Biomedical subjects

Iu V Chumachenko

Publications and source records attributed to Iu V Chumachenko.

3 recordsLinked to original sources

PubMedDate not supplied

[High-voltage electrophoresis and its application in combination with other methods for protein structure studies].

The method of high-voltage paper electrophoresis may be applied not only for peptide separation, but also in modifications and combinations with other methods, so, by means of aminoethylation and maleylation it is possible to broaden or narrow the range of trypsin action. This, in its turn, makes it possible to isolate preparatively lysin- and arginine-containing peptides, oxidation with performic acid enables the thyol-containing fragments to be isolated and application of carboxypeptidase A-C-terminal peptide of protein. When studying the primary structure of proteins the method has already found its widest application but with an increase in the number of methods of protein specific modification its potentiabilities will be even wider.

Carboxypeptidases↗

PubMedDate not supplied

[Comparative characteristics of catalase from the fungus Penicillium vitale, which is synthesized under different nutritional conditions].

A comparative study of properties (absorption spectra, thermostability, pH optimum, polyacrylamide gel electrophoresis, DEAE-cellulose separation) and structure (amino acid composition, finger-prints, carbohydrate composition) was performed for P. vitale catalase synthesized under different medium conditions. In all cases the results were similar. The only difference occured in the amount of synthesized proteins. A conclusion is drawn that under different nourishing conditions of the fungus the properties and structure of the catalase are unchanged, but the regulatory mechanisms of catalase and glucosooxidate synthesis undergo changes.

Amino Acids↗

PubMedDate not supplied

[Simple technic for multiple small sample lyophilization].

This simple and easily reproducible technique does not pretend to be new in principle but is still very convenient for drying numerous samples. The advantage of the technique is based on preliminary sample freezing just before dessication. The tubes must be sealed with needle punctured cellophane to avoid the samples squeezing out by ice-entraped air bubbles. This approach is especially convenient for protein structure investigations when it is necessary to dry and accumulate peptides from the maps on high-voltage electrophoresis and chromatography. In a 1.5-litre dessicator some 20 ml of sample can be dried out without reloading.

Freeze Drying↗