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Biomedical subjects

J A Madej

Publications and source records attributed to J A Madej.

At least 19 recordsLinked to original sources
PubMed1998

Therapeutic potential of biological response modifiers against transplantable mouse tumors of spontaneous origin. II. Local interleukin 2 treatment of tumors of different immunogenic strength.

This report describes tumor susceptibility to interleukin 2 (IL-2) therapy in relation to tumor immunogenicity. The following lines recently established from spontaneous tumors were used: X5, X6, and X9 mammary tumors of DBA/2, BALB/c and CBA origin, respectively, X7 carcinoma of BALB/c and X18 papilloma of CBA mice. Two spontaneous tumors of long transplantation history, SL2 lymphoma (SL2) of DBA/2 and M109 Madison lung carcinoma (M109) of BALB/c origin, were used as control systems. Mice were transplanted with different inocula of tumor cells at day 0; IL-2 treatment was initiated on day 1-3 or 10 and consisted of daily injections of 5000 or 20,000 IU/mouse given 5 times a week for 3 weeks. SL2 (i.p. - 2 x 10(4) cells) treatment consisted of i.p. injections of 5000 or 20,000 IU IL-2 given on days 10-14. IL-2 therapy of SL2 bearing DBA/2JIco mice resulted in the significant proportion of cures; however, no response to IL-2 treatment was achieved in SL2 bearing DBA/2Crliw mice. BALB/c mice with the i.p. transplant of M109 responded to IL-2 treatment with 40% increase in lifespan. The low-dose IL-2 therapy of the 5 recently established tumors resulted, in general, in transient growth inhibition of the i.m. transplants of line X5, X6, and X7 provided IL-2 was administered locally. The therapeutic effect depended on the number of transplanted tumor cells, and the best results being achieved at cell numbers close to the dose inducing tumor growth in 50% of animals (TD50). We found that the tumors responding to IL-2 treatment were all slowly growing and immunogenic (X6 and X7) or might have viral association (X5) and as such might express foreign antigens.

Adjuvants, Immunologic↗
PubMed1995

Ultrastructural studies on organs of cadmium-poisoned rats treated with oxygen-ozone mixture.

Rats poisoned with cadmium acetate during 12 weeks, at a dose of 50 mg/dm3 given in drinking water, were treated with oxygen-ozone mixture as intraperitoneal injection during the last 10 days of the experiment, at a daily dose of 1 cm3 and ozone concentration 40 micrograms/cm3. The mixture was made of medical oxygen with a Bioozon U type apparatus produced by B. Prochazka GmbH, Germany, Reutlingen. Control groups included animals treated with the above mixture with no cadmium, and rats poisoned with cadmium, with no oxygen-ozone treatment. Liver and cardiac muscle were examined in TEM Philips EM 301. Morphological traits of a protective of the mixture against cadmium-poisoning were observed in both those organs. This was expressed as weaker destructive changes within the endoplasmic reticulum, basal cytoplasm and lysosome of the hepatocytes, and additionally as a stabilization of contractile apparatus fibres in the heart myocytes.

Administration, Oral↗
PubMed1993

Further studies on the etiopathology of malabsorption syndrome in broiler chickens.

The paper presents two cases of malabsorption syndrome in broiler chickens. The results indicate that the etiological factor appears to be the virus agent which causes necrosis in extra-excretional parts of the pancreas. During the course of the disease disorders in the enzymatic activity of gammaglutamyltransferase, leucylaminopeptidase and alanylaminopeptidase were observed. It consequently leads to the deterioration of intestines digestion and resorption of feed components. Application of Selcewet-Polfa to the diseased birds improved the health of the birds, morphological picture of peripheral blood and mineralisation of thigh bones. No radical improvement in the compensation of the final body weight was recorded.

Animals↗
PubMed1993

The influence of long-term administration of Tołpa Peat Preparation on immune reactivity in mice. I. Morphological changes in the thymus.

In experiment I BALB/c 200 mice were given in drinking water the TPP (Tołpa Peat Preparation) in daily doses of 1, 10 and 50 mg/kg over the period of 3, 5, 7, 9 and 12 weeks. In experiment II before the administration of TPP (as in experiment I) mice were immunized with a single dose i.p. administration of 0.2 cm3 of a 10% suspension of sheep erythrocytes (SRBC), i.e. 4 x 10(8) cells. Histopathological and ultrastructural studies have shown that TPP in all three doses causes morphological changes characteristic of thymus activity stimulation. However, the doses of 10 and 50 mg/kg administered longer than 7 weeks caused retrograde changes that did not occur after administration of 1 mg/kg of TPP. Morphological changes in the thymus of immunized and non-immunized mice simultaneously receiving TPP were similar.

Adjuvants, Immunologic↗
PubMed1993

The influence of long-term administration of Tołpa Peat Preparation on immune reactivity in mice. II. The effect of intermittent TPP administration on the morphological picture of lymphatic organs.

The studies were conducted on 200 Balb/c mice (inbred strain), aged 8 weeks. The mice were administered Tołpa Peat Preparation (TPP) in drinking water (10 or 50 mg/kg/day) for seven weeks (experiment I). Two lobes of the thymus, the spleen and the axillary and mesenteric lymph nodes of mice were taken for histological examinations after 3, 5 and 7 weeks of continuous TPP treatment and also 12 and 16 weeks from the beginning of the treatment. In experiment II, TPP was administered daily at the same doses for 3 weeks, and intermittently for 9 consecutive weeks; a week interval followed each seven-day cycle. The lymphatic organs of mice were taken for histological examinations after 3, 5, 7, 9 and 12 weeks of TPP treatment. It was found that daily administration of TPP (as described above) to mice for 7 weeks (10 mg/kg) induced a marked stimulation of the lymphatic organs (in the first weeks of TPP treatment) and resulted in retrograde changes (depletion of thymocytes and lymphocytes) in the lymphatic structures when TPP administration was prolonged (4-7 weeks). The morphological changes due to TPP administered at the dose of 50 mg/kg were less pronounced in the organs examined. The retrograde changes in the lymphatic structures disappeared 9 weeks after TPP treatment had been stopped. Similarly, the changes showing stimulation of the lymphatic organs were maintained throughout the entire period of 9 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)

Adjuvants, Immunologic↗
PubMed1993

Histological and ultrastructural examination of muscles in broilers administered tiamulin together with ionophoric anticoccidials.

Histopathological and ultrastructural examinations of muscles in broilers, receiving tiamulin and ionophoric anticoccidials were carried out. Myo- and cardiomyopathies, originating from primary mitochondria lesions were followed by ATP deficiency, oedema, degeneration and necrosis of myocytes. This process is reversible and manifested by slow regeneration of muscles.

Animals↗
PubMed1993

Therapeutic potential of biological response modifiers against transplantable mouse tumors of spontaneous origin. I. Characterization of biological properties of tumor lines and preliminary data on response to cytostatic drugs and biomodulators.

Several transplantable lines were derived from spontaneous tumors of mouse strains of low and high cancer incidence (CBA, BALB/c and DBA/2), and eight of them were used for an evaluation of the therapeutic potential of BRM. The lowest number of cells inducing tumors was in most cases around 10(4) cells. In transplantation tests, tumors of CBA and DBA/2 mice were non-immunogenic, while tumors of BALB/c mice showed different level of immunogenicity. Transplantation of a small number of tumor cells in admixture with BCG resulted in no tumor growth of the immunogenic line X6 and no effect on take rate of non-immunogenic line X1. Sensitivity of studied tumor lines to BCNU, CY and ADR was moderate. Therapy of the tumors with postulan and CMA, two biomodulators already shown to be active against various experimental tumors, failed. The significant therapeutic response was observed in DBA/2 mice bearing i.p. transplant of lymphosarcoma X19 and treated with i.p. injections of CMA.

Animals↗
PubMed1992

Amino acids and cholesterol levels of leukemic lymphocytes infected with bovine leukemia virus (BLV).

We performed qualitative and quantitative amino acids analysis of lymphocytes from normal and leukemic cattle (infected with bovine leukemia virus) to determine cellular cholesterol levels. Leukemic lymphocytes exhibited quantitative differences in the levels of some "glycogenic", "ketogenic" and "glyco-ketogenic" amino acids. These differences may reflect the disturbances of Krebs cycle. Evidence was produced that increased cholesterol content in the above lymphocytes was associated with disorders in beta-oxidation processes of leukemic cells.

Amino Acids↗
PubMed1991

[The content of Ca2+ and Mg2+ ions and membrane enzyme activity (AP, 5'-NT, ATPases) in the lymphocytes infected in vitro with bovine leukemia virus].

In the lymphocytes infected in vitro with BLV (bovine leukemia virus) the contents of Ca2+ and Mg2+ were determined using roentgen microanalyser JXA-5 A Joel-form (Japan). In the smears prepared from these cells the activity of enzyme markers of cell membranes i.e. alkaline phosphatase (AP - EC 3.1.3.1), 5'-nucleotidase (5'-NT - EC 3.1.3.5) and adenosine-triphosphatases - Ca2+ and Mg2+ dependent (ATP-ase - EC 3.6.1.3) was determined. The decrease in AP and ATP-assess activity and increase in 5'-NT in the membranes of leukemic lymphocytes were observed. During these changes the increase in Ca2+ and decrease in Mg2+ ions occurred. These processes lead to clear disturbances in the metabolism of cells transformed by the neoplasm. The effect of this phenomenon is probably the opening of calcium canals with the following cytoplasmatic hypercalcemia. It's very destructive for the change in permeability of the membrane of lymphocytes.

5'-Nucleotidase↗
PubMed1989

In vitro studies into some parameters of protein and carbohydrate metabolism in lymphocytes infected with bovine leucosis virus.

Several parameters of protein and carbohydrate metabolism were determined in normal and leukemic lymphocytes in vitro in cattle, including arylamidase activity toward beta-naphthylamides of L-amino acids. The homogenate of bovine leukemic lymphocytes, in comparison with the control revealed increase of gamma-glutamyltransferase, activity trypsin inhibitor and papain inhibitor concentration and aldolase activity. On the other hand, proteolytic activity toward casein and histomucoid content decreased. Out of the 7 substrates used in the study, only 2, alanyl-beta-naphthylamide and leucyl-beta-naphthylamide, demonstrated lower activity in the leukemic material. Disorders in carbohydrate and protein metabolism in the observed lymphocytes in vitro in cattle are presented in the paper.

Animals↗
PubMed1989

The content of fatty acids in leukemic lymphocytes in vitro in cattle.

In leukemic lymphocytes in vitro obtained from cattle the fatty acids content has been determined employing the method of gas chromatography of methyl esters. Significant increase of linolic acid (C18 = 2) and of linolenic acid (C18 = 3) in leukemic lymphocytes have been noted, while reduced amounts of eicanoic acid (C20) and arachidonic acid (C20 = 4) have been observed. Fatty acids contribution in destabilization of biological membranes of leukemic cells has been discussed. It is very likely that the destabilization of biological membranes promotes the "induction" of various cancero- and cocancerogenetic changes, as well as enables the penetration of leukemovirus into the cell.

Animals↗
PubMed1988

[Superoxide dismutase, catalase and glutathione peroxidase activities in mice with natural lymphocytic leukemia].

In the mice of the high leukemic strain, sick with natural lymphatic leukemia, the following parameters were determined on the 0 day, after 90, 180 and 270 days of observation: activity of the superoxide dismutase (SOD-EC1.15.1.1), glutathione peroxidase (GSH-px-EC1.11.1.9) in the blood and catalase activity in erythrocytes. It was observed that the activity of all three enzymes during the development of the leukemic process decreased. This phenomenon indicates the disturbance of the protective role of these enzymes against free radicals in leucogenesis in mice.

Animals↗
PubMed1988

[Levels of copper and its antagonists in mice with natural lymphocytic leukemia].

In the mice of high leukemic strain, sick with natural lymphatic leukemia, levels of copper, zinc and cadmium in blood and inner organs were determined by the method of atomic absorption spectrophotometry. Mice were killed on the 0 day (when 10 weeks old) and after 90, 180 and 270 days of observation. In plasma the level of ceruloplasmin (EC1.12.3.1) was determined. It has been proved that in mice with lymphatic leukemia the levels of copper, zinc and cadmium are higher than in control animals. It was also found out that there is some disturbance in the natural antagonism between these metals. The activity of ceruloplasmin in the course of leukemia was determined. We have also tried to interpret the role of heavy metals in leukemogenesis in mice.

Animals↗
PubMed1988

[Effect of copper and selenium on the histopathologic picture of the internal organs and the accumulation of these metals in hens].

The hens were given per os every second day for the period of 6 weeks selenium in the amount of 0.2 mg/l water (group I), 1.4 mg (group II), copper in amount of 1000 mg/kg feed (group III) and both metals jointly (group IV)--0.2 mg Se and 1000 mg Cu and group V--1.4 mg Se and 1000 mg Cu. Histopathological studies on hens organs and accumulation of metals in tissue were carried out. It was found that selenium in the amount of 1.4 mg/l water causes insignificant oedema of liver and kidneys, whereas copper (group III) shows clear hepato- and nephropathic effect. Selenium administered jointly with copper (group V) acts protectively in parenchymatous organs against the toxic effect of copper alone.

Animals↗
PubMed1988

Serum cobalt-activated acylase as a marker of transplantable leukemia in mice.

The activity of cobalt-activated acylase was determined in the serum of mice with transplantable leukemia (P 388, L 1210 standard, L 1210/ara-C, L 1210/CH3-G, plasmocytoma ADJPC-5, lymphoma AKSL-4 and natural leukemia in mice NZB). A statistically significant increase in enzyme activity in all leukemias except lymphatic leukemia has been demonstrated. The results suggest possibility of using the enzymatic measurement as a marker of transplantable leukemia in mice.

Aminopeptidases↗
PubMed1987

Activity of some hydrolases in mice with transplantable lymphatic leukemia P 388.

Mice--BDF, hybrides, males aged 3 months were injected intraperitoneally with 10(4) lymphatic leukemia cells P 388. The mice were killed by bleeding after 2.5 and 10 days after the injection. Gamma-glutamyltransferase (GGT), leucylaminopeptidase (LAP) and cobalt activated acylase activity were determined in the serum of the mice. The inner organs of the animals were verified histopathologically. A statistically significant increase in the activity of the examined enzymes was observed together with the development of transplantable leukemia. These enzymes can be helpful in early monitoring of lymphatic leukemia P 388 in mice--as markers of cancer growth.

Amidohydrolases↗