Biomedical subjects

Irfan Bayram

Publications and source records attributed to Irfan Bayram.

22 records · Page 2Linked to original sources

PubMed2003

[The role of peritoneal aspiration cytology diagnosis of acute appendicitis].

BACKGROUND: The purpose of this study is to search the role of peritoneal aspiration cytology in acute appendicitis. METHODS: Peritoneal aspiration cytology was (PAC) conducted for fifty suspected acute appendicitis patients before their operations. PAC findings was compared with histopathologic results. RESULTS: Negative appendicectomy rate according to clinical diagnosis was 20%. PAC was positive in 32 and negative in 18 patients. 93.75% PAC (+) patients had acute appendicitis, 44.4% PAC (-) patients had normal appendices. The false positive rate was 6.2% and the false negative rate was 55.6%. The sensitivity, the specificity, the positive predictive value, the negative predictive value of PAC for the diagnosis of acute appendicitis were 75%, 80%, 93.8%, 44.4%, respectively and the accuracy was 76%. CONCLUSION: PAC positive results support the diagnosis of acute appendicitis strongly. However, PAC (-) results don't exclude the diagnosis of acute appendicitis.

Acute Disease↗

PubMed2003

Tissue transglutaminase expression in duodenal mucosa of patients with celiac disease and of normal subjects.

BACKGROUND/AIMS: Our aim in the present study was to investigate tissue transglutaminase expression by immunohistochemistry in duodenal mucosa of patients with celiac disease. METHODS: A total of twelve patients with celiac disease were examined. The patients had different clinical and histopathologic degrees of severity and responded to gluten withdrawal with clinical improvement. Ten subjects with various unrelated diseases served as controls. Serum endomysium antibodies were measured by an indirect immunofluorescence method using a commercial kit. Duodenal biopsy specimens were stained with hematoxylin and eosin. Immunohistochemical staining for anti-tissue transglutaminase antibodies was performed using commercial kit. RESULTS: Serum endomysium antibodies and evaluation of small bowel biopsy specimens were normal in all control subjects. However, serum endomysium antibodies were positive in all of the celiac patients. Immunohistochemical staining pattern of duodenal biopsy specimen performed using anti-tissue transglutaminase antibodies was similar in celiac patients and control subjects. CONCLUSION: Tissue transglutaminase expression by immunohistochemical methods in untreated celiac mucosa is not suitable for diagnosis of celiac disease.

Adult↗

PubMedDate not supplied

Prognostic factors and COX-2 expression in advanced stage esophageal squamous cell carcinoma.

Cyclooxygenase-2 (COX-2) is overexpressed in various types of human malignancies, including squamous cell carcinomas of the esophagus, but its clinicopathologic role in esophageal squamous cell carcinoma (ESCC) remains unclear. The aim of this study was to analyze expression of COX-2 in ESCC and to correlate this expression with clinicopathologic parameters and survival. From 1999 to 2003, endoscopic tissue samples from 110 patients with ESCC were collected for analysis. COX-2 expression was examined through immunohistochemical staining. Clinicopathologic data were analyzed to verify significance. COX-2 expression was detected in 50 of 110 ESCC specimens (45%). COX-2 expression was negative to weak in 73% (COX-2 low) and moderate to strong in 27% (COX-2 high) of tumors. Statistical differences between COX-2 high and COX-2 low were found according to status of the stage (stage IVM1a/IVM1b) (P=.001): cancer antigen (CA) 19-9 (normal/high) (P%.011), CA 12-5 (normal/high) (P=.011), and CA 15-3 (normal/high) (P=.035). Survival was significantly reduced among patients with high COX-2 expression (median overall survival, 3 mo) when compared with the COX-2 low group (median overall survival, 6 mo) (P=.0001). In the univariate analysis, age, body mass index, stage, COX-2, lactate dehydrogenase, CA 12-5, and CA 15-3 were significant factors for survival. With the use of COX regression analysis, only stage (P=.000), COX-2 (P=.000), lactate dehydrogenase (P=.023), and CA 15-3 (P=.002) were independent prognostic factors. Results showed that in patients with ESCC, COX-2 overexpression was significantly correlated with visceral metastases IVM1b). COX-2 overexpression is an unfavorable prognostic factor in ESCC.

Adult↗

PubMedDate not supplied

Local irritant effects of topical oral sprays on oral mucosa in mice.

Topical oral sprays are frequently used to prevent and manage oropharyngeal inflammation and lesions. This study investigated the histopathologic changes noted in the oral mucosa of mice after topical application of 3 widely prescribed antibacterial products. The 25 animals were divided into 5 groups and treated for 10 days with 2 sprays daily, as follows: group 1-chlorhexidine gluconate 0.12% + benzydamine hydrochloride 0.15%; group 2-benzydamine 0.27 mg/0.18 mL x 30 mL; group 3-chlorhexidine 0.2%; group 4-fusafungine 1%; and group 5 (cohort)-physiologic serum. On day 10 after drug administration, biopsy specimens were taken from the oropharyngeal mucosa of the tongue, the cheek mucosa, and the tongue base; these were examined under a light microscope and were classified as normal or pathologic. All topical oral sprays produced some degree of histopathologic change, such as hyperplasia, fibrosis, low-grade dysplasia, congestion, or edema. The local irritant effects of topical oral sprays should be considered when treatment is selected for patients with oropharyngeal disorder.

Administration, Oral↗