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J A Kane

Publications and source records attributed to J A Kane.

30 records · Page 2Linked to original sources
PubMed1973

Filamentous capsulated streptococci from the human respiratory tract. I. Antigenic attributes of provisional capsular type 83 and its relationship to streptococci of so-called group M.

Two immunologically reactive polysaccharides have been isolated from the cell walls and from culture filtrates of a filamentous alpha-hemolytic streptococcus provisionally designated capsular type 83. Both polysaccharides were purified by diethylaminoethyl-cellulose chromatography. Analysis indicates that the capsular polysaccharide consists of galactose and phosphorus, whereas the cell wall polysaccharide contains galactosamine, glucosamine, glucose, and phosphorus. On the basis of immunochemical experiments, it is suggested that the capsular polysaccharide is composed of galactose-phosphate units with terminal galactose residues at the nonreducing end. It has also been found that the capsular antigen of streptococcus type 83 is shared by a number of streptococcal strains classified in Lancefield's group M. The cell wall polysaccharide of streptococcus type 83 cross-reacts with antibody to the C(s), or cell wall-like capsular, polysaccharide of Diplococcus pneumoniae, and this cross-reactivity may be a reflection that the streptococcal antigen possesses certain structural features which are similar to those of pneumococcal C and C(s) polysaccharides.

Antigens, Bacterial↗
PubMed1973

Filamentous capsulated streptococci from the human respiratory tract. II. Antigenic structure of provisional capsular types 89 and 83-89.

Two immunologically distinct polysaccharides have been isolated from the filamentous alpha-hemolytic streptococcus of provisional capsular type 89, recovered from the human respiratory tract. Chemical analyses indicate that the capsular polysaccharide consists of glucose, galactose, and a small amount of rhamnose, whereas the cell wall-associated polymer contains galactosamine, glucosamine, glucose, and phosphorus. Immunological studies suggest that the capsular polysaccharide is type specific and that the cell wall-associated carbohydrate, which cross-reacts with the C(8), or cell wall-like capsular polysaccharide of pneumococcus, may be group specific. A noncapsulated variant of the prototypic streptococcus of provisional type 89 was shown to possess the same cell wall-associated carbohydrate as the strain from which it was derived, but it proved to be poorly antigenic in rabbits. A filamentous capsulated streptococcus reacting with antisera to filamentous streptococci of both provisional capsular types 83 and 89 has been found to produce two capsular polysaccharides, each of which reacts with antibody to one of the aforementioned unitypic strains and represents an unusual binary capsulated streptococcus.

Antigens, Bacterial↗
PubMed1973

Filamentous capsulated streptococci from the human respiratory tract. 3. Immunochemical studies of the cross-reactivity between the cell wall antigens of a filamentous streptococcus and of pneumococcus.

A cell wall-associated polysaccharide has been isolated from a noncapsulated mutant, 89R50, of the alpha-hemolytic filamentous streptococcus of provisional capsular type 89. Chemical analysis of the polysaccharide indicates that it consists of galactosamine, glucosamine, glucose, and phosphorus. Immunological cross-reactivity was observed between the streptococcal cell wall antigen and antibody to the cell wall-like capsular polysaccharide of pneumococcus. This cross-reactivity appears attributable to similarities in the teichoic acid moieties rather than in the mucopeptide moieties of the two polymers. No chemical or immunological differences were observed in the cell wall carbohydrate of the noncapsulated streptococcus, 89R50, and that of its capsulated progenitor. It is suggested that antibody to this cell wall polysaccharide might serve as a basis for grouping other filamentous strains of streptococci of diverse capsular types, the cell wall antigens of which show similar cross-reactivity with that of pneumococcus.

Antigens, Bacterial↗