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Biomedical subjects

J Albanese

Publications and source records attributed to J Albanese.

42 records · Page 3Linked to original sources
PubMed1986

The axillary vein: an alternative approach for percutaneous pulmonary artery catheterization.

The axillary vein route was investigated prospectively for percutaneous pulmonary artery catheterization in 79 patients who underwent 83 attempts. Forty nine of these patients were tracheostomized and under mechanical ventilation and eight had hemostasis disorders. Successful catheterization was achieved 74 times in the 79 patients. Pulmonary artery was reached within 6 +/- 2.1 min after the catheter was set in place in the axillary vein. Less than 1 min was needed in 53 cases. Puncture of the axillary artery was noted in 11 patients without complication. No other significant complication was noted following the punctures. Mean duration of catheterizations was 3.6 +/- 2 days. No septicemia was related to the catheterization procedure. One thrombosis of the axillary vein was noted. Catheterization of the pulmonary artery via the axillary vein is safe, simple and reliable and can represent an alternative method should the use of other routes be unsuccessful.

Adolescent↗
PubMed1982

Studies on the intercellular adhesion of rat and chicken hepatocytes. Tissue-specific adhesion in mixtures of hepatocytes and heart myocytes.

We previously reported that chicken and rat hepatocytes isolated from young adult animals displayed adhesive specificity in that they adhered preferentially to the homologous cell type. However, since we had used cells from two widely divergent species, it was not clear whether the cells were capable of distinguishing their own cell type from cells of other tissues of the same animal. The present experiments were aimed at determining whether, given the choice of adhering to cells obtained from another tissue from the same animal, cells still preferentially adhered to their own cell type, i.e. whether they showed tissue-specific adhesion. An improved collagenase perfusion procedure was developed for preparing single, viable heart myocytes. Cell adhesion experiments were then performed with hepatocytes and myocytes obtained from a single rat or chicken. Marked tissue-specific adhesion was observed under all conditions tested, which included varying the ratio of each cell type (hepatocytes or myocytes), stationary or gyratory conditions, the presence or absence of serum and certain metal ions, etc. The demonstration of tissue-specific adhesion among hepatocytes and myocytes isolated from the same animal is consistent with the idea that the two cell types contain different cell surface components required for cell-cell recognition. Furthermore, that the hepatocytes (and myocytes) can show tissue-specific adhesion validates the use of cells from adult animals for biochemical studies on intercellular adhesion.

Animals↗
PubMedDate not supplied

Use of a rat cDNA probe specific for the Y chromosome to detect male-derived cells.

A cDNA probe that exhibits specificity for the rat Y chromosome was generated by using a set of primers specific to the murine Sry gene, the sex-determining region of the Y chromosome. A 459-base pair (bp) DNA fragment was obtained by polymerase chain reaction (PCR) amplification from male, but not female, rat genomic DNA (EMBL Nucleotide Sequence Database accession number X89730). This DNA fragment was purified, cloned using a vector, and digested with EcoR1 to yield a 270-bp DNA fragment. This 270-bp cDNA fragment, when used as a probe in Southern blot analysis of rat DNA, was observed to bind to three separate bands of approximately 2.3, 5.0, and 7.0 kb in size. The binding was demonstrated with male, but not female, genomic DNA. Another set of primers was generated to sequences within the 270-bp fragment that produced a PCR product of 104 bp. This DNA fragment, when used as a probe in Southern blot analysis, enabled PCR detection of at least 0.1% male cells in a mixed population of female cells. These cDNA probes should prove useful in studies designed to track cell populations (e.g., tumor metastasis and hemopoietic cells after bone marrow transplantation) in syngeneic male/female pairs. In addition, a cDNA probe that is specific for the rat Sry gene might be valuable in studies of fetal male sexual development or the study of spermiogenesis.

Animals↗