Enolase isoenzymes as diagnostic markers.
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APUD Cells↗
Biomedical subjects
J A Royds
Publications and source records attributed to J A Royds.
Monoclonal antibody to aldolase C: a selective marker for Purkinje cells in the human cerebellum.
A monoclonal antibody to the glycolytic enzyme aldolase C was used in an immunoperoxidase technique on unfixed and formalin-fixed paraffin-embedded tissues from the human central nervous system (CNS) and other tissues. No staining outside the CNS was detected. In the central nervous system the antibody gave a selective and intense staining of Purkinje cell perikarya, axons and dendrites in both fixed and unfixed tissues. In the human fetal brain, positive staining of Purkinje cells was seen from 35-36 weeks' gestation onwards. This coincides with the conversion of Purkinje cells to a tetraploid state, and may reflect increased glycolytic activity accompanying the rapid dendritic growth at this stage of development. Preliminary investigations on a variety of disease states suggest that this antibody may be useful in studies of ischaemic damage, congenital abnormalities and degenerative conditions affecting the cerebellum.
Adult↗
Enolase isoenzymes in benign and malignant melanocytic lesions.
The distributions of the alpha, beta and gamma subunits of enolase were studied in 34 melanocytic lesions using the peroxidase-antiperoxidase (PAP) technique. Melanocytes in benign neoplasms stained moderately strongly for both alpha enolase and gamma enolase but staining for beta enolase was consistently negative. Staining for alpha enolase was always stronger than that for gamma enolase. There are some indications that with increasing degrees of dedifferentiation there was a reduction of gamma enolase activity, least differentiated cells being completely negative. Ocular melanomata showed similar changes to those seen in the skin. The enolase isoenzyme composition of tumour extracts was assessed biochemically on a limited number of cases and these results are discussed in relationship to the immunohistochemical studies.
Humans↗