Microbial disease trends and acquired antibiotic resistance: Part 2: Evolving microbial pathogens--antibiotic-resistant Staphylococcus aureus.
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Biomedical subjects
Publications and source records attributed to J A Molinari.
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A 23-year-old woman was diagnosed with a 5-cm interstitial pregnancy by transvaginal ultrasound. In addition, she was positive for the human immunodeficiency virus. She underwent laparoscopic cornual excision of the ectopic gestation and was discharged home the next day.
Hepatitis C, previously termed parenterally transmitted NANB hepatitis, is caused by a single-stranded RNA virus (HCV). Infection with this virus has epidemiologic characteristics similar to hepatitis B, with infection from contaminated blood appearing to be a primary source of disease. Although 80% to 90% of the viral hepatitis associated with blood transfusions used to be the most common mechanism for HCV transmission, parenteral drug abuse has become the major documented risk factor in the United States. Health care workers face occupational hazards for HCV infection primarily through needlestick and other contaminated sharps accidents, although the risks are much lower than those historically documented for HBV. Current universal bloodborne precautions for infection control appear to be effective against occupational cross-infection in patient care settings. Ongoing studies in patients with long-term persistent hepatitis C are investigating the usefulness of prolonged interferon therapy to reduce hepatic damage and HCV chronicity.
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Microbial adherence to the internal surface of dental tubing and the formation of a highly protective biofilm layer is predictable, given the ideal growth conditions in the tubing. The slime layer is the most common cause of dental water contamination. Various environmental and human-derived potential pathogens have been reported worldwide. Dental equipment such as retracting shut-off valves, antiretracting valves that tend to fail, or waterlines that are inaccessible contribute to a situation in which virtually every standard dental unit contains contaminated water. While exposure to Pseudomonas, Moraxella, Staphylococcus, and Legionella has been linked to dental water, the medical risk of dental unit waterline contamination is most significant to immune-deficient individuals. Regulations and technological devices are emerging to manage dental water quality.
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Eleven patients with 36 stable endosseous implants in function for at least six months were selected for culturing in this study. Each patient had been restored with a mandibular implant-borne bar overdenture. The bacterial samples were taken from the superior aspect of the implants after bar and abutment removal. The plaque specimens contained predominantly Gram-positive microflora, although Gram-negative species were present in low numbers. This study serves to corroborate previously published reports regarding the characterization of the microflora associated with stable endosseous implants.
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Optimal infection control is achieved more by understanding infectious diseases than by meeting federal and state regulations. A basic review of microbial transmission, aseptic technique and universal precautions provides a better understanding of the rationale behind recommended procedures.
Sterilization in the dental office should be monitored to ascertain proper sterilizer function. Biologic monitoring with calibrated preparations of bacterial spores is the preferred, as well as the only method that actually measures sterilization. A dental school-based sterilization monitoring service for dental practices was established in 1978 at the University of Detroit. This service has grown from 20 participating dental offices to more than 1,500. In 1993, 18,137 biologic monitoring tests were performed. The participants in the service primarily use autoclaves (70%) for heat sterilization, while unsaturated chemical-vapor sterilizers (20%) and dry-heat units (10%) are less common. This article describes the history of sterilization monitoring in dental practices from 1978 to the present through a dental school-based service.