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Biomedical subjects

Iu M Azizov

Publications and source records attributed to Iu M Azizov.

At least 19 recordsLinked to original sources
PubMed1998

[Metabolic effects of intermittent hemofiltration in patients with acute renal failure].

AIM: Investigation of postaggressive reaction of metabolism on intermitten hemofiltration (IHF) in patients with acute renal failure (ARF). The speed of generation of urea, creatinine and oligopeptides was estimated in the course of (IHF) and in interval between the procedures. Hormonal stress was evaluated by concentration of hydrocortisone in plasma and and filtrate. MATERIALS AND METHODS: The trial covered 36 patients with oliguric ARF (21 males and 15 females) for whom IHF was the main method of replacement renal therapy. The kinetic modelling accounted for distinctive distribution of markers in body fluids. Statistical processing was made according to Student-Fisher T-criterion. RESULTS: IHF runs with intensive proteinolysis with acceleration of oligopeptides generation by 810%, degradation of large quantities of creatine triphosphate and 770%-accelerated production of creatinine, with protein catabolism and 440%-accelerated production of urea. Hydrocortisone blood levels rise about two-fold despite intensive elimination of the hormone with filtrate. Between speeds of IHF, generation of creatinine and secretion a direct correlation was found. This indicates the importance of the perfusion rate for induction of the stress and catabolism. Background hydrocortisone Irvrl has significant effects only on urea accumulation rates in IHF-free intervals. However, in the course of IHF, generation of urea and oligopeptides is to a great extent independent of hydrocortisone secretion. CONCLUSION: Application of IHF in ARF patients results in rather strong stress with enhanced proteinolysis and protein catabolism. To compensate protein loss associated with only urea generation, additional administration of minimum 60 g of plasma or amino acids is required.

Acute Kidney Injury↗
PubMed1991

[Acid-soluble fractions of the blood in patients with nonspecific diseases of the respiratory organs].

A method permitting the differentiation between the types of pulmonary inflammatory processes in patients with nonspecific diseases of the respiratory organs by the parameters of blood plasma acid-soluble fraction is suggested. If the inflammation is of an infectious origin (pneumonia, chronic bronchitis), the ratio of low-molecular to high-molecular fractions of the blood plasma acid-soluble fraction is 4.2 +/- 1.8. In allergic inflammations (bronchial asthma) this ratio is 9.0 +/- 5.8. The results of this test were in line with clinical laboratory and x-ray findings in 86.7% of the examinees. In complex with other methods of examination, the described technique may be used for the differential diagnosis of allergic and infectious inflammations in patients with respiratory diseases.

Acute Disease↗
PubMed1990

[The products of blood coagulation and fibrinolysis and their effect on pulmonary gas-exchange function].

The authors' and reported data have demonstrated the effect of hemocoagulation and fibrinolysis products on gas exchange pulmonary function in patients who suffered surgical intervention. The results of examining 20 patients with gastric carcinoma are provided. It has been established that even before operation the patients manifested the chronic pattern of the disseminated intravascular coagulation (DIC) syndrome, whose intensity increased in the postoperative period. The rise in the blood of soluble complexes of fibrin monomer (SCFM) and fibrin degradation products (FDP) formed during intravascular coagulation was established to play an important role in the impairment of gas exchange pulmonary function in the postoperative period in patients with the chronic pattern of the DIC syndrome. A possible mechanism by which SCFM and FDP influence pulmonary gas exchange is reviewed.

Aged↗
PubMed1990

[The possible pathways for the formation of a pool of an acid-soluble blood plasma fraction in patients with diffuse suppurative peritonitis].

In patients with diffuse purulent peritonitis, the total pool of an acid-soluble blood plasma fraction (ABPF) considerably increases as compared to that in normals (donors). The present paper is concerned with the role of proteolytic activity and lysis of bacterial cells and cells of different human tissues in the formation of the ABPF in patients with peritonitis. For this purpose the normals' blood and its separate components were treated with various enzymes (trypsin, pronase, chemotrypsin, papain, elastase, alpha-amylase) to measure the ABPF. The pattern of changes detectable on such a treatment was compared with the tendency of changes seen in patients with peritonitis. Measurements were also made of acid-soluble fractions in suspensions of bacterial cells and cells of the pancreas, liver and heart muscle tissue of man. It has been found that on proteolysis of plasma proteins and membrane proteins of blood cells there form products contained by the ABPF. Such products formed in the greatest amount as a result of treatment with pronase. Products of the lysis of bacterial cells may be also contained by the ABPF of peritonitis patients. The possibility of detecting proteolytic degradation and cellular disintegration according to the spectral characteristics of separate fractions of the ABPF is discussed.

Bacteriolysis↗
PubMed1989

[Protein C].

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Blood Coagulation↗
PubMed1981

[Neutral glycolipids of the calf thymus and normal and leukemic bovine lymphocytes].

The composition and structure of the neutral glycolipids of calf thymus and of lymphocytes isolated from the blood, lymph, lymph nodes and spleen of normal and leukemic cows were investigated. The main neutral glycolipids of all lymphocytes studied were identified as glucosyl, lactosyl and digalactosylglucosylceramides. The relative amounts of these glycolipids were found to depend on lymphocyte localization. Leukemic lymphocytes showed differences in the glycolipid composition in comparison with normal lymphocytes: in all preparations the level of glucosylceramide increased with a concomitant decrease of the amounts of di-and trihexaosylceramides. For leukemic blood lymphocytes the monohexaosyl-/dihexaosylceramide ratio was found to increase at increasing lymphocyte concentrations in the blood.

Animals↗
PubMed1980

[Structure of gangliosides of normal and leukemic bovine blood lymphocytes].

The structure of main gangliosides from normal and leukemic bovine blood lymphocytes was established. N-Glycoloylhematoside and a ganglioside containing N-glycoloylneuraminic acid and an oligosaccharide chain identical to that of erythrocyte sialosylparagloboside were found to be present both in normal and in leukemic bovine blood lymphocytes. In addition, the leukemic lymphocytes contained a ganglioside identical to N-acetylneuraminosylparagloboside and at least two disialosyllactosylceramides containing N-acetyl- and N-glycoloylneuraminic acids. It was assumed that lymphocyte maturation is accompanied by a loss of disialosyllactosylceramide and that the latter influences the immunological properties of the apparently less mature leukemic peripheral lymphocytes.

Animals↗
PubMed1979

[Tissue specificity of the decrease of cattle lymphocyte DNA methylation during chronic lymphoid leukemia].

It has been found that the content of m5C in the DNA preparations tested have been revealed. The DNAs from normal and leukemic lymphocytes of blood, lymphonodi and spleen differ in ther acceptor ability in the reaction of heterologous methylation in vitro, induced by DNA-methylase from Enterobacter cloacea in the presence of [3H-methyl]S-adenosyl methionine: the ratio of radioactivities in methylated cytosine and adenine residues (m5C/m6A) in leukemic lymphocyte DNA is much lower than in healthy animals' lymphocytes. The decrease in the methylation of DNAs from various lymphoid organs of animals with chronic lymphoid leukemia is well correlated with the impairment. No significant changes of the m5C level and the acceptor ability of the in vitro reaction of heterologous methylation of cow lymph lymphocyte DNA have been observed. The data obtained may be interpreted in terms of tissue (cell) specificity or differences in the degree of DNA methylation under conditions of chronic lymphoid leukemia. It is assumed that the changes in DNA methylation may underlie the disturbances in the regulation of activity of the leukemic cell genetic mechanisms.

Animals↗
PubMed1979

[Ganglioside composition of normal and leukemic bovine lymphocytes].

The ganglioside composition of bovine peripheral lymphocytes was shown to change sharply under lymphoid leukemia. In normal lymph, lymph nodes, spleen and blood lymphocytes the major ganglioside is N-glycolylhematoside, whereas in calf thymus lymphocytes appreciable amounts of more polar components (GM1- and GD1a-like gangliosides) were found. In leukemic lymphocytes isolated from the same tissues the hematoside content is decreased, while the amount of more polar gangliosides is increased. Possible causes of the altered ganglioside pattern in leukemic lymphocytes are discussed.

Animals↗
PubMed1979

[Intragenome distribution of 5-methyl cytosine and reassociation kinetics of cow blood lymphocyte DNA under normal conditions and in chronic lymphoid leukemia].

The DNA from cow blood lymphocytes is methylated in a varying degree: the maximal content of 5-methyl cytosine (2,3 mol%) is found in the "instantly" renaturating sequences (Cot lett than 10(-4)), a relatively large amount (1,4 mol%)--in moderately repeated sequences (Cot = 10(-4)--400) and the minimal amount (0,9 mol%) in the unique sequences (Cot greater than 400). In their reassociation kinetics, GC-content and other physico-chemical properties the blood lymphocyte DNA of the controls and of animals with chronic lymphoid leukemia appear to be similar. Consequently, the genome organization of leukemic animals does not change significantly; a considerable decrease of 5-methyl cytosine of lymphocyte DNA in lymphoid leukemia parallels the decrease of genome methylation in the leukemic cells. This decrease does not affect the unique sequences, but involves all types of repeated sequences (moderately and frequently repeated ones and palindromes). It is assumed that the specific disturbances in genome methylation under lymphoid leukemia may be a cause of transcription deficiences and cell transformations.

Animals↗
PubMed1978

[Phospholipid composition of lymphocytes from normal and leukemic blood, lymph, lymph nodes and spleen of cattle].

The phospholipid composition and cholesterol content of lymphocytes from bovine blood, lymph, lymph-nodes and spleen were studied. The cholesterol/phospholipid molar ratio in the normal lymphocytes of blood and spleen was found to be about two times higher than that in the normal lymphocytes from lymph and lymph-nodes. On lymphocytic leukemia the cholesterol/phospholipid molar ratio of the blood lymphocytes decreased sharply, whereas that in lymph and lymph-nodes lymphocytes was unaffected.

Animals↗
PubMed1978

[Changes in the specificity of DNA methylation in cattle blood lymphocytes under chronic lymphoid leukemia].

Under conditions of chronic (spontaneous) lympholeucosis the amount of 5-methylcytosine in cattle blood lymphocyte DNA is decreased approximately by 30%. No other changes in the DNA (e. g. GC-content, Tm, amount of pyrimidine sequences differing in their lengths and composition) were observed. Thus, the decrease in the amount of 5-methylcytosine in lymphocyte DNA is due to a decrease in DNA methylation. This decrease is non-random and involves mainly the Pu-m5C-Pu sequences without affecting the long pyrimidine blocks. In nuclear extracts from lymphocytes of healthy animals the DNA-methylase activity having an optimum at pH 6,0 was found; the DNA-methylase activities found in the nuclear extracts of leukaemic cow lymphocytes had their optima at pH 5,5 and 7,5. In vitro the DNA-methylase activities of leukaemic lymphocytes nuclei methylate the cytosine residues of DNA in other sequences than enzyme(s) of the extracts from normal lymphocyte nuclei. Changes in the pattern of DNA-methylase activities as well as the decrease and distortions in the character of DNA methylation may underlie the disturbances in the regulation of transcription of genome and cause the transformation of cells under conditions of lympholeucosis.

Animals↗
PubMedDate not supplied

[The oxidative modification of blood plasma proteins in patients in critical states].

Current concepts on the formation and biological activity of activated oxygen forms (AOF) in humans are discussed. The main AOF types are produced as a result of consecutive single-electron recovery of molecular oxygen (O2) and are more reactive than O2. AOF are initially normal components of cellular metabolism with certain biological functions. Their reactive aggressiveness is regulated by a potent antioxidant system which is present in any live organism. In disease this balance is distorted towards uncontrolled AOF generation and formation of oxidative stress, when AOF impair all biological structures, including proteins. Unregulated modification of proteins by AOF results in loss of protein biological activities (enzymatic, receptor, transporting function, etc.). Moreover, oxidative modification of proteins generates new antigens and provokes immune response. The authors present experimental data which confirm significant modification of plasma proteins in critical patients. The role of the detrimental effect of AOF on proteins in the formation of critical states deserves special studies.

Animals↗