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Biomedical subjects

J A Cherry

Publications and source records attributed to J A Cherry.

67 records · Page 4Linked to original sources

Genetic analyses of gustation in the fowl.

Preferences towards quinine sulfate (QS) and dextrose (DEX) were tested in purelines and reciprocal crosses of two lines of chickens that had undergone 22 generations of selection for high and low juvenile body weight. Parental line-F1 comparisons provided evidence for non-additive genetic variation for hedonic sensitivity towards QS and DEX, though in opposite directions. Additive genetic variation appeared to influence the preference ratios for both stimuli at super-threshold concentrations. These results are discussed with regard to their evolutionary implications.

Animals↗

Correlated responses of chickens to selection for production of antibodies to sheep erythrocytes.

A bidirectional selection experiment was conducted to measure 5-day antibody titers to sheep erythrocytes in White Leghorn chickens. There was an immediate response to selection with significant differences between lines for the selected trait found in the S1 and all subsequent generations. Comparisons of S6, S7 and S8 generation females revealed differences between lines in disease resistance and in certain reproductive traits such as age at first egg, percentage hen-day egg production, percentage fertility and duration of fertility. The implications of these correlated responses are important to selection programs for general disease resistance.

Animals↗

Feeding and drinking response of young chicks to injections of serotonin into the lateral ventricle of the brain.

The effects of serotonin [5-hydroxytryptamine (5-HT)] on food intake and water consumption were investigated in young broiler chicks. Artificial cerebrospinal fluid containing either 0, 33, 67, or 100 microgram of 5-HT/10 microliter was injected into the lateral ventricle of either 24-hr fasted or fully fed chicks maintained in a thermoneutral environment with continuous lighting. Neither cannulation per se nor injection of 10 microliter of artificial cerebrospinal fluid had an effect on food intake or water consumption. Furthermore, food intake was not affected by 5-HT in fasted chicks. In fully fed chicks, however, food intake was significantly decreased by 67 and 100 microgram of 5-HT. Water consumption was affected by 5-HT injection in fully fed chicks. Results with fasted chicks remain equivocal. Data suggest that 5-HT may be involved in the neurohumoral control of food intake.

Animals↗

Food and water intake following injection of glucose into the lateral ventricle of the brain of broiler type chicks.

The effects of glucose and 2-deoxy-D-glucose (2-DG) on food intake and water consumption were investigated in broiler chicks. Artificial cerebrospinal fluid (aCSF) containing either glucose or the glucose antimetabolite 2-DG was injected into the lateral brain ventricles of fasted and satiated chicks. The satiated chicks received 10 microliters of CSF containing either 5 or 10% 2-DG, whereas those fasted for 24 hr received CSF with 10, 20, or 30% glucose (dextrose) in 10 microliters volumes. Chicks were maintained in a thermoneutral environment with continuous lighting. Intraventricular injections of 5 or 10% 2-DG had no significant effect on food intake or water consumption in the fully-fed chicks. In the 24-hr fasted chicks, 10% glucose had no effect on food or water intake. The decrease in food intake observed following the injection of 20 and 30% glucose appeared to be an osmotic effect. Based on results obtained, it was suggested that selection for increased growth rate, such as in broilers, may have resulted in a decrease in glucoreceptor sensitivity. Such a decrease in sensitivity could lead to an increased food intake as occurs in broilers.

Animals↗

Dietary cellulose, wheat bran, and fish meal in relation to hepatic lipids, serum lipids, and lipid excretion in laying hens.

Isonitrogenous diets supplemented with 10% cellulose, 10% wheat bran, or 10% fish meal were fed to White Leghorn hens from 245 to 322 days of age. Neither body weights nor egg production of the hens fed these diets differed significantly from those of controls fed a corn-soybean meal basal diet. Supplemental fish meal and wheat bran did not significantly affect liver weight or liver lipids, although serum lipids of the hens fed the diet containing fish meal were significantly lower than those of the hens fed the basal diet. The hens fed the cellulose-supplemented diet, however, exhibited significantly decreased liver weights, serum lipids, and liver lipids, which were accompanied by a significant increase in excreta lipid. It is speculated that lipid excretion may be involved in some dietary induced alterations in the hepatic lipid concentration of laying chickens.

Animals↗

Jejunal glucose absorption in selected high and low weight lines of domestic fowl.

Intestinal glucose uptake was measured in normal and dwarf chickens from lines selected fro high and low body weight. Jejunal tissue rings were excised from 9-week-old chicks and incubated in a medium containing .002 M glucose. Significantly more glucose (micromoles per gram dry tissue weight) was absorbed by tissues from the low weight line when compared to tissues from the high weight line. The dwarf gene, dw, had no effect on glucose Uptake.

Animals↗

Dwarfism in diverse genetic backgrounds. 4. Effects of dietary energy.

The interactions in the response of dwarf and normal genotypes to dietary energy were studied in lines of chickens divergently selected for high or low juvenile body weight. The genetic background of the dwarf genotype significantly affected body weights, egg production, and egg quality. The dietary energy levels used did not significantly affect these traits or the differences due to genetic background. Dwarf chickens were able to adapt to wide ranges of dietary energy when the diets contained sufficient crude protein.

Animal Feed↗

Repeatabilities of feed and water consumption of growing chickens.

Daily feed and water consumptions were obtained within 7-day periods for dwarf and normal males from lines selected for high and low juvenile body weight from 21 to 90 days of age. No significant differences were found among populations for the repeatabilities, and three measurements per week were sufficient to obtain an accurate estimate of feed and water consumption of growing chickens.

Animals↗

Vertical cross contamination of trichloroethylene in a borehole in fractured sandstone.

Boreholes drilled through contaminated zones in fractured rock create the potential for vertical movement of contaminated ground water between fractures. The usual assumption is that purging eliminates cross contamination; however, the results of a field study conducted in a trichloroethylene (TCE) plume in fractured sandstone with a mean matrix porosity of 13% demonstrates that matrix-diffusion effects can be strong and persistent. A deep borehole was drilled to 110 m below ground surface (mbgs) near a shallow bedrock well containing high TCE concentrations. The borehole was cored continuously to collect closely spaced samples of rock for analysis of TCE concentrations. Geophysical logging and flowmetering were conducted in the open borehole, and a removable multilevel monitoring system was installed to provide hydraulic-head and ground water samples from discrete fracture zones. The borehole was later reamed to complete a well screened from 89 to 100 mbgs; persistent TCE concentrations at this depth ranged from 2100 to 33,000 microg/L. Rock-core analyses, combined with the other types of borehole information, show that nearly all of this deep contamination was due to the lingering effects of the downward flow of dissolved TCE from shallower depths during the few days of open-hole conditions that existed prior to installation of the multilevel system. This study demonstrates that transfer of contaminant mass to the matrix by diffusion can cause severe cross contamination effects in sedimentary rocks, but these effects generally are not identified from information normally obtained in fractured-rock investigations, resulting in potential misinterpretation of site conditions.

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