. Finally, these areas were replaced by granulomatous tissues infiltrated with mononuclear cells. On the other hand, the structure of the retina and sclera was partially preserved. Those mice exhibited production of autoantibodies and development of the delayed-type hypersensitivity (DTH) to syngeneic eyeball extract. Moreover, ocular lesions could be produced in normal recipient mice by transfer of sensitized lymphocytes from hyperimmunized mice. Therefore, it was suggested that the ocular lesions produced by repeated immunization with the mixture of eyeball extract and KO3 LPS were due to the autoimmune mechanism. This might be useful to model immunological phenomena in the pathogenesis of human phacoantigenic uveitis.
Adjuvants, Immunologic↗