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Biomedical subjects

Iu R Khrust

Publications and source records attributed to Iu R Khrust.

24 records · Page 2Linked to original sources
PubMedDate not supplied

[Phorbol ester disrupts the cleavage pattern in sea urchin embryos].

Phorbol-12-myristate-13-acetate (PMA) treatment (9-170 nM) of S. mirabilis embryos from the fertilization to the IV cleavage division resulted in morphogenesis disturbances, such as decrease of the intercellular contacts' extent of blastomeres during the first cleavage divisions, inhibition of micromere formation, and formation of multiple blastulae. After washing from PMA embryos remained viable up to the pluteus stage. Morphogenetic disturbances were not accompanied by changes of intracellular pH or mechanical properties of blastomeres' surface during cleavage. Protein kinase C is suggested to control adhesion of the cell surface and intracellular nucleus movements.

Animals↗
PubMedDate not supplied

[Effect of local anesthetics and phorbol ester on intracellular pH and rate of development of sea urchin embryos].

The unfertilized eggs of sand-dollars (Scaphechinus mirabilis) were treated with local anesthetics (novocaine, lidocaine, trimecaine) or phorbol ester for 10 min. The treatment with the local anesthetics increased pH of the cytoplasm in the unfertilized eggs (pHc) and changed the value of the pHc increment in response to fertilization, as compared with the control eggs. In the treated embryos, the onset of immigration of the primary mesenchyme cells was delayed but the hatching was accelerated. The phorbol ester treatment rapidly accelerated development from gastrulation on and practically did not affect the preceding stages.

Anesthetics, Local↗