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Biomedical subjects

J A Molinari

Publications and source records attributed to J A Molinari.

At least 91 records · Page 5Linked to original sources
PubMed1987

Stability of safety glasses during sterilization and disinfection.

Awareness of the necessity for physical barriers as essential components of an infection control program for dental practice has led to increased use of gloves, masks, and protective eyewear. Because eyewear is not disposable, it could serve as a source of cross-contamination and should be disinfected between patients. This study investigates the optical stability of two types of safety glasses after various sterilization/disinfection procedures.

Communicable Disease Control↗
PubMed1986

Comparative efficacy of ketoconazole and mebendazole in experimental trichinosis.

The therapeutic efficacy of ketoconazole and mebendazole was studied in ICR/CD-1 mice infected with Trichinella spiralis for 17 to 20 weeks. Efficacy of both drugs was over 70% when compared with results in control mice. This study indicates that both ketoconazole and mebendazole should be considered in the treatment of trichinosis in humans.

Animals↗
PubMed1985

Oral candidiasis: forerunner of acquired immunodeficiency syndrome (AIDS)?

A 42-year-old man who used intravenous drugs had oral candidiasis and weight loss. Careful examination led to a diagnosis of AIDS. The patient subsequently died of disseminated cryptococcosis, systemic candidiasis, and polymicrobial bacteremia. The circumstances and progression noted in this case led to the recommendation that an aggressive screening for AIDS should be performed on patients at high risk for this syndrome who present with oral Candida infection.

Acquired Immunodeficiency Syndrome↗
PubMed1983

Herpetic whitlow: report of a case with multiple recurrences.

A case of recurrent herpetic whitlow is discussed. The patient, a dental assistant at the time of her initial infection, developed herpetic whitlow caused by herpes simplex virus type 2 (HSV-2). She has subsequently experienced nine recurrent infections with lesion spread, scarring, and persistent paresthesia. The severity of the most recent recurrences appears to have diminished, and prodromal sensations, that is, increased local sensitivity, have occurred without the subsequent development of lesions.

Adolescent↗
PubMed1982

Two cases of incompatibility to carbon-coated subperiosteal implants.

Two cases of clinical incompatibility of carbon-coated subperiosteal implants are presented. The clinical history and the microscopic features of biopsy material of each case are reviewed. The histologic appearance of tissue from both cases presented scattered particles of carbon surrounded by lymphocytes, macrophages, plasma cells, and tissue eosinophils. Active phagocytosis and Russell's bodies were seen. Large masses of carbon were surrounded by connective tissue. The clinical and microscopic findings suggest an immunologic rejection of the implant-coating material.

Biocompatible Materials↗
PubMed1982

Potentiation of MIF production by lymphocytes of Trichinella spiralis-infected mice.

In order to further investigate the mechanism involved in the immunopotentiation following Trichinella spiralis infection, mice were injected intraperitoneally with either heat-killed or viable bacillus Calmette-Guérin (BCG) 28 days after oral infection with 200 nematode larvae. Delayed-type hypersensitivity (DTH) was assessed 28 days later by measuring levels of migration inhibitor factor (MIF) elicited by Old Tuberculin challenge. Sensitization with killed BCG produced only negligible amounts of MIF; however, prior T. spiralis infection resulted in significantly increased titers of MIF, reaching levels induced by viable BCG. The data indicated that a lymphokine component may be involved in addition to non-specific mechanisms previously proposed.

Animals↗
PubMed1982

Sequential mesenteric lymph node histological response following Trichinella spiralis infection.

The histology of mouse secondary lymphoid tissue was examined following infection of Trichinella spiralis. ICR/CD-1 mice were orally infected with 200 T. spiralis and sacrificed at either 0, 3, 7, 14, 28 or 56 days after nematode administration. The animals were bled and sacrificed, and mesenteric lymph nodes were removed from each animal. They were fixed in 10% neutral buffered formalin, embedded in paraffin, and cut in 4-micron sections. Examination of serial sections demonstrated the sequential development of histological alteration in these regional lymph nodes. Hyperplasia was detected as early as 3 days after infection. Progressive changes occurring between 7 and 28 days after larval inoculation included hyperplasia with concurrent congestion of medullary sinuses, with increased numbers of neutrophils, histiocytes, lymphocytes, and eosinophils. Microscopic appearance of nodes removed from 56-day infected mice was similar to that noted for control tissues.

Animals↗
PubMed1981

Dose dependence of Trichinella spiralis-induced immunopotentiation.

Previous reports studying the immunological and histopathological sequelae following infection with Trichinella spiralis indicated that this nematode can affect the host's immune capability to heterologous antigens. The present investigation was aimed at determining the dose of T. spiralis larvae required for development of maximum delayed-type hypersensitivity (DTH) against heat-killed bacillus Calmette-Guérin (BCG). Mice were injected intraperitoneally with 4 x 10(6) bacteria 28 days after oral intubation with 0, 50, 100, 200, 300 or 400 viable T. spiralis larvae. Groups were tested in vivo for DTH 28 days after administration of nonviable BCG by the footpad swelling assay. Prior infection with 200 nematode larvae resulted in 34 of 42 animals (81%) developing positive 24-hour DTH reactions. When these data were compared to those seen in the other experimental and control groups, the 200 larval dose appeared to effect maximal immunopotentiation in this system.

Animals↗
PubMed1979

Influence of Trichinella spiralis infection on development of sarcoma-180 ascites tumors.

Long-term infection of mice with Trichinella spiralis has been shown to stimuate increased host protection aginst transplantable solid tumors. The present investionation was initiated to determine whether parasitized mice were similarly protected from induction and progression of an ascites tumor. ICR/CD-1 mice were orally infected with 50, 100, 200, 300, or 400 T. spiralis larvae, and subsequently challenged intraperitoneally with 5 X 10(4) Sarcoma-180 (S-180) ascites cells. Animals were observed and weighed daily for development and progression of malignancy. Protection from fatal ascites neoplasia was found to be statistically significant under selected conditions of larval dose and challenge interval. Mice infected with 100 or 200 larvae were more resistant to S-180 progression than both uninfected controls or other infected groups. Protection was observed in groups challenged 2 weeks after nematode intubation but not at 6, 8 or 34 weeks. This finding is in contrast with long-term protection seen previously with B-16 melanoma tumors, and suggests that antineoplastic effects of T. spiralis infection differ for ascitic and solid murine tumors.

Animals↗
PubMed1978

Effect of Trichinella spiralis infection on delayed hypersensitivity to heterologous antigens.

Studies were initiated to determine the effects of the different phases of the nematode life cycle on the delayed-type hypersensitivity (DTH) capabilities of the host. Mice were injected intraperitoneally with 4 X 10(6) viable or heat-killed BCG at 0, 3, 7, 14 and 28 days after oral infection with 200 Trichinella spiralis larvae. Groups were tested for DTH 28 days after BCG inoculation by the footpad swelling assay. Infection with T. spiralis was found to suppress the response in mice administered viable BCG 0 or 3 days later. Mice injected with viable bacteria 7 days after T. spiralis regained the ability to develop in vivo DTH reactions against old tuberculin. Groups administered viable BCG 14 and 28 days after nematode infection yielded potentiated DTH responses, when compared with control mice. Animals infected with T. spiralis and later injected with heat-killed BCG required a longer interval to develop specific immunopotentiation. Maximum immunopotentiation seemed to be related to the presence of larvae in muscle tissue (28 days). These findings suggest that the effect of T. spiralis on the immune capabilities of the host is dependent on the different anatomical locations of the parasite.

Animals↗