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Biomedical subjects

J A Greene

Publications and source records attributed to J A Greene.

At least 37 records · Page 2Linked to original sources
PubMed1984

Epidemiology of rotavirus infection in broiler chickens: recognition of four serogroups.

In a longitudinal survey of 11 broiler flocks, rotavirus excretion was detected by direct electron microscopic examination of faeces in 10. In most of these flocks, rotavirus excretion was first detected during the third week of life. In some flocks, infection with 2 antigenically distinct serogroups of rotavirus was demonstrated. In a more detailed survey of a 34,000 bird broiler crop, rotavirus excretion was detected intermittently from 9-50 days. Infection with 4 different RNA electropherotypes of rotavirus occurred in waves with each wave of infection lasting about 1 week. Analysis of representatives of the 4 different electropherotypes by cross-immunofluorescence indicated that each electropherotype represented an antigenically distinct serogroup. Two of these serogroups were represented by the previously characterised Ch 1 and 132 chicken rotavirus isolates. The other 2 serogroups have not been previously recognised.

Animals↗
PubMed1984

Taste acuity and zinc status in chronic renal disease.

The malnutrition often associated with progressive renal failure may be related to a progressive deterioration of taste acuity. To determine the relationships between taste acuity, zinc status, and progressive renal failure, 27 subjects were divided into three groups and classified as having mild, moderate, or severe renal failure, with creatinine clearance of 41 to 75, 15 to 40, and 5 to 14 ml/minute, respectively. The subjects were evaluated for recognition taste thresholds for sour and sweet by the Cornsweet method. Serum, urinary, and salivary levels for zinc were determined by atomic absorption techniques. Food diaries were kept for two days; intakes of protein and zinc were calculated from the diaries. There was a significant (p less than .01) inverse relationship between creatinine clearance and recognition taste thresholds for sour and sweet. Serum zinc levels were significantly lower (p less than .01) for subjects with severe renal failure than for subjects with mild renal failure. The subjects who ingested more protein also ingested more zinc.

Adolescent↗
PubMed1978

Effect of shock strength on survival and acute cardiac damage induced by open-thorax defibrillation of dogs.

The safety of open thorax defibrillation with single damped sine-wave shocks and 6-cm-diameter electrodes was evaluated in healthy anesthetized dogs. Twenty-one dogs were allotted to 6 groups: Group A were nonshocked controls and groups B through F were given single shocks of 4-, 7-, 12-, 19-, or 32-fold, respectively, greater than a defibrillation threshold dose (30 mA/g of heart). Immediate postshock death resulted in group F dogs; group A through E dogs survived and were killed after 2 days. The incidence and severity of cardiac morphologic damage increased with shock strength (mild damage occurred in 1 of 3 dogs in group C and in 3 of 4 dogs in group D and severe damage occurred in 2 of 3 dogs in group E). The cardiac lesions were characterized grossly and microscopically. In dogs that died immediately after shocking, damage was apparent as pale circular zones of edema and myofibrillar degeneration in the ventricular free walls beneath the electrode placement sites on the cardiac surface. In the dogs that survived 2 days, the defibrillator-induced areas of myocardial necrosis and calcification were concentrated in arc or ringlike patterns beneath the periphery of the electrode placement sites. All dogs that were studied 2 days after shocking had mild fibrinous pericarditis. Postshock electrocardiographic changes were not good indicators of cardiac damage because the mild epicardial inflammatory reaction associated with the surgical procedure produced large ST and T wave changes which masked any changes associated with myocardial necrosis induced by the electric shocks. It was concluded that a substantial safety margin exists between the required defibrillation threshold shock dose and the large shocks required to produce marked cardiac damage or death in healthy dogs.

Animals↗