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Biomedical subjects

Iu A Sorokin

Publications and source records attributed to Iu A Sorokin.

17 recordsLinked to original sources
PubMed1980

[Organelle-specific enzymatic activity as an index in evaluating the quality of liver preservation].

The dynamics of changes of the general, "non-sedimenting" activity of organelle-specific enzymes as well as their activity in perfusate during hypothermal conservation of the liver were studied in 77 experiments by the method of continuous and discontinuous perfusion, without perfusion and during the period of normal thermally artificial blood circulation after 3 hours of the organ conservation. The complex of enzyme tests including the assessment of "non-sedimenting activity" of mitochondrial glutamatdehydrogenase and lysosomal acid desoxiribonuclease during the conservation period was found to be an adequate criterion of the liver conservation quality. After the recovery of the artificial blood flow in the conservated organ the conservation quality is also assessed by their activity in the perfusate, in addition to determination of urokinase activity in the perfusate.

Animals↗
PubMed1980

[Conservation of the functional capacity of the isolated liver during its hypothermic perfusion with eryhem: experimental study].

The functional capacity of the dog's isolated liver was studied in experiment during a 3-hour period of conservation by the method of hypothermal (+8 degrees) perfusion with a solution containing free hemoglobin in concentration 1,8--2,0 g% and under conditions of the following normo-thermal artificial circulation of blood in the isolated organ. It was established that the presence of erygem as a component of the perfusate enables oxygen requirements of the preserved liver to be fully satisfied.

Animals↗
PubMed1979

[Hemoperfusion through adsorbents as a method of preoperative preparation of patients with severe form of mechanical jaundice].

The method of haemoperfusion through the ion exchange resin and active carbon was used 15 times in 13 patients with heavy forms of mechanical subhepatogenous jaundice. The haemoperfusion was used as a method of preoperative preparation for reduction of hyperbilirubinemia and cholemic intoxication and for prevention of possible postoperative complications. One patient recovered. One patient died of progressing hepatic insufficiency. The rest of the patients were subjected to surgical interventions for the main disease.

Charcoal↗
PubMed1978

[Surgical aspects of a temporary heterotopic transplantation of the liver].

The ways of settling some surgical aspects of a temporary heterotopic transplantation of the liver are analysed in the article. According to the obtained data the placement of the graft into the iliac zone of the abdominal cavity is the most convenient for a temporary heterotopic transplantation of an additional liver. An adequate vascularization of the additional organ can be effected via hepatic artery, which simplifies considerably the removal and replacement of this organ. For a free placement of the graft the weight of the donor should be 2 or 3 times less than that of the recipient.

Animals↗
PubMed1978

[Effect of hypothermia on metabolism in the liver during its preservation].

Twenty experiments were conducted on dogs. The effect of hypothermia of different degree (from 18 to 20 degrees C and from 4 to 6 degrees C) on the carbohydrate metabolism and the extent of solubilization of hepatic enzymes (lactate dehydrogenase, glutamate dehydrogenase, urokaninase, DNA-ase, glucose-6-phosphatase) in prefusion-free preservation of the liver was studied. The preservation efficacy was assessed during the subsequent two-hour normothermic perfusion. A marked solubilization of the enzymes under study followed preservation of the liver at 18--20 degrees C; this indicated the loss of intactness of the cell membranes during the preservation. A moderate expenditure of the glycogen stores in the liver, and of sugar in the perfusate followed preservation of the liver at a temperature of 4--6 degrees C; this suggested an even suppression of hepatic metabolism and the prevalence of normal tissue respiration over glycolysis in the restoration of circulation in the liver.

Animals↗
PubMed1976

[Absorptive-excretory function of isolated dog liver during its perfusion].

Twenty experiments were performed on mongrel dogs. The absorptive and excretory function of the liver in the intact organism and during its isolated perfusion through the portal vein with the use of extracorporeal circulation was studied by means of the bromsulfaline test. The absorptive function of the perfused liver proved to decrease by 50-60 per cent. The rate of bile elimination and plasma clearance was 5-6 times higher at the beginning of the experiment in the intact organism. Later on, the difference in the degree of retention of the hepatic excretory function was reduced. Hypoxia connected with denervation, hepatectomy and the isolated perfusion proper apparently served as the principal cause of reduction of the absorptive-excretory function of the isolated liver.

Animals↗
PubMed1976

[Solution for liver preservation].

The author suggested a solution for preservation of the liver. The specific feature of the solution is that it contains special substances of carbohydrate and protein nature which, requiring no complicated metabolic transformations, can easily be involved in the liver metabolism during the period of its preservation. Observations have demonstrated that the preserving substance suggested by the author is much better than analogous solutions employed in control experiments.

Animals↗