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Biomedical subjects

Ichiro Katayama

Publications and source records attributed to Ichiro Katayama.

46 records · Page 3Linked to original sources
PubMed2002

Spontaneous IL-8 production by CD4(+), CD7(+) leukemia cells in erythrodermic Sézary syndrome.

Sézary syndrome has been described as the leukemic form of cutaneous T-cell lymphoma, defined by the presence of CD4(+) T-cells with cerebriform nuclei in the peripheral blood, erythroderma and lymphadenopathy with occasional involvement to lymph nodes, and visceral organs according to course. We report a 48-year-old woman with Sézary syndrome. Most of peripheral blood mononuclear cells (PBMC) showed CD4(+), CD7(+) surface marker while infiltrating cells to the skin consisted of the same percentage of CD4/CD8 cells. CD4(+) cells predominantly expressed IL-8 both in PBMC and the skin, and generated significant amount of IL-8. In contrast, lesional keratinocytes showed only a trace amount of IL-8 when compared to strong expression by infiltrating CD4(+) and CD8(+) cells. These studies suggested that leukemia cell-derived cytokines such as IL-8 might play some role in the induction of erythrodermic features of Sézary syndrome.

Antigens, CD7↗
PubMed2002

Substance P induced preprotachykinin-a mRNA, neutral endopeptidase mRNA and substance P in cultured normal fibroblasts.

In certain skin diseases, stress can modulate the induction and/or progression of cutaneous manifestations. However, little is known about the circuit in neuroendocrine and in the immune systems of the skin. To address this question, we have analyzed the regulatory mechanisms of autocrine induction of substance P (SP) by cultured normal human fibroblasts that compose the major population of the skin and might augment stress-induced skin inflammatory responses. In nonstimulated conditions, normal fibroblasts express a moderate amount of preprotachykinin-A (PPT-A), a precursor of SP mRNA, and exogenous SP significantly upregulated PPT-A mRNA expression. Maximum response of SP peptide and SP mRNA in fibroblasts was observed 1-3 h after stimulation with SP. In contrast, the expression of neutral endopeptidase (NEP), a cell surface peptide with hydrolyzing activity of SP, was increased in fibroblasts stimulated with SP after 24 h. The administration of NEP inhibitor (phosphoramidon) to the fibroblasts induced higher SP production. In addition, the neurokinin (NK) receptor antagonists (spantide, FK224 and FK888) and protein synthesis inhibitor (cycloheximide) inhibited SP production by 30-40% of control response. In immunostaining study, specific cytoplasmic staining of SP was observed in fibroblasts stimulated with SP. Finally, we confirmed that the nucleotide sequence of the PPT-A expressed in fibroblasts perfectly corresponded to the gene bank human PPT-A cDNA. This is the first report that SP mRNA, NEP mRNA and SP peptide can be induced by normal human skin fibroblasts in response to exogenous SP, and that fibroblast-derived SP might play an important role in the induction and acceleration of certain cutaneous diseases.

Cells, Cultured↗
PubMedDate not supplied

High-concentration (20 mug/g) tacalcitol ointment therapy on refractory psoriasis vulgaris with low response to topical corticosteroids.

The efficacy and safety of the application of high-concentration (20 mug/g) tacalcitol ointment once daily for 12 weeks to psoriasis vulgaris lesions which showed low response to topical corticosteroids, were evaluated in a prospective, multicenter, open-label study. Eighty patients were enrolled in the safety analysis of the test drug, and 54 of the 80 patients in the efficacy analysis. The efficacy rate based on the number of cases graded as "moderate improvement" or better in the final global improvement rating of the 54 cases included in the efficacy analysis, was 88.9% (95% CI: 77.4-95.8%). Significant improvement in erythema, thickness, and scaling was observed from 2 weeks of treatment onward (p < 0.001). Five local adverse reactions (2 events of irritation, 2 events of itching, and 1 event of redness) were observed in 3 of the 80 patients included in the safety analysis. There were no significant changes in mean serum calcium values. Tacalcitol 20 mug/g ointment is concluded to be effective and safe for the treatment of refractory psoriasis vulgaris with low response to topical corticosteroids.

Administration, Topical↗
PubMedDate not supplied

Localized argyria 20-years after embedding of acupuncture needles.

We report a 66-year-old woman with localized argyria caused by embedding of acupuncture needles. Ten years after she had received acupuncture, she noticed two asymptomatic bluish macules on her right arm. A biopsied specimen from the macule revealed many brownish-black granules mainly located around the sweat glands and the blood vessels in the dermis. The X-ray examination of the extremities revealed numerous needle-like fragments around her extremities. "Embedding of needles" induces some serious adverse events. We should know the adverse events for the safety and health of patients.

Acupuncture Therapy↗
PubMedDate not supplied

Open trial of topical tacalcitol [1 alpha 24(OH)2D3] and solar irradiation for vitiligo vulgaris: upregulation of c-Kit mRNA by cultured melanocytes.

Vitiligo vulgaris is a common skin disease, however some cases show poor clinical responses to topical steroid ointment or PUVA therapy. Such regimens are generally avoided in the treatment of facial lesions or in pediatric cases because of the undesirable side effects. To confirm the excellent response to combination therapy with topical vitamin D3 ointment and solar irradiation for vitiligo achieved in the initial patients, we conducted an open trial on other patients, most of whom had poor clinical responses to the prior therapies. Fifteen patients (9 men and 6 women) with vitiligo vulgaris were enrolled in this study. Each patient was instructed to sunbathe for 30 minutes within 1 hour after topical application of the tacalcitol [1 alpha 24(OH)(2)D(3)] ointment or cream to the skin lesions every day. Six of 15 patients showed a fair and excellent clinical response to the combination therapy (more than 30% clearance of the vitiligo). The clinical effect was more apparent in patients with a history of less than 5 years of vitiligo (4 of 6 cases) in contrast to those with a history of more than 5 years (2 of 9 cases). In vitro experiments revealed that tacalcitol upregulated the expression of c-Kit mRNA by melanocytes irradiated with linear polarized infrared, UVA or short period solar irradiation. These results suggest that combination therapy with topical vitamin D(3) ointment and solar irradiation can be used as an alternate therapy for vitiligo vulgaris.

Administration, Cutaneous↗
PubMedDate not supplied

Role of mast cells in dermatofibroma: recent viewpoints into the pathogenesis.

Mast cells are often discussed to play an important role in the tissue fibrotic process, because increased numbers of mast cells are found in the fibrous lesions. Recent evidence has revealed that mast cells are a rich source of cytokines or mediators, which are supposed to play a crucial role in altering the environmental extracellular matrix, leading to fibrosis. Dermatofibromas (DFs) are benign tumors histologically characterized by local fibroblast proliferation. It has been demonstrated that multiple DFs occur in patients with autoimmune diseases or under immunosuppressive therapy, implying that DFs are reactive tumors, rather than true neoplasms, at least in one aspect. Increased numbers of mast cells are also found in both solitary and multiple DFs, in particular in the layers between the DF lesion and the overlying epidermis. The presence of mast cells could be significant in the induction of several histopathologic changes, including acanthosis of the overlying epidermis, basal melanosis, and possibly mononuclear cell recruitment, in DF. Further elucidation of the role of mast cells may lend support to the understanding of the mechanism of DF.

Autoimmune Diseases↗
PubMedDate not supplied

Basaloid follicular hamartoma with eruptive milia and hypohidrosis: is there a pathogenic relationship ?

We report a sporadic case of eruptive milia with histopathological features of basaloid follicular hamartoma which developed in an 8 year-old Japanese girl. Multiple milia and comedo-like eruptions were present at birth and gradually increased in number and spread over the extremities. Histopathologically, keratotic cysts with trichilemmal keratinization and features of basaloid follicular hamartoma were observed without any histological findings of basal cell epithelioma or trichoepithelioma. Reduced sweating was observed after iontophoretically applied acetylcholine on the forearm. Nevus of Ota and thyroid goiter were complications.

Child↗
PubMedDate not supplied

Olmsted syndrome with squamous cell carcinoma of extremities and adenocarcinoma of the lung: failure to detect loricrin gene mutation.

Olmsted syndrome is an uncommon disorder of keratinization that presents mutilating palmoplantar keratoderma, periorificial hyperkeratosis, leukokeratosis and alopecia. We report a new case of this rare syndrome diagnosed in 48-year-old woman who developed several squamous cell carcinomas of limbs and adenocarcinoma of the lung. She has been followed up for about 40 years and osteolytic changes of the fingers and toes accompanied the keratinizing disorder and squamous cell carcinoma. Loricrin gene mutation that is occasionally observed in loricrin keratoderma such as Vohwinkel's syndrome was not detected in the present case.

Adenocarcinoma↗