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Biomedical subjects

J A Goeken

Publications and source records attributed to J A Goeken.

59 records · Page 4Linked to original sources
PubMedDate not supplied

The safety of weekly plateletpheresis: effect on the donors' lymphocyte population.

Ten normal donors were monitored before, during, and after ten weekly donations of platelet concentrates prepared using the Haemonetics Model 30 blood processor. Analysis of these data indicates about a 20 per cent decrease of lymphocytes, with B cells being significantly decreased in half of these patients. While coincident immunoglobulin deficiencies did not develop in this short-term study, it is presumed that these would occur with a prolongation of platelet donations, as has been noted in previous studies. Additional studies are indicated to further delineate the lymphocyte depletion in normal platelet donors.

Adult↗
PubMedDate not supplied

Effects on immunity of multiple leukapheresis using a rapidly excreted analog of hydroxyethyl starch.

Immunologic studies were performed immediately before and after leukapheresis in five subjects undergoing four granulocyte donations (separated by 3- to 4-week intervals) using continuous-flow centrifugation leukapheresis with a rapidly excreted investigational analog of hydroxyethyl starch. Postleukapheresis donor blood lymphocyte counts and serum immunoglobulin levels decreased transiently, but significantly, during each procedure when compared with preleukapheresis values due simply to plasma volume expansion (acute effects of dilution). Possible long-term effects of repeated procedures were investigated by comparing preleukapheresis values before the first and fourth procedures. Total lymphocyte, total T-lymphocyte, and T4-lymphocyte counts, as well as serum immunoglobulin values and anti-A and B titers measured before the fourth procedure remained unchanged when compared with values obtained before beginning the series of procedures. In contrast, the B-lymphocyte count was decreased, the T8-lymphocyte count was increased, and the T4:T8 ratio was decreased due to the increase in the T8-lymphocyte count. Although statistically significant (p less than 0.05), all changes were slight, and clinically significant effects on donor humoral immunity could not be detected. The mechanisms involved, however, may be of biologic and therapeutic interest.

Antigens, Differentiation, T-Lymphocyte↗
PubMedDate not supplied

Effects of intensive granulocyte donation on donors and yields.

Thirteen HLA-identical bone marrow donors served as the sole source of daily granulocyte transfusions for respective marrow recipients during the period of severe neutropenia between transplantation and engraftment. They experienced 12 to 29 (median, 17) daily, continuous flow centrifugation leukapheresis procedures using hydroxyethyl starch, but no corticosteroids, with little serious difficulty. No immediate clinical reactions occurred in 90 percent of 228 procedures. Mild citrate reactions were noted in 9 percent, and only two procedures (0.8%) were discontinued due to severe reactions. Ten donors (77%) answered a questionnaire mailed weeks later, and six reported transient, late clinical adverse effects. Five had moderate dermatologic problems; one had minimal hypertension requiring no therapy. Donors were monitored daily for laboratory abnormalities while donating granulocytes. Hemoglobin concentration and platelet counts remained stable (autologous red cell transfusions had been given). Blood leukocyte counts gradually fell (p less than 0.05), particularly after 10 or more daily granulocyte donations, and this fall was associated with a decrease of about 33 percent in leukocyte yields. No attempts were made to improve yields by giving higher doses of hydroxyethyl starch or by corticosteroid stimulation. With primary emphasis on donor safety, it seems feasible for a few compatible donors to provide prolonged granulocyte transfusion support for designated patients. However, diminishing leukocyte yields may result from intensive, repeated leukapheresis.

Blood Donors↗