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J A Serrano

Publications and source records attributed to J A Serrano.

35 records · Page 2Linked to original sources
PubMed1980

Particle displacement in epithelial cell membranes of rat prostate and pancreas induced by routine low temperature fixation.

When carried out at 4 degrees C in contrast to 37 degrees C, glutaraldehyde fixation for routine freeze-fracture specimen preparation results in displacement of membrane particles of epithelial cells of rat ventral prostate. Particle-free areas are observed in endoplasmic reticulum (ER), nuclear envelope, and Golgi membranes; particle aggregation is observed in lateral and basal portions of the plasma membrane. Multilamellar arrangement of lipids can be observed in some of the large particle-free areas. Thin section observation indicates that in the ER, particle-free areas coexist with segregation of cytoplasmic ground substance from the membrane surfaces. Particle-free patches are also observed in the ER membranes of pancreatic tissues fixed at 4 degrees C.

Animals↗
PubMed1979

The ultrastructural localization of secretory acid phosphatase in giant cell tumor of bone.

Three cases of giant cell tumor of bone were studied with the light and electron microscopes to determine the histochemical and cytochemical distribution of acid phosphatase isoenzymes. Using beta-glycerophosphate as a nonspecific substrate, acid phosphatase was found in the giant cells as well as the stromal cells. Ultrastructurally, using this substrate, the enzyme was found to be associated with lysosomes in the stromal cells and giant cells and also with the profuse system of tubules and vesicles in the cytoplasm of the giant cells. Using phosphorylcholine and D-ephedrinephosphate, which are substrates for a specific secretory isoenzyme of acid phosphatase, activity was found only in the vesicles of the giant cell cytoplasm. Lysosomes did not show activity with these substrates. Multinucleated cells of giant cell tumor contain a specific secretory type of acid phosphatase which is not present in the stromal cells. This suggests that the giant cells are attempting to form a secretory system similar to osteoclasts and that the vesicles in the cytoplasm which contain this acid phosphates are the morphologic expression of an abortive secretory system.

Acid Phosphatase↗
PubMed1978

D-Ephedrinephosphate, DEP: a new substrate with specificity for prostatic acid phosphatase (PAP).

This report describes the synthesis and physical (including spectral) properties of a new substrate, D-ephedrinephosphate, DEP1, which is histochemically highly specific for a secreted non-lysosomal prostatic acid phosphatase, PAP. This specificity is in contrast to other substrates which are nonspecific, i.e., which demonstrate acid phosphatases that originate from various cell types and are mainly lysosomal. When this substrate is used for light and electron microscopic histochemistry in a modified Gomori medium, PAP is demonstrated mainly in secretory granules and in the Golgi apparatus (and its related vacuoles) of prostatic epithelial cells of several species of mammals including man. This corroborates our previous suggestion that PAP is not a lysosomal enzyme as are many of the other acid phosphatases. This high degree of specificity of DEP for PAP supports the usefulness of this compound in the histochemical and biochemical characterization of PAP, and in the diagnosis of localized or disseminated prostatic disease.

Acid Phosphatase↗
PubMed1978

Hemodynamics and coronary angiography in idiopathic hypertrophic subaortic stenosis.

One hundred eighteen patients with idiopathic hypertrophic subaortic stenosis were studied with cardiac catheterization and coronary arteriography. In 112 a gradient across the left ventricular outflow tract was present in the resting state. Seventeen of the 61 patients who had right heart catheterization had a mild resting gradient across the right ventricular outflow tract, that was considered clinically and hemodynamically insignificant. Ninety-five patients (80 percent) had a left ventricular end-diastolic pressure greater than 10 mm Hg; 60 percent had mitral regurgitation that was of mild degree in most cases. Almost 20 percent had coexistent coronary atherosclerosis (25 percent incidence rate in subjects aged 40 years or older). Patients with associated severe coronary atherosclerosis had a lower intraventricular gradient at rest than other patients. Coronary atherosclerosis appears to be a coincidental condition. The need for objective evaluation of the coronary circulation is emphasized.

Adult↗
PubMed1977

The histochemical demonstration of human prostatic acid phosphatase with phosphorylcholine.

Tissues from patients with benign prostatic hyperplasia were used for the cytochemical demonstration in light and electron microscopy of a secreted, nonlysosomal prostatic acid phosphatase (PAP) with phosphorycholine, substrate specific for PAP. The specificity of phosphorylcholine for PAP is attributable to the pentavalent nitrogen in phosphorylcholine, a feature that renders it resistant to hydrolysis by all other acid phosphatases. PAP activity was found in the Golgi cisternae and its associated vacuoles and in secretory vacuoles localized in the nuclear, Golgi, and apical areas of the prostatic epithelial cell. These results confirm the existence of two types of acid phosphatase in prostatic tissue. One is lysosomal and is prevalent in many tissues and the other, PAP, is the major enzymatic product secreted by the prostate. The specificity of PAP for phosphorylcholine, one of the natural substrates for this enzyme, validates the use of this method for the histochemical characterization of PAP and indicates the prostatic origin of cells showing PAP activity.

Acid Phosphatase↗
PubMed1976

The cytochemical demonstration of prostatic acid phosphatase using a new substrate, phosphorylcholine.

Prostatic acid phosphatase (PAP), an acid phosphatase specific to the prostate gland, is demonstrated cytochemically for both light and electron microscopy with a new substrate phosphorylcholine. Lead ion is used as capture agent for liberated phosphate ion in a modified Gomori medium. PAP is demonstrated in the tubuloaveolar epithelial secretory cells of the rat ventral prostate gland. In the apical portion of the cell it is found in secretory granules and in the matrix of multivescular bodies. In the Golgi area it is localized in Golgi cisternae, Golgi related vacuoles and multivescular bodies. Evidence is presented that PAP is not a lysosomal enzyme, as are other acid phosphatases, and that phosphorylcholine is a highly specific substrate for PAP. As based on the role of pentavalent nitrogen on substrate structure, it is apparent that PAP is to other acid phosphatases what the cholinesterases are to other esterases.

Acid Phosphatase↗
PubMedDate not supplied

[Phenomenology of exile: the exiled person and his family].

The experience of exile is the result of the interaction between the protagonists of this human tragedy and the society of refuge. The ambivalence of the refugee and his family is due to the loss of the original narcissistic supports and the need to assert the new situation. The article explains the characteristics of the experience of exile, at the individual and family level, and underlines the double challenge which has to be accepted by the exile and his family as well as by the society of refuge.

Acculturation↗
PubMedDate not supplied

New agents for prostatic cancer activated specifically by prostatic acid phosphatase.

Potential cytotoxic compounds (spindle poisons) are being designed for the treatment of prostatic carcinoma, using the structural requirements of the substrates for prostatic acid phosphatase (PAP). Colchicine has been modified in ring C to give colchiceinamides of substituted ethanolamines and ethanolaminephosphates. Another new series of compounds modifying ring B of thiocolchicine have been prepared. Three O-phosphates of the thiocolchicine series have also been made. One has been examined for its specificity toward PAP. Some toxicity data of these compounds in mice have also been reported. Colchiceinamide-(L)-ephedrinephosphate has been examined in stumptail monkeys and some preliminary results are reported here.

Acid Phosphatase↗
PubMedDate not supplied

[Space, time and identity in migrant (author's transl)].

An understanding of the psychology and psychopathology of the migrant is possible from the phenomenological description of his living world, by utilizing categories of space, time and identity. The clinical preponderance of depressive, paranoïd and psychosomatic symptoms and the symptomatic polymorphism are integrated in this perspective. This includes the subject and the social structure as elements of the total picture. The constant relationship of the subject with the social structure can point to the sociogenic reactive character of some troubles (Acta psychiat. belg., 1977, 77, 623-638).

Belgium↗